##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1630598.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1079085
Sequences flagged as poor quality	0
Sequence length	43
%GC	48
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.679661009095668	31.0	31.0	34.0	30.0	34.0
2	31.864108017440703	33.0	31.0	34.0	30.0	34.0
3	31.956212902598036	33.0	31.0	34.0	30.0	34.0
4	35.62999485675364	37.0	35.0	37.0	33.0	37.0
5	35.53491986266142	37.0	35.0	37.0	33.0	37.0
6	35.606546286900475	37.0	35.0	37.0	33.0	37.0
7	35.55757238771737	37.0	35.0	37.0	33.0	37.0
8	35.55264784516512	37.0	35.0	37.0	33.0	37.0
9	37.20109259233517	39.0	37.0	39.0	34.0	39.0
10	37.04386772126385	39.0	37.0	39.0	33.0	39.0
11	37.1662890319113	39.0	37.0	39.0	33.0	39.0
12	37.09950837978472	39.0	37.0	39.0	33.0	39.0
13	37.1551870334589	39.0	37.0	39.0	33.0	39.0
14	38.33826714299615	40.0	38.0	41.0	34.0	41.0
15	38.33006760357154	40.0	38.0	41.0	34.0	41.0
16	38.27345482515279	40.0	38.0	41.0	33.0	41.0
17	38.248302033667414	40.0	38.0	41.0	33.0	41.0
18	38.230549956676256	40.0	38.0	41.0	33.0	41.0
19	38.286717913788074	40.0	38.0	41.0	34.0	41.0
20	38.24256291209682	40.0	38.0	41.0	34.0	41.0
21	38.1994254391452	40.0	38.0	41.0	34.0	41.0
22	38.12222206777038	40.0	38.0	41.0	33.0	41.0
23	38.05539137324678	40.0	37.0	41.0	33.0	41.0
24	37.98083005509297	40.0	37.0	41.0	33.0	41.0
25	37.917753467057736	40.0	37.0	41.0	33.0	41.0
26	37.75497945018233	40.0	37.0	41.0	33.0	41.0
27	37.60647771028232	40.0	37.0	41.0	32.0	41.0
28	37.48640097860687	39.0	37.0	41.0	32.0	41.0
29	37.38686572420152	39.0	36.0	41.0	32.0	41.0
30	37.271876636224206	39.0	36.0	41.0	31.0	41.0
31	37.227807818661184	39.0	36.0	40.0	31.0	41.0
32	37.10362946385132	39.0	36.0	40.0	31.0	41.0
33	37.01562805525052	39.0	36.0	40.0	31.0	41.0
34	36.946928184526705	39.0	36.0	40.0	31.0	41.0
35	36.87074234189151	39.0	35.0	40.0	31.0	41.0
36	36.83506211280854	39.0	35.0	40.0	31.0	41.0
37	36.68371536996622	39.0	35.0	40.0	30.0	41.0
38	36.510061765291894	39.0	35.0	40.0	30.0	41.0
39	36.44370369340692	39.0	35.0	40.0	30.0	41.0
40	36.23992363900898	39.0	35.0	40.0	30.0	41.0
41	36.15063595546226	38.0	35.0	40.0	29.0	41.0
42	35.99260762590528	38.0	35.0	40.0	29.0	41.0
43	35.11004323107077	38.0	34.0	40.0	26.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	1.0
9	0.0
10	8.0
11	7.0
12	2.0
13	0.0
14	3.0
15	6.0
16	15.0
17	33.0
18	63.0
19	130.0
20	241.0
21	470.0
22	859.0
23	1461.0
24	2310.0
25	3673.0
26	5474.0
27	7876.0
28	10896.0
29	14973.0
30	19843.0
31	25506.0
32	32656.0
33	41852.0
34	55169.0
35	73603.0
36	106575.0
37	166397.0
38	289831.0
39	219152.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	43.25859408665675	19.523392503834266	12.4031007751938	24.814912634315185
2	19.574454283026824	21.47995755663363	33.45000625529963	25.495581905039916
3	20.9776801642132	23.54791327837937	29.365434604317546	26.10897195308989
4	15.029770592678057	15.728788742314089	34.14179605869788	35.09964460630998
5	14.613677328477367	37.044255086485315	33.75248474401924	14.589582841018084
6	34.65862281469949	34.60774637771816	15.511474999652483	15.222155807929866
7	28.998364354985938	30.52567684658762	21.428988448546686	19.04697034987976
8	27.938762933411176	32.103124406325726	20.183488789113	19.774623871150094
9	26.978134252630703	13.49986331011922	20.0022241065347	39.519778330715376
10	17.664966151878676	26.34519060129647	32.31626794923477	23.673575297590087
11	35.707845072445636	21.68930158421255	21.094631099496333	21.50822224384548
12	22.053777042587004	25.872382620460854	28.18295129670044	23.890889040251693
13	30.16500090354328	19.502819518388264	25.47834507939597	24.853834498672487
14	23.411594082023196	21.12308112891941	25.017769684501225	30.447555104556173
15	26.254650931112934	27.12242316406956	22.564487505618185	24.05843839919932
16	25.567123998572868	25.933267536848348	24.279551657191046	24.220056807387742
17	23.758554701436864	26.236950749940924	25.566567971939193	24.437926576683022
18	23.639194317407807	24.358322096961775	27.328338360740812	24.674145224889603
19	24.266948386827732	24.968932011843368	27.171353507833025	23.59276609349588
20	24.911939281891602	23.94398958376773	27.02261638332476	24.121454751015907
21	25.33581691896375	24.7445752651552	26.216192422283697	23.703415393597353
22	25.104973194882703	24.720666119907143	26.189688486078484	23.98467219913167
23	24.228953233526553	24.682300282183515	26.73857944462206	24.350167039667866
24	24.159727917633923	25.39401437328848	26.375030697303735	24.071227011773864
25	24.54644444135541	24.7908181468559	26.489757526052166	24.172979885736527
26	24.24127849057303	25.606045862930166	26.254836273324162	23.89783937317264
27	24.636984111538943	25.008317231728732	25.91983022653452	24.434868430197806
28	23.810079836157485	24.992841157091426	27.132246301264495	24.064832705486594
29	23.424197352386514	25.48325664799344	27.109912564811857	23.982633434808193
30	23.38527548802921	26.093032522924513	27.059499483358586	23.46219250568769
31	24.03165644967727	25.894716356913495	26.10943530861795	23.96419188479128
32	23.490271850688316	25.437940477348864	26.409133664169183	24.66265400779364
33	23.240801234379127	25.26168003447365	27.115936186676677	24.381582544470547
34	24.292154927554364	24.971341460589297	26.724586107674558	24.011917504181785
35	24.06724215423252	25.244906564357766	27.224454051349063	23.463397230060654
36	23.411130726495134	25.71002284342753	26.549808402489145	24.329038027588187
37	24.125717621874088	24.742721843042947	26.945699365666282	24.185861169416683
38	23.41205743755126	24.670345709559488	27.618028236885877	24.299568616003373
39	23.578587414337147	24.831593433325455	27.263375915706362	24.326443236631036
40	23.13793630714911	24.73892232771283	27.994643610095586	24.128497755042467
41	22.445590477117186	24.901838131379826	28.28377745960698	24.368793931896004
42	23.23162679492348	24.85772668510822	28.052285037786646	23.858361482181664
43	22.644833354184332	24.134428705801675	28.22122446331846	24.999513476695533
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	71.0
1	86.5
2	102.0
3	309.5
4	517.0
5	517.0
6	793.5
7	1070.0
8	1122.5
9	1175.0
10	1667.5
11	2160.0
12	2160.0
13	3772.0
14	5384.0
15	7853.5
16	10323.0
17	9998.0
18	9673.0
19	9673.0
20	11272.5
21	12872.0
22	12239.5
23	11607.0
24	13149.5
25	14692.0
26	14692.0
27	16383.0
28	18074.0
29	21281.0
30	24488.0
31	27967.5
32	31447.0
33	31447.0
34	35141.0
35	38835.0
36	42799.0
37	46763.0
38	50938.5
39	55114.0
40	55114.0
41	58135.0
42	61156.0
43	61986.5
44	62817.0
45	64234.5
46	65652.0
47	65652.0
48	68039.5
49	70427.0
50	71536.0
51	72645.0
52	74952.5
53	77260.0
54	77260.0
55	74702.0
56	72144.0
57	69147.5
58	66151.0
59	62321.0
60	58491.0
61	58491.0
62	54964.0
63	51437.0
64	45923.0
65	40409.0
66	36035.0
67	31661.0
68	31661.0
69	27395.5
70	23130.0
71	20183.5
72	17237.0
73	13552.5
74	9868.0
75	9868.0
76	7625.0
77	5382.0
78	4485.5
79	3589.0
80	2877.0
81	2165.0
82	2165.0
83	1670.0
84	1175.0
85	1013.5
86	852.0
87	763.0
88	674.0
89	674.0
90	508.0
91	342.0
92	193.0
93	44.0
94	24.5
95	5.0
96	5.0
97	4.0
98	3.0
99	2.5
100	2.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	1079085.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	57.10526951132396
#Duplication Level	Percentage of deduplicated	Percentage of total
1	86.81795345782169	49.57762630630487
2	7.920351111433586	9.045875696854585
3	1.9056674427496134	3.264709587515166
4	0.8398706500428229	1.91844159301385
5	0.48585337220189734	1.387239388128747
6	0.3017961082455281	1.034048885929775
7	0.2126890388929241	0.8501965421659426
8	0.16072636743845573	0.734265802411928
9	0.1306249735146299	0.6713436885716245
>10	0.9651841951448202	11.351425806145166
>50	0.144727441536377	5.766294362406983
>100	0.10966725626279157	11.563250233254173
>500	0.004073820595861843	1.4248527889636584
>1k	8.147641191723687E-4	1.410429318333475
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	4417	0.4093282734909669	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	4199	0.38912597246741454	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	3415	0.3164718256671161	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	2081	0.1928485707798737	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	9.26711056126255E-5	9.26711056126255E-5
2	0.0	0.0	0.0	9.267110561262551E-4	9.26711056126255E-5
3	0.0	0.0	0.0	0.0016680799010272591	9.26711056126255E-5
4	0.0	0.0	0.0	0.003243488696441893	9.26711056126255E-5
5	0.0	9.26711056126255E-5	0.0	0.0035215020132797694	9.26711056126255E-5
6	0.0	9.26711056126255E-5	0.0	0.0035215020132797694	9.26711056126255E-5
7	0.0	9.26711056126255E-5	0.0	0.0038921864357302715	9.26711056126255E-5
8	0.0	9.26711056126255E-5	0.0	0.004355541963793399	9.26711056126255E-5
9	0.0	9.26711056126255E-5	0.0	0.0073210173433974155	1.85342211225251E-4
10	0.0	9.26711056126255E-5	0.0	0.01093519046228981	1.85342211225251E-4
11	0.0	9.26711056126255E-5	0.0	0.012510599257704444	1.85342211225251E-4
12	0.0	9.26711056126255E-5	0.0	0.013437310313830698	1.85342211225251E-4
13	0.0	9.26711056126255E-5	0.0	0.01445669247556958	1.85342211225251E-4
14	0.0	9.26711056126255E-5	0.0	0.01547607463730846	1.85342211225251E-4
15	0.0	9.26711056126255E-5	0.0	0.01631011458782209	1.85342211225251E-4
16	0.0	9.26711056126255E-5	0.0	0.019182918861813482	1.85342211225251E-4
17	0.0	9.26711056126255E-5	0.0	0.022426407558255375	1.85342211225251E-4
18	0.0	9.26711056126255E-5	0.0	0.024835856304183637	1.85342211225251E-4
19	0.0	9.26711056126255E-5	0.0	0.026689278416436146	1.85342211225251E-4
20	0.0	9.26711056126255E-5	0.0	0.02891338495113916	1.85342211225251E-4
21	0.0	9.26711056126255E-5	0.0	0.032898242492482056	1.85342211225251E-4
22	0.0	9.26711056126255E-5	0.0	0.03966323320220372	1.85342211225251E-4
23	0.0	9.26711056126255E-5	0.0	0.04948637039714202	1.85342211225251E-4
24	0.0	9.26711056126255E-5	0.0	0.06440641840077473	1.85342211225251E-4
25	0.0	9.26711056126255E-5	0.0	0.06922531589263126	1.85342211225251E-4
26	0.0	9.26711056126255E-5	0.0	0.07617564881357818	1.85342211225251E-4
27	0.0	9.26711056126255E-5	0.0	0.08516474605800284	1.85342211225251E-4
28	0.0	9.26711056126255E-5	0.0	0.10814718024993397	1.85342211225251E-4
29	0.0	9.26711056126255E-5	0.0	0.14289884485466853	1.85342211225251E-4
30	0.0	9.26711056126255E-5	0.0	0.1918291886181348	1.85342211225251E-4
31	0.0	9.26711056126255E-5	0.0	0.37290852898520505	1.85342211225251E-4
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
AACTCCG	45	0.0038256592	20.555555	7
CTATCGC	55	5.142814E-4	20.181818	32
AAGACGG	225	0.0	18.911112	5
CGGATTC	50	0.007034609	18.5	16
CGAATTA	110	3.8509825E-8	18.5	15
TATCGCC	60	9.236315E-4	18.5	33
TCTATCG	60	9.236315E-4	18.5	31
GGTATCA	2200	0.0	17.490908	1
TTATACC	75	2.0671188E-4	17.266666	4
GACGGAC	250	0.0	17.02	7
TCTAATA	175	7.2759576E-12	16.914286	2
TACCGTC	110	7.806502E-7	16.818182	7
CGCAAGA	265	0.0	16.754715	2
TAATACT	170	8.54925E-11	16.32353	4
ATACCGT	125	1.6573176E-7	16.28	6
AATACTG	195	1.8189894E-12	16.128206	5
CACACTA	115	1.2419459E-6	16.086956	10
GCGCAAG	265	0.0	16.056602	1
CGCCTAT	70	0.0025923564	15.857142	36
GTAGCAC	70	0.0025923564	15.857142	3
>>END_MODULE