##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1630599.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	378067
Sequences flagged as poor quality	0
Sequence length	43
%GC	42
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.9572403833183	33.0	31.0	34.0	30.0	34.0
2	32.05196697939783	33.0	31.0	34.0	30.0	34.0
3	32.06723411458815	33.0	31.0	34.0	30.0	34.0
4	35.73114024762807	37.0	35.0	37.0	33.0	37.0
5	35.742685820238215	37.0	35.0	37.0	35.0	37.0
6	35.886652365850495	37.0	35.0	37.0	35.0	37.0
7	35.80437858898026	37.0	35.0	37.0	35.0	37.0
8	35.8648705123695	37.0	35.0	37.0	35.0	37.0
9	37.47231046348928	39.0	37.0	39.0	35.0	39.0
10	37.40867359489191	39.0	37.0	39.0	34.0	39.0
11	37.54605929636811	39.0	37.0	39.0	35.0	39.0
12	37.51764105304086	39.0	37.0	39.0	35.0	39.0
13	37.56703970460262	39.0	37.0	39.0	35.0	39.0
14	38.671119140258206	40.0	38.0	41.0	34.0	41.0
15	38.630041765083966	40.0	38.0	41.0	34.0	41.0
16	38.52253172056805	40.0	38.0	41.0	34.0	41.0
17	38.64781903736639	40.0	38.0	41.0	35.0	41.0
18	38.72656698415889	40.0	38.0	41.0	35.0	41.0
19	38.849889040831386	40.0	38.0	41.0	35.0	41.0
20	38.84142493261776	40.0	38.0	41.0	35.0	41.0
21	38.79933715452552	40.0	38.0	41.0	35.0	41.0
22	38.66593222894355	40.0	38.0	41.0	35.0	41.0
23	38.564429056225485	40.0	38.0	41.0	34.0	41.0
24	38.46761288343072	40.0	38.0	41.0	34.0	41.0
25	38.36510988792992	40.0	38.0	41.0	34.0	41.0
26	38.11459873514483	40.0	37.0	41.0	34.0	41.0
27	37.883227047057794	40.0	36.0	41.0	33.0	41.0
28	37.70513956520934	40.0	36.0	41.0	33.0	41.0
29	37.52036014780449	39.0	36.0	41.0	33.0	41.0
30	37.25952013796496	39.0	35.0	41.0	33.0	41.0
31	37.00839269230057	39.0	35.0	41.0	32.0	41.0
32	36.71595775351988	39.0	35.0	41.0	31.0	41.0
33	36.44982503101302	38.0	35.0	41.0	31.0	41.0
34	36.238526504561364	38.0	35.0	41.0	31.0	41.0
35	35.977662689417485	38.0	35.0	41.0	30.0	41.0
36	35.749957018200476	38.0	35.0	41.0	29.0	41.0
37	35.39002875151759	38.0	35.0	40.0	27.0	41.0
38	35.02289805775167	38.0	35.0	40.0	25.0	41.0
39	34.6917160185893	38.0	35.0	40.0	23.0	41.0
40	34.242131685653604	37.0	34.0	40.0	20.0	41.0
41	33.904104298973465	37.0	33.0	40.0	18.0	41.0
42	33.43655754138817	37.0	33.0	40.0	14.0	41.0
43	32.56246115106582	36.0	32.0	40.0	10.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
10	4.0
11	6.0
12	2.0
13	5.0
14	6.0
15	2.0
16	6.0
17	12.0
18	23.0
19	42.0
20	89.0
21	210.0
22	355.0
23	579.0
24	964.0
25	1552.0
26	2458.0
27	3575.0
28	5132.0
29	6764.0
30	8606.0
31	10415.0
32	12614.0
33	15599.0
34	19878.0
35	25080.0
36	34583.0
37	60687.0
38	76964.0
39	91855.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	48.5628208756649	18.92283642846374	13.729577032642363	18.784765663229006
2	18.968595513493643	22.958364522690424	33.74587044095359	24.32716952286235
3	23.064430378742394	27.333779462370426	29.998122025990103	19.603668132897077
4	12.799583142670478	16.50977207743601	31.56213052183872	39.12851425805479
5	11.524147836230087	42.98127051554354	31.044497403899307	14.450084244327064
6	31.115384310188404	31.61423768802884	18.30892407959436	18.961453922188394
7	25.74094009791915	34.62269915120865	21.02748983645756	18.60887091441464
8	33.35599245636355	28.41374676975245	19.411903181182172	18.818357592701823
9	28.859170464494387	10.672975953997572	22.324614420195363	38.14323916131268
10	20.748703272171333	29.513551830760154	27.809621046005073	21.928123851063436
11	30.771265410628274	24.309976803053427	19.1783466951625	25.740411091155803
12	22.14342960374748	31.609476627158678	26.781496401431493	19.465597367662347
13	31.341799204902838	19.285206061359496	29.257777060679718	20.11521767305795
14	23.401936693760632	22.43808637093425	30.162114122629056	23.99786281267606
15	28.23652950402971	24.63663847942296	28.189183398709755	18.93764861783758
16	22.27330076415027	24.455189159593406	30.61467940867624	22.656830667580085
17	20.121301250836492	28.44363565188181	29.5294220336607	21.905641063620997
18	19.268542348313925	22.0058878452761	34.57429503236199	24.151274774047987
19	20.24852737742252	25.62746814718026	35.4974118344103	18.626592640986914
20	21.633467083876667	21.636641124456776	37.396546114842074	19.33334567682448
21	23.429709548836584	23.298515871525417	34.645181938651085	18.626592640986914
22	23.692361406840586	23.748171620374166	33.22559228919742	19.33387468358783
23	21.051824147571725	24.08223939143062	34.21271890961126	20.653217551386394
24	19.415870731907308	26.319144490262307	34.52192336279019	19.74306141504019
25	19.273303409184088	25.68327836071384	34.61635107004843	20.427067160053642
26	20.60058137843292	25.970793536595366	33.988420041950235	19.440205043021475
27	20.28264831365869	26.454570221680285	33.89425683807368	19.36852462658735
28	18.64352085741416	26.29295865547641	35.33844530202319	19.72507518508624
29	19.01250307485181	26.808475746362415	35.69076380641526	18.488257372370505
30	17.299050168356402	27.93631816582775	36.18009506251537	18.58453660330047
31	18.467097101836448	28.796747666418916	33.656732801328864	19.079422430415775
32	17.482351011857684	28.33386674848638	34.72188791933705	19.461894320318883
33	17.15806986592324	27.99318639288803	35.017867203432196	19.830876537756538
34	18.575543488323497	28.181512800641155	34.421412077753416	18.821531633281932
35	17.22181518090709	29.3768035824338	34.12014272602475	19.281238510634356
36	17.488434589636228	29.419388626883595	32.84444291620268	20.247733867277493
37	17.715642994495685	28.593080062528596	33.83342106028825	19.85785588268746
38	17.59264892201647	27.42371061214018	33.723123150129474	21.26051731571388
39	18.204180740450767	28.302919852830318	33.803267674777224	19.68963173194169
40	16.878489791491987	28.261921828670577	33.88473471633335	20.974853663504085
41	16.86447111226317	28.556049589093995	33.418150751057354	21.16132854758548
42	16.81474447650813	28.92979286740181	33.90801101392081	20.347451642169247
43	16.230985513149783	28.209814662480458	33.052607077581484	22.506592746788268
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	40.0
1	84.0
2	128.0
3	549.0
4	970.0
5	970.0
6	1576.0
7	2182.0
8	2303.5
9	2425.0
10	3395.0
11	4365.0
12	4365.0
13	7712.0
14	11059.0
15	16185.0
16	21311.0
17	18842.5
18	16374.0
19	16374.0
20	17077.0
21	17780.0
22	12425.0
23	7070.0
24	6438.0
25	5806.0
26	5806.0
27	6131.5
28	6457.0
29	6864.0
30	7271.0
31	7695.5
32	8120.0
33	8120.0
34	9027.5
35	9935.0
36	10517.0
37	11099.0
38	13093.5
39	15088.0
40	15088.0
41	16640.0
42	18192.0
43	19575.5
44	20959.0
45	22132.0
46	23305.0
47	23305.0
48	25440.5
49	27576.0
50	25867.0
51	24158.0
52	23182.0
53	22206.0
54	22206.0
55	19505.0
56	16804.0
57	16345.0
58	15886.0
59	14775.0
60	13664.0
61	13664.0
62	12837.0
63	12010.0
64	11201.5
65	10393.0
66	9879.5
67	9366.0
68	9366.0
69	7666.0
70	5966.0
71	5119.0
72	4272.0
73	3271.5
74	2271.0
75	2271.0
76	1857.5
77	1444.0
78	1199.5
79	955.0
80	738.5
81	522.0
82	522.0
83	422.0
84	322.0
85	241.0
86	160.0
87	130.5
88	101.0
89	101.0
90	71.5
91	42.0
92	25.5
93	9.0
94	6.0
95	3.0
96	3.0
97	2.0
98	1.0
99	0.5
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	378067.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	39.00234127514075
#Duplication Level	Percentage of deduplicated	Percentage of total
1	79.45153721306293	30.98795969218426
2	9.785038826658646	7.632788474156866
3	3.2952346935784056	3.855656043018865
4	1.8026757573683554	2.8123430038921367
5	1.099687493424768	2.1445193457278457
6	0.7437289962124278	1.7404303275896986
7	0.5286535371075854	1.4433107969406234
8	0.4194832392050903	1.3088662763742747
9	0.3140139060569948	1.102254977625741
>10	2.13453858301512	16.159443484187097
>50	0.2378477859559228	6.465804502014503
>100	0.17328910119645805	12.598445804232647
>500	0.009513911438236912	2.4612199469443197
>1k	0.002718260410924832	2.1648981829249623
>5k	0.001359130205462416	4.4384653490770605
>10k+	6.79565102731208E-4	2.6835937931091145
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	10125	2.678096739466814	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	9525	2.5193947104613734	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	7221	1.9099789190804806	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	4635	1.2259731740670303	No Hit
CTTATACACATCTCCGAGCCCACGAGACGGAGCTACATCTCGT	1236	0.3269261797512081	No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1182	0.3126429971407184	No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1115	0.2949212705684442	No Hit
AACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	985	0.26053583095059873	No Hit
TCTCCGAGCCCACGAGACGGAGCTACATCTCGTATGCCGTCTT	900	0.23805304350816128	TruSeq Adapter, Index 11 (95% over 23bp)
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	775	0.20499012079869444	No Hit
GTGTAGCGCGCGTGCAGCCCCGGACATCTAAGGGCATCACAGA	718	0.1899134280431775	No Hit
GAACAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAA	692	0.18303634011960843	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTG	669	0.17695276234106652	No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTT	634	0.1676951439824158	No Hit
GGGTAGGCACACGCTGAGCCAGTCAGTGTAGCGCGCGTGCAGC	585	0.15473447828030482	No Hit
ATACACATCTCCGAGCCCACGAGACGGAGCTACATCTCGTATG	573	0.151560437700196	No Hit
CTCTATGGGCAGTCGGTGATTCCTCGCCTTATTGATATGCCCA	565	0.14944441064679012	No Hit
GTACATGGGAATGGTATCAACGCAAAAAAAAAAAAAAAAAAAA	562	0.1486509005017629	No Hit
CTTCTTAGAGGGACAAGTGGCGTTCAGCCACCCGAGATTGAGC	555	0.14679937683003277	No Hit
ACACATCTCCGAGCCCACGAGACGGAGCTACATCTCGTATGCC	538	0.14230281934154526	No Hit
TGCCTACCCTACGCCGGCAGGCGCGGGTAACCCGTTGAACCCC	535	0.14150930919651808	No Hit
TCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	485	0.12828414011273134	No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	477	0.12616811305932546	No Hit
GGGTTCAACGGGTTACCCGCGCCTGCCGGCGTAGGGTAGGCAC	467	0.12352307924256813	No Hit
TCTTAGAGGGACAAGTGGCGTTCAGCCACCCGAGATTGAGCAA	464	0.1227295690975409	No Hit
GGATTACTCCGGTCTGAACTCAGATCACGTAGGACTTTAATCG	462	0.12220056233418944	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGG	449	0.1187620183724049	No Hit
CATCTCCGAGCCCACGAGACGGAGCTACATCTCGTATGCCGTC	417	0.11029791015878138	TruSeq Adapter, Index 11 (95% over 21bp)
GGTCTGTGATGCCCTTAGATGTCCGGGGCTGCACGCGCGCTAC	414	0.10950440001375418	No Hit
GGTCGGGAGTGGGTAATTTGCGCGCCTGCTGCCTTCCTTGGAT	405	0.10712386957867258	No Hit
CAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	392	0.10368532561688801	No Hit
GTACATGGGGAATAATTGCAATCCCCGATCCCCATCACGAATG	385	0.10183380194515787	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0015870202900544084	0.0
3	0.0	0.0	0.0	0.0021160270534058777	0.0
4	0.0	0.0	0.0	0.002909537198433082	0.0
5	0.0	0.0	0.0	0.003174040580108817	0.0
6	0.0	0.0	0.0	0.003174040580108817	0.0
7	0.0	0.0	0.0	0.003967550725136021	0.0
8	0.0	0.0	0.0	0.0042320541068117555	0.0
9	0.0	0.0	0.0	0.005554571015190429	0.0
10	0.0	0.0	0.0	0.008199604831947777	0.0
11	0.0	0.0	0.0	0.00899311497697498	0.0
12	0.0	0.0	0.0	0.011638148793732327	0.0
13	0.0	0.0	0.0	0.012960665702111001	0.0
14	0.0	0.0	0.0	0.015605699518868348	0.0
15	0.0	0.0	0.0	0.023011794205788923	0.0
16	0.0	0.0	0.0	0.04681709855660505	0.0
17	0.0	0.0	0.0	0.07247392657915132	0.0
18	0.0	0.0	0.0	0.08596359904461379	0.0
19	0.0	0.0	0.0	0.10104029180013066	0.0
20	0.0	0.0	0.0	0.11400095750224166	0.0
21	0.0	0.0	0.0	0.151560437700196	0.0
22	0.0	2.645033816757347E-4	0.0	0.21451224253902085	0.0
23	0.0	2.645033816757347E-4	0.0	0.3139655140490971	0.0
24	0.0	2.645033816757347E-4	0.0	0.454681313100588	0.0
25	0.0	2.645033816757347E-4	0.0	0.48853774595508204	0.0
26	0.0	2.645033816757347E-4	0.0	0.540380408763526	0.0
27	0.0	2.645033816757347E-4	0.0	0.5864039971751038	0.0
28	0.0	2.645033816757347E-4	0.0	0.6419497073270082	0.0
29	0.0	2.645033816757347E-4	0.0	0.7411384754554087	0.0
30	0.0	2.645033816757347E-4	0.0	0.9204717682315569	0.0
31	0.0	2.645033816757347E-4	0.0	1.4481560146746477	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGACCGT	25	1.2320589E-4	37.0	6
GTCTGGG	25	0.005491736	29.6	9
TTGTCTG	25	0.005491736	29.6	7
GCTCTCG	25	0.005491736	29.6	14
GCAGCGT	25	0.005491736	29.6	1
CCACACT	25	0.005491736	29.6	32
CAGTATT	40	5.9297512E-5	27.75	14
CCGTCTT	190	0.0	27.263155	37
GAACTAA	35	8.8583416E-4	26.428572	1
TCTAAGA	35	8.8583416E-4	26.428572	30
GCCGTCT	205	0.0	25.268293	36
TAATTGT	45	1.3209188E-4	24.666666	4
ATGCAGG	75	1.3675162E-8	24.666666	36
AGGCACC	55	1.8982835E-5	23.545454	23
TAATCAT	40	0.001928804	23.125	5
AATGCAG	80	2.7135684E-8	23.125	35
GAGAATA	40	0.001928804	23.125	34
ATTAGAG	50	2.6974562E-4	22.2	18
CCAGTAT	50	2.6974562E-4	22.2	13
TTTAATG	85	5.1575626E-8	21.764706	32
>>END_MODULE