##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1630597.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1357836
Sequences flagged as poor quality	0
Sequence length	43
%GC	46
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.061035353312185	33.0	31.0	34.0	30.0	34.0
2	32.219296733920736	33.0	31.0	34.0	30.0	34.0
3	32.308643311857985	34.0	31.0	34.0	30.0	34.0
4	35.902683387389935	37.0	35.0	37.0	35.0	37.0
5	35.87917833965221	37.0	35.0	37.0	35.0	37.0
6	35.98353703981924	37.0	35.0	37.0	35.0	37.0
7	35.930294232882325	37.0	35.0	37.0	35.0	37.0
8	35.94297912266283	37.0	35.0	37.0	35.0	37.0
9	37.63651280419727	39.0	37.0	39.0	35.0	39.0
10	37.55828612586498	39.0	37.0	39.0	35.0	39.0
11	37.67433401382789	39.0	37.0	39.0	35.0	39.0
12	37.62812445685635	39.0	37.0	39.0	35.0	39.0
13	37.670083868744086	39.0	37.0	39.0	35.0	39.0
14	38.89923378081005	40.0	38.0	41.0	36.0	41.0
15	38.90118983441299	40.0	38.0	41.0	36.0	41.0
16	38.84691671159109	40.0	38.0	41.0	35.0	41.0
17	38.88262131803841	40.0	38.0	41.0	36.0	41.0
18	38.91039050371326	40.0	38.0	41.0	36.0	41.0
19	39.00271166768299	40.0	38.0	41.0	36.0	41.0
20	38.974600761800396	40.0	38.0	41.0	35.0	41.0
21	38.93098798382132	40.0	38.0	41.0	35.0	41.0
22	38.82616678302829	40.0	38.0	41.0	35.0	41.0
23	38.75729174951909	40.0	38.0	41.0	35.0	41.0
24	38.71393820755968	40.0	38.0	41.0	35.0	41.0
25	38.641309407027066	40.0	38.0	41.0	35.0	41.0
26	38.498158835087594	40.0	38.0	41.0	34.0	41.0
27	38.33257330045749	40.0	38.0	41.0	34.0	41.0
28	38.227759464324116	40.0	38.0	41.0	34.0	41.0
29	38.13009892210842	40.0	37.0	41.0	34.0	41.0
30	37.99828845309743	40.0	37.0	41.0	33.0	41.0
31	37.897236485113076	40.0	37.0	41.0	33.0	41.0
32	37.726684960481236	40.0	37.0	41.0	33.0	41.0
33	37.60129573821875	40.0	36.0	41.0	33.0	41.0
34	37.49600688153798	40.0	36.0	41.0	33.0	41.0
35	37.36291054295217	40.0	36.0	41.0	33.0	41.0
36	37.28332729431242	40.0	36.0	41.0	33.0	41.0
37	37.09126212591211	40.0	35.0	41.0	32.0	41.0
38	36.88991454048943	40.0	35.0	41.0	31.0	41.0
39	36.75667827337028	39.0	35.0	41.0	31.0	41.0
40	36.51562854424245	39.0	35.0	41.0	30.0	41.0
41	36.3905898797793	39.0	35.0	41.0	30.0	41.0
42	36.16440866201809	39.0	35.0	41.0	29.0	41.0
43	35.35869943056451	38.0	35.0	40.0	26.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
9	3.0
10	2.0
11	7.0
12	7.0
13	7.0
14	6.0
15	1.0
16	8.0
17	15.0
18	36.0
19	70.0
20	149.0
21	345.0
22	687.0
23	1115.0
24	1821.0
25	2997.0
26	4900.0
27	7531.0
28	11195.0
29	15714.0
30	20800.0
31	25963.0
32	33275.0
33	42866.0
34	57561.0
35	75889.0
36	108100.0
37	179896.0
38	298883.0
39	467987.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	45.46940867674741	19.40978144636024	12.656609487449147	22.464200389443203
2	19.275229114561697	21.382773766493155	35.30853505136113	24.033462067584008
3	21.14165480956463	24.9304039663111	29.89499468271573	24.032946541408535
4	13.155344238921343	15.373285139000586	34.7545653525168	36.71680526956127
5	13.06652644354694	39.73432726779965	33.53976474331215	13.659381545341265
6	33.05877882159554	34.61294294745463	16.717777404635022	15.610500826314814
7	27.704818549515554	31.93773032973054	21.33902768817442	19.018423432579485
8	29.616905134346123	31.144630132063078	19.971852270819156	19.266612462771647
9	27.545300021504804	11.920659048662726	21.443826794988496	39.09021413484397
10	17.950989662963714	28.046317817468385	32.25382152189219	21.748870997675713
11	33.69751575300699	22.21063515770682	21.177152469075793	22.914696620210393
12	21.95670169298796	28.550355123888306	28.112231521332475	21.380711661791263
13	30.806813193935056	19.906527739727036	26.76147929499586	22.525179771342046
14	22.5975743756978	21.867073785052096	27.837824302787674	27.69752753646243
15	26.622950046986528	27.17986560969071	25.09721350737497	21.099970835947786
16	23.94626449733252	26.049832233053184	26.349132001213693	23.6547712684006
17	21.887400245685047	27.123010437195656	27.32804256184105	23.661546755278252
18	21.64591305577404	23.943834159648144	30.438801151243595	23.97145163333422
19	22.14008171826347	25.485552010699376	30.266099882460036	22.108266388577118
20	23.78085424160208	23.753384061109	30.87582005485198	21.589941642436937
21	24.463926424104237	24.922229194100023	28.99783184419915	21.61601253759659
22	24.294023725987525	25.05066885838938	29.04017863718446	21.615128778438635
23	23.062578985974742	24.657764266082207	29.23040779593412	23.049248952008934
24	22.25688522030643	25.9837712359961	29.290798005060992	22.468545538636477
25	22.179850880371415	25.423320636660097	29.62964599553996	22.767182487428524
26	22.5066944756215	25.85960307430352	29.126124215295512	22.50757823477946
27	22.55412288376505	25.782642380965004	29.435734506965495	22.22750022830445
28	21.80940849999558	25.46898152648773	29.911270580541387	22.810339392975294
29	21.390948538704233	25.996438450593445	30.924353161942975	21.68825984875935
30	20.610441909037615	26.996485584415204	30.28561623053152	22.10745627601566
31	22.60501268194392	27.212564698535026	28.415213619317797	21.767209000203263
32	20.54865241457731	26.727233627625136	29.846608868817736	22.87750508897982
33	20.688360008130584	25.955122709959085	30.21152775445636	23.144989527453976
34	22.050159223941627	25.40240500325518	30.375244138467384	22.17219163433581
35	20.770696903013324	26.289920137630762	30.48048512486044	22.458897834495474
36	21.173470139250984	27.05879060505098	29.020514995919978	22.747224259778058
37	21.51850444383563	25.912186744201804	29.804262075832426	22.765046736130138
38	21.087450914543435	25.52973996859709	29.799548693656668	23.583260423202802
39	21.10144376787771	25.909314526938452	30.479380425912993	22.50986127927084
40	19.96286738604662	25.611414044111363	30.872358664816662	23.55335990502535
41	20.193307586483197	25.41190541420319	30.876556520816944	23.518230478496665
42	19.8280204678621	26.03186246350811	31.33633222274266	22.803784845887133
43	19.29297794431728	24.737228943701595	31.02392336040582	24.9458697515753
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	63.0
1	145.5
2	228.0
3	1157.0
4	2086.0
5	2086.0
6	3168.5
7	4251.0
8	4298.0
9	4345.0
10	5836.0
11	7327.0
12	7327.0
13	12425.0
14	17523.0
15	25152.0
16	32781.0
17	29260.5
18	25740.0
19	25740.0
20	27274.5
21	28809.0
22	22777.0
23	16745.0
24	17952.5
25	19160.0
26	19160.0
27	21126.0
28	23092.0
29	26674.5
30	30257.0
31	34329.0
32	38401.0
33	38401.0
34	44015.0
35	49629.0
36	53975.0
37	58321.0
38	65016.5
39	71712.0
40	71712.0
41	74659.5
42	77607.0
43	80686.5
44	83766.0
45	84188.5
46	84611.0
47	84611.0
48	88243.0
49	91875.0
50	91300.0
51	90725.0
52	93496.0
53	96267.0
54	96267.0
55	90808.5
56	85350.0
57	80780.5
58	76211.0
59	71025.0
60	65839.0
61	65839.0
62	58986.0
63	52133.0
64	46120.5
65	40108.0
66	36087.5
67	32067.0
68	32067.0
69	25909.5
70	19752.0
71	16840.5
72	13929.0
73	10575.0
74	7221.0
75	7221.0
76	5566.5
77	3912.0
78	2957.0
79	2002.0
80	1613.0
81	1224.0
82	1224.0
83	1119.0
84	1014.0
85	944.5
86	875.0
87	686.0
88	497.0
89	497.0
90	399.5
91	302.0
92	184.5
93	67.0
94	38.5
95	10.0
96	10.0
97	6.0
98	2.0
99	1.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	1357836.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	49.82619385926892
#Duplication Level	Percentage of deduplicated	Percentage of total
1	81.55599279858782	40.636247075675776
2	11.25921405258138	11.220075641738493
3	3.0969224811543747	4.62923579739378
4	1.1687132268253482	2.3293012722276614
5	0.590748208778724	1.4717367386312288
6	0.37174676600219053	1.1113635857628856
7	0.2601249878932114	0.9072726652084994
8	0.19233188831847978	0.7666532762140666
9	0.14767109440758602	0.6622099719626504
>10	1.0951031208331525	11.021225526518421
>50	0.14210182689464212	4.967579893744588
>100	0.10332012602199014	10.14372115750302
>500	0.01230353704808462	4.295848277941062
>1k	0.0031129431085515306	2.330432324638017
>5k	1.4823538612150145E-4	0.5116285722859896
>10k+	4.447061583645043E-4	2.995468222553861
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	16138	1.1885087742555065	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	13583	1.0003417202077423	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	10835	0.7979608730362134	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	6927	0.5101499739291049	No Hit
CTTATACACATCTCCGAGCCCACGAGACTCGACGTCATCTCGT	2704	0.19914039692569646	No Hit
GTGTAGCGCGCGTGCAGCCCCGGACATCTAAGGGCATCACAGA	2359	0.1737323211345111	No Hit
GGGTAGGCACACGCTGAGCCAGTCAGTGTAGCGCGCGTGCAGC	2079	0.153111274115578	No Hit
CTTCTTAGAGGGACAAGTGGCGTTCAGCCACCCGAGATTGAGC	1805	0.13293210667562208	No Hit
TGCCTACCCTACGCCGGCAGGCGCGGGTAACCCGTTGAACCCC	1782	0.13123823495620973	No Hit
TCTTAGAGGGACAAGTGGCGTTCAGCCACCCGAGATTGAGCAA	1681	0.1237999287100946	No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1673	0.12321075593812508	No Hit
GGGTTCAACGGGTTACCCGCGCCTGCCGGCGTAGGGTAGGCAC	1590	0.11709808842894134	No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1494	0.11002801516530715	No Hit
GTACATGGGGAATAATTGCAATCCCCGATCCCCATCACGAATG	1405	0.10347346807714629	No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1394	0.1026633555156882	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	1.4729319299237905E-4	0.0
2	0.0	0.0	0.0	0.0013256387369314114	0.0
3	0.0	0.0	0.0	0.0017675183159085487	0.0
4	0.0	0.0	0.0	0.002503984280870444	0.0
5	0.0	0.0	0.0	0.0028722172633513913	0.0
6	0.0	0.0	0.0	0.002945863859847581	0.0
7	0.0	0.0	0.0	0.0033877434388247182	0.0
8	0.0	0.0	0.0	0.0035350366318170974	0.0
9	0.0	0.0	0.0	0.005744434526702783	0.0
10	0.0	0.0	0.0	0.007953832421588468	0.0
11	0.0	0.0	0.0	0.008984884772535121	0.0
12	0.0	0.0	0.0	0.01097334287793224	0.0
13	0.0	0.0	0.0	0.011709808842894135	0.0
14	0.0	0.0	0.0	0.012888154386833167	0.0
15	0.0	0.0	0.0	0.015613078457192179	0.0
16	0.0	0.0	0.0	0.023198677896299702	0.0
17	0.0	0.0	0.0	0.03439296056372051	0.0
18	0.0	0.0	0.0	0.03719153123057571	0.0
19	0.0	0.0	0.0	0.04470348407318704	0.0
20	0.0	0.0	0.0	0.04904863326646222	0.0
21	0.0	0.0	0.0	0.0618631410567992	0.0
22	0.0	0.0	0.0	0.0879340362164503	0.0
23	0.0	0.0	0.0	0.13109094176321737	0.0
24	0.0	0.0	0.0	0.19877216394321554	0.0
25	0.0	0.0	0.0	0.21062926597910203	0.0
26	0.0	0.0	0.0	0.23530087580532552	0.0
27	0.0	0.0	0.0	0.2565847421927243	0.0
28	0.0	0.0	0.0	0.30357127075729323	0.0
29	0.0	0.0	0.0	0.37986914472734556	0.0
30	0.0	0.0	0.0	0.5169991074032505	0.0
31	0.0	0.0	0.0	0.9875272124174054	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GCTCTAC	45	0.00382616	20.555555	1
CTAAGCG	55	5.143753E-4	20.181818	3
GCCGTCT	215	0.0	19.790697	36
CTTTGCG	75	9.2678E-6	19.733334	9
CCGTCTT	230	0.0	18.5	37
CCAAACG	80	1.6170217E-5	18.5	9
CGCAATT	50	0.007035524	18.499998	12
TTAGACT	135	1.1532393E-9	17.814814	4
GGTATCA	6750	0.0	17.677778	1
GGACTGA	105	4.7988397E-7	17.619047	6
ATTAGAC	95	3.6073616E-6	17.526316	3
CAATACG	75	2.0676141E-4	17.266666	4
GGTATAC	80	3.3831294E-4	16.1875	3
AAGGGCG	80	3.3831294E-4	16.1875	5
TAGTACT	80	3.3831294E-4	16.1875	4
GATCGTT	70	0.0025928176	15.857143	11
AGTACGT	70	0.0025928176	15.857143	23
GTACGTG	70	0.0025928176	15.857143	24
TCTTATA	2540	0.0	15.805119	37
TATGCCG	270	0.0	15.759258	33
>>END_MODULE