FastQCFastQC Report
Fri 10 Feb 2017
ERR1630598.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1630598.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1079085
Sequences flagged as poor quality0
Sequence length43
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT44170.4093282734909669No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT41990.38912597246741454No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT34150.3164718256671161No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT20810.1928485707798737No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AACTCCG450.003825659220.5555557
CTATCGC555.142814E-420.18181832
AAGACGG2250.018.9111125
CGGATTC500.00703460918.516
CGAATTA1103.8509825E-818.515
TATCGCC609.236315E-418.533
TCTATCG609.236315E-418.531
GGTATCA22000.017.4909081
TTATACC752.0671188E-417.2666664
GACGGAC2500.017.027
TCTAATA1757.2759576E-1216.9142862
TACCGTC1107.806502E-716.8181827
CGCAAGA2650.016.7547152
TAATACT1708.54925E-1116.323534
ATACCGT1251.6573176E-716.286
AATACTG1951.8189894E-1216.1282065
CACACTA1151.2419459E-616.08695610
GCGCAAG2650.016.0566021
CGCCTAT700.002592356415.85714236
GTAGCAC700.002592356415.8571423
Produced by FastQC (version 0.11.5)