##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1630600.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1630421
Sequences flagged as poor quality	0
Sequence length	43
%GC	44
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.07043272872467	33.0	31.0	34.0	30.0	34.0
2	32.23617703648321	33.0	31.0	34.0	30.0	34.0
3	32.33336359136689	34.0	31.0	34.0	30.0	34.0
4	35.905757470003145	37.0	35.0	37.0	35.0	37.0
5	35.881041154401224	37.0	35.0	37.0	35.0	37.0
6	35.97160119993548	37.0	35.0	37.0	35.0	37.0
7	35.91851613785642	37.0	35.0	37.0	35.0	37.0
8	35.934615660617716	37.0	35.0	37.0	35.0	37.0
9	37.62056732586246	39.0	37.0	39.0	35.0	39.0
10	37.55547800230738	39.0	37.0	39.0	35.0	39.0
11	37.65868324806906	39.0	37.0	39.0	35.0	39.0
12	37.61702100255088	39.0	37.0	39.0	35.0	39.0
13	37.65779881392597	39.0	37.0	39.0	35.0	39.0
14	38.93757501896749	40.0	38.0	41.0	36.0	41.0
15	38.935211212318784	40.0	38.0	41.0	36.0	41.0
16	38.88537132433893	40.0	38.0	41.0	35.0	41.0
17	38.90233504107221	40.0	38.0	41.0	36.0	41.0
18	38.91977286847998	40.0	38.0	41.0	36.0	41.0
19	38.99991842597709	40.0	39.0	41.0	35.0	41.0
20	38.978611045858706	40.0	39.0	41.0	35.0	41.0
21	38.942005776422164	40.0	38.0	41.0	35.0	41.0
22	38.86097210475086	40.0	38.0	41.0	35.0	41.0
23	38.800736742227926	40.0	38.0	41.0	35.0	41.0
24	38.753181540227956	40.0	38.0	41.0	35.0	41.0
25	38.69959906061072	40.0	38.0	41.0	35.0	41.0
26	38.55450647409473	40.0	38.0	41.0	34.0	41.0
27	38.412551114098754	40.0	38.0	41.0	34.0	41.0
28	38.312833311150925	40.0	38.0	41.0	34.0	41.0
29	38.216924340400425	40.0	38.0	41.0	34.0	41.0
30	38.107044131546395	40.0	37.0	41.0	34.0	41.0
31	38.02476354266781	40.0	37.0	41.0	33.0	41.0
32	37.89160407035974	40.0	37.0	41.0	33.0	41.0
33	37.78286405781083	40.0	37.0	41.0	33.0	41.0
34	37.692941270996876	40.0	37.0	41.0	33.0	41.0
35	37.59838532501728	40.0	37.0	41.0	33.0	41.0
36	37.523081461781956	40.0	37.0	41.0	33.0	41.0
37	37.35113200823591	40.0	36.0	41.0	32.0	41.0
38	37.1650303817235	40.0	36.0	41.0	32.0	41.0
39	37.04178675323735	40.0	36.0	41.0	31.0	41.0
40	36.81905225705508	40.0	35.0	41.0	31.0	41.0
41	36.702723407021864	40.0	35.0	41.0	30.0	41.0
42	36.50442125070764	39.0	35.0	41.0	30.0	41.0
43	35.764056645492175	38.0	35.0	40.0	28.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	1.0
8	1.0
9	7.0
10	7.0
11	9.0
12	6.0
13	8.0
14	2.0
15	4.0
16	10.0
17	16.0
18	36.0
19	77.0
20	189.0
21	384.0
22	684.0
23	1311.0
24	2125.0
25	3621.0
26	5550.0
27	8398.0
28	12275.0
29	17204.0
30	23294.0
31	30271.0
32	38733.0
33	50476.0
34	66731.0
35	88395.0
36	124370.0
37	201480.0
38	356093.0
39	598653.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	43.93417405688469	20.84504554345166	12.505359045301798	22.715421354361848
2	18.382982064141714	22.325583392264942	35.11976354573451	24.171670997858836
3	20.885709887200914	25.42570293194212	29.942573114551397	23.746014066305573
4	13.534786414061154	16.29585242093913	36.251127776200136	33.91823338879958
5	12.778724022813739	39.41190649531624	34.41190956200883	13.397459919861191
6	31.799762147322685	36.200895351568704	16.732794781225216	15.266547719883391
7	26.67875352439646	32.80766133409714	22.557732021361353	17.955853120145044
8	27.540555476162293	32.549875154944644	20.900859348597695	19.00871002029537
9	26.685868251206283	13.679656972033605	22.37906651104224	37.25540826571787
10	17.57926327003884	27.832136607661457	33.26380118999939	21.324798932300308
11	32.41696469807491	23.6093009106237	22.234073285366172	21.739661105935216
12	20.618784964128896	28.488592823571334	29.48146521665263	21.411156995647136
13	28.497363564379995	21.432746511483845	27.49565909663823	22.574230827497928
14	21.764746651325027	23.31686110519921	27.749887912385816	27.16850433108994
15	25.18153286789118	28.108322942356605	25.58093891087026	21.129205278881958
16	23.11133136778783	27.769024074150174	27.17549639019615	21.944148167865844
17	21.18287239921468	27.8518861079439	28.3271621256105	22.63807936723092
18	20.92404354458143	25.929315189144397	30.398038298083748	22.748602968190422
19	21.312286826531306	27.049884661691674	30.56265835633864	21.07517015543838
20	22.566441428318207	25.813026206114863	30.66257120093522	20.957961164631712
21	22.897582894234066	26.72223922532892	29.594748840943534	20.78542903949348
22	22.933953868356703	26.44991692329772	29.81242268101306	20.803706527332512
23	22.2002169991677	26.36883357120646	30.00041093680712	21.430538492818727
24	21.27597718626048	27.387282180492033	29.514340161222165	21.822400472025326
25	21.66624448532005	27.055036705243612	29.93331170292826	21.345407106508073
26	21.200720550091052	27.870470265041973	29.442518220754028	21.48629096411295
27	21.580377092787693	27.370476705096415	29.63222382439873	21.416922377717164
28	21.07142879047804	27.303561472772987	30.185761836973395	21.43924789977558
29	20.585235347189467	27.792576273244762	30.73059044259121	20.89159793697456
30	20.50887470168748	28.412722848883813	30.36596069358773	20.712441755840977
31	21.192133810837817	28.594393717941564	29.243673873189806	20.969798598030813
32	20.490658547700257	27.827965905738456	30.00292562473128	21.678449921830005
33	20.067025633256684	27.82348853455641	30.324192340505917	21.78529349168098
34	21.09467432031359	27.56257432896166	30.03665924322614	21.306092107498614
35	20.497527939102845	27.80478170975472	30.25022371522447	21.447466635917962
36	20.492437229402714	28.491230179199118	29.22165502039044	21.794677571007735
37	21.229670128144818	26.824237420887	30.250898387594372	21.695194063373815
38	20.455636918317417	26.881584572328247	30.355227269521183	22.30755123983315
39	20.48894119984961	27.674263273105538	30.519724660072463	21.317070866972397
40	19.940064560012413	27.001430918762697	30.901282552175175	22.15722196904971
41	19.75146296570027	27.20076593714139	31.012726160911814	22.03504493624653
42	19.942640581788385	27.336007080379854	30.874479658934717	21.846872678897043
43	19.370027741301175	26.56375255225491	30.744206557692767	23.32201314875115
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	480.0
1	468.0
2	456.0
3	1200.5
4	1945.0
5	1945.0
6	2930.5
7	3916.0
8	3905.5
9	3895.0
10	5798.0
11	7701.0
12	7701.0
13	13250.0
14	18799.0
15	24493.5
16	30188.0
17	29195.0
18	28202.0
19	28202.0
20	33087.5
21	37973.0
22	34529.5
23	31086.0
24	34234.0
25	37382.0
26	37382.0
27	39334.5
28	41287.0
29	45617.0
30	49947.0
31	56084.0
32	62221.0
33	62221.0
34	69515.5
35	76810.0
36	82083.0
37	87356.0
38	92687.0
39	98018.0
40	98018.0
41	101039.5
42	104061.0
43	105687.0
44	107313.0
45	105198.5
46	103084.0
47	103084.0
48	104117.0
49	105150.0
50	102667.0
51	100184.0
52	97554.0
53	94924.0
54	94924.0
55	89882.0
56	84840.0
57	79436.0
58	74032.0
59	68648.5
60	63265.0
61	63265.0
62	59175.0
63	55085.0
64	48140.5
65	41196.0
66	36227.5
67	31259.0
68	31259.0
69	25984.5
70	20710.0
71	16820.5
72	12931.0
73	9556.5
74	6182.0
75	6182.0
76	4584.5
77	2987.0
78	2519.0
79	2051.0
80	1630.5
81	1210.0
82	1210.0
83	1015.5
84	821.0
85	752.0
86	683.0
87	574.0
88	465.0
89	465.0
90	367.5
91	270.0
92	157.5
93	45.0
94	26.0
95	7.0
96	7.0
97	5.5
98	4.0
99	2.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	1630421.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	54.851539469252316
#Duplication Level	Percentage of deduplicated	Percentage of total
1	87.18231887673073	47.82084404887938
2	8.308051902353322	9.1141887366906
3	1.6949130957281904	2.7890577770186242
4	0.638950203992857	1.4018960933280404
5	0.34621035184272625	0.9495085389382518
6	0.23455956800140249	0.7719572041271822
7	0.16822987382986218	0.6459367295000217
8	0.13759773205291728	0.6037957944464158
9	0.12103867219477729	0.5975241754677949
>10	0.9129266208703943	10.282661960049925
>50	0.1297953535352911	4.995167867187327
>100	0.1124192212639431	11.908830400998712
>500	0.010301246030382743	3.845460281726797
>1k	0.0022394013109527703	1.7509459396241818
>5k	1.1197006554763852E-4	0.4342817615168048
>10k+	3.359101966429156E-4	2.0879426904999714
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	12850	0.7881399957434306	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	11022	0.6760217146368943	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	10124	0.6209439157125675	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	7071	0.43369166614021776	No Hit
GTGTAGCGCGCGTGCAGCCCCGGACATCTAAGGGCATCACAGA	2120	0.13002776583471387	No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	2074	0.12720640865150779	No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1926	0.11812899858380135	No Hit
AACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1817	0.11144360873663918	No Hit
GGGTAGGCACACGCTGAGCCAGTCAGTGTAGCGCGCGTGCAGC	1770	0.1085609177016243	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	1.226677036176546E-4	0.0
2	0.0	0.0	0.0	0.001656013998838337	0.0
3	0.0	0.0	0.0	0.0024533540723530914	0.0
4	0.0	0.0	0.0	0.004048034219382601	0.0
5	0.0	0.0	0.0	0.004232035774809083	0.0
6	0.0	0.0	0.0	0.004416037330235565	0.0
7	0.0	0.0	0.0	0.005029375848323838	0.0
8	0.0	0.0	0.0	0.005336045107367974	0.0
9	0.0	0.0	0.0	0.00950674703036823	0.0
10	0.0	0.0	0.0	0.012818775028044904	0.0
11	0.0	0.0	0.0	0.014290787471456758	0.0
12	0.0	0.0	0.0	0.01656013998838337	0.0
13	0.0	0.0	0.0	0.01833882169083936	0.0
14	0.0	0.0	0.0	0.02085350961500128	0.0
15	0.0	0.0	0.0	0.023858868353633815	0.0
16	0.0	0.0	0.0	0.030176255089943028	0.0
17	0.0	0.0	0.0	0.03802698812147292	0.0
18	0.0	0.0	0.0	0.04232035774809083	0.0
19	0.0	0.0	0.0	0.04753373515184115	0.0
20	0.0	0.0	0.0	0.05299244796282678	0.0
21	0.0	0.0	0.0	0.06372587202937155	0.0
22	0.0	0.0	0.0	0.08145135520212264	0.0
23	0.0	0.0	0.0	0.10567822666660942	0.0
24	0.0	0.0	0.0	0.14444122100978826	0.0
25	0.0	0.0	0.0	0.15566531589080365	0.0
26	0.0	0.0	0.0	0.17559881772867253	0.0
27	0.0	0.0	0.0	0.19994835689677698	0.0
28	0.0	0.0	0.0	0.24012202983155884	0.0
29	0.0	0.0	0.0	0.3002292046042096	0.0
30	0.0	0.0	0.0	0.3887952866161562	0.0
31	0.0	0.0	0.0	0.69037383596016	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
ATCCGTA	55	5.144363E-4	20.181818	12
TCCGTAT	55	5.144363E-4	20.181818	13
CCGTATT	60	9.239079E-4	18.5	14
CCGCTTA	95	3.608262E-6	17.526314	25
CGTAAGA	65	0.0015803176	17.076923	2
AAGTACG	250	0.0	17.02	35
AATGCGT	180	1.0913936E-11	16.444445	35
ACGGACC	195	1.8189894E-12	16.128206	8
ATTCCGT	150	4.6748028E-9	16.033333	29
GGTATCA	6365	0.0	15.927729	1
CATCCGT	70	0.0025931173	15.857143	11
TCTATAC	175	1.3278623E-10	15.857142	3
GCCGTCT	155	7.214112E-9	15.516129	36
GACGGAC	205	5.456968E-12	15.341464	7
CGCAAGA	220	1.8189894E-12	15.136364	2
CTAATAC	480	0.0	15.031249	3
AAATGCG	185	3.0559022E-10	15.0	34
GCGAACT	325	0.0	14.8	28
CGTTTAG	225	1.8189894E-12	14.8	26
TCCGAAT	175	2.239176E-9	14.799999	12
>>END_MODULE