FastQCFastQC Report
Fri 10 Feb 2017
ERR1630597.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1630597.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1357836
Sequences flagged as poor quality0
Sequence length43
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT161381.1885087742555065No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT135831.0003417202077423No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT108350.7979608730362134No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT69270.5101499739291049No Hit
CTTATACACATCTCCGAGCCCACGAGACTCGACGTCATCTCGT27040.19914039692569646No Hit
GTGTAGCGCGCGTGCAGCCCCGGACATCTAAGGGCATCACAGA23590.1737323211345111No Hit
GGGTAGGCACACGCTGAGCCAGTCAGTGTAGCGCGCGTGCAGC20790.153111274115578No Hit
CTTCTTAGAGGGACAAGTGGCGTTCAGCCACCCGAGATTGAGC18050.13293210667562208No Hit
TGCCTACCCTACGCCGGCAGGCGCGGGTAACCCGTTGAACCCC17820.13123823495620973No Hit
TCTTAGAGGGACAAGTGGCGTTCAGCCACCCGAGATTGAGCAA16810.1237999287100946No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT16730.12321075593812508No Hit
GGGTTCAACGGGTTACCCGCGCCTGCCGGCGTAGGGTAGGCAC15900.11709808842894134No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT14940.11002801516530715No Hit
GTACATGGGGAATAATTGCAATCCCCGATCCCCATCACGAATG14050.10347346807714629No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT13940.1026633555156882No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCTCTAC450.0038261620.5555551
CTAAGCG555.143753E-420.1818183
GCCGTCT2150.019.79069736
CTTTGCG759.2678E-619.7333349
CCGTCTT2300.018.537
CCAAACG801.6170217E-518.59
CGCAATT500.00703552418.49999812
TTAGACT1351.1532393E-917.8148144
GGTATCA67500.017.6777781
GGACTGA1054.7988397E-717.6190476
ATTAGAC953.6073616E-617.5263163
CAATACG752.0676141E-417.2666664
GGTATAC803.3831294E-416.18753
AAGGGCG803.3831294E-416.18755
TAGTACT803.3831294E-416.18754
GATCGTT700.002592817615.85714311
AGTACGT700.002592817615.85714323
GTACGTG700.002592817615.85714324
TCTTATA25400.015.80511937
TATGCCG2700.015.75925833
Produced by FastQC (version 0.11.5)