FastQCFastQC Report
Fri 10 Feb 2017
ERR1630599.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1630599.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences378067
Sequences flagged as poor quality0
Sequence length43
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT101252.678096739466814No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT95252.5193947104613734No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT72211.9099789190804806No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT46351.2259731740670303No Hit
CTTATACACATCTCCGAGCCCACGAGACGGAGCTACATCTCGT12360.3269261797512081No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT11820.3126429971407184No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT11150.2949212705684442No Hit
AACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT9850.26053583095059873No Hit
TCTCCGAGCCCACGAGACGGAGCTACATCTCGTATGCCGTCTT9000.23805304350816128TruSeq Adapter, Index 11 (95% over 23bp)
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT7750.20499012079869444No Hit
GTGTAGCGCGCGTGCAGCCCCGGACATCTAAGGGCATCACAGA7180.1899134280431775No Hit
GAACAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAA6920.18303634011960843No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTG6690.17695276234106652No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTT6340.1676951439824158No Hit
GGGTAGGCACACGCTGAGCCAGTCAGTGTAGCGCGCGTGCAGC5850.15473447828030482No Hit
ATACACATCTCCGAGCCCACGAGACGGAGCTACATCTCGTATG5730.151560437700196No Hit
CTCTATGGGCAGTCGGTGATTCCTCGCCTTATTGATATGCCCA5650.14944441064679012No Hit
GTACATGGGAATGGTATCAACGCAAAAAAAAAAAAAAAAAAAA5620.1486509005017629No Hit
CTTCTTAGAGGGACAAGTGGCGTTCAGCCACCCGAGATTGAGC5550.14679937683003277No Hit
ACACATCTCCGAGCCCACGAGACGGAGCTACATCTCGTATGCC5380.14230281934154526No Hit
TGCCTACCCTACGCCGGCAGGCGCGGGTAACCCGTTGAACCCC5350.14150930919651808No Hit
TCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTT4850.12828414011273134No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT4770.12616811305932546No Hit
GGGTTCAACGGGTTACCCGCGCCTGCCGGCGTAGGGTAGGCAC4670.12352307924256813No Hit
TCTTAGAGGGACAAGTGGCGTTCAGCCACCCGAGATTGAGCAA4640.1227295690975409No Hit
GGATTACTCCGGTCTGAACTCAGATCACGTAGGACTTTAATCG4620.12220056233418944No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGG4490.1187620183724049No Hit
CATCTCCGAGCCCACGAGACGGAGCTACATCTCGTATGCCGTC4170.11029791015878138TruSeq Adapter, Index 11 (95% over 21bp)
GGTCTGTGATGCCCTTAGATGTCCGGGGCTGCACGCGCGCTAC4140.10950440001375418No Hit
GGTCGGGAGTGGGTAATTTGCGCGCCTGCTGCCTTCCTTGGAT4050.10712386957867258No Hit
CAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT3920.10368532561688801No Hit
GTACATGGGGAATAATTGCAATCCCCGATCCCCATCACGAATG3850.10183380194515787No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGACCGT251.2320589E-437.06
GTCTGGG250.00549173629.69
TTGTCTG250.00549173629.67
GCTCTCG250.00549173629.614
GCAGCGT250.00549173629.61
CCACACT250.00549173629.632
CAGTATT405.9297512E-527.7514
CCGTCTT1900.027.26315537
GAACTAA358.8583416E-426.4285721
TCTAAGA358.8583416E-426.42857230
GCCGTCT2050.025.26829336
TAATTGT451.3209188E-424.6666664
ATGCAGG751.3675162E-824.66666636
AGGCACC551.8982835E-523.54545423
TAATCAT400.00192880423.1255
AATGCAG802.7135684E-823.12535
GAGAATA400.00192880423.12534
ATTAGAG502.6974562E-422.218
CCAGTAT502.6974562E-422.213
TTTAATG855.1575626E-821.76470632
Produced by FastQC (version 0.11.5)