FastQCFastQC Report
Fri 10 Feb 2017
ERR1630596.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1630596.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences391979
Sequences flagged as poor quality0
Sequence length43
%GC34

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT182814.663770252998247No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT168914.309159419254603No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT120743.080267055122851No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT86402.2041997147806387No Hit
CTTATACACATCTCCGAGCCCACGAGACATCTCAGGATCTCGT27460.7005477334244946No Hit
AACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT21280.542886226047824No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT20860.5321713663231958No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT20680.5275792835840696No Hit
TCTCCGAGCCCACGAGACATCTCAGGATCTCGTATGCCGTCTT16870.4303801989392289TruSeq Adapter, Index 1 (95% over 22bp)
GAACAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAA16720.42655346332329025No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTG14580.3719587018692328No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT13210.33700784991032684No Hit
ATACACATCTCCGAGCCCACGAGACATCTCAGGATCTCGTATG11600.2959342209659191No Hit
ACACATCTCCGAGCCCACGAGACATCTCAGGATCTCGTATGCC11260.2872602869031249No Hit
GTACATGGGAATGGTATCAACGCAAAAAAAAAAAAAAAAAAAA10540.2688919559466196No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTT10540.2688919559466196No Hit
TCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTT9780.24950316215919732No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGG9060.231134831202692No Hit
ACGCAGAGTACATGGGAAAAAAAAAAAAAAAAAAAAAAAAAAA8830.22526716992491946No Hit
GAATGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAA8450.2155727730312083No Hit
CATCTCCGAGCCCACGAGACATCTCAGGATCTCGTATGCCGTC8420.2148074259080206No Hit
GAACAGTGGTATCAACGCAGAAAAAAAAAAAAAAAAAAAAAAA8080.20613349184522642No Hit
GGTATCAACGCAGAGTACATGGGAAAAAAAAAAAAAAAAAAAA7760.19796978919789068No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT7360.18776516088872108No Hit
CAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT7360.18776516088872108No Hit
TATCAACGCAGAGTACATGGGAAAAAAAAAAAAAAAAAAAAAA7290.1859793509346164No Hit
GTACATGGGAACAGTGGTATCAACGCAAAAAAAAAAAAAAAAA7250.18495888810369943No Hit
GTACATGGGAAGCAGTGGTATCAAAAAAAAAAAAAAAAAAAAA7100.18113215248776082No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGT6520.16633544143946488No Hit
CTCTATGGGCAGTCGGTGATTCCTCGCCTTATTGATATGCCCA6190.1579166230843999No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTC5940.1515387303911689No Hit
GTACATGGGAAGCAGAGGTATCAACGCAAAAAAAAAAAAAAAA5660.14439549057475018No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTCC5330.13597667221968524No Hit
GTATCAACGCAGAGTACATGGGAAAAAAAAAAAAAAAAAAAAA4840.12347600254095244No Hit
GTACATGGGAATGGTATCAACGCAGAAAAAAAAAAAAAAAAAA4530.115567415601346No Hit
GTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA4430.11301625852405359No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGTT4150.10587301870763485No Hit
GAGTACATGGGAATGGTATCAACGCAAAAAAAAAAAAAAAAAA4120.10510767158444713No Hit
GGTATCAACGCAGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA4110.10485255587671788No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTCCC4100.10459744016898866No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGG4000.10204628309169624No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTG3980.10153605167623775No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAACCCT250.00549197929.635
GCTCAGG250.00549197929.61
AGCGCAA358.858929E-426.4285718
TTTGAGC358.858929E-426.428573
GCCGTCT2900.026.15517236
ACACTGC509.071142E-625.9000026
CGTCTTC659.3588824E-825.61538537
CCGTCTT3000.025.28333337
TGGTACT451.3210397E-424.66666627
TTAGAGC400.001928929423.1250023
ACAGAGG603.718227E-521.5833323
TTTAGAG450.003821378620.5555552
CTATACC450.003821378620.5555554
TTTTGAG450.003821378620.5555552
GGACAAA450.003821378620.55555511
GGTACTG555.134788E-420.18181828
AAAACCC555.134788E-420.18181837
CATCAAC656.887307E-519.9230773
GCATCAA656.887307E-519.9230772
AGTGGCT1254.0927262E-1019.2427
Produced by FastQC (version 0.11.5)