##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1630596.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	391979
Sequences flagged as poor quality	0
Sequence length	43
%GC	34
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.699570640263893	31.0	31.0	34.0	30.0	34.0
2	31.705777605432946	31.0	31.0	34.0	30.0	34.0
3	31.63880973215402	31.0	31.0	34.0	28.0	34.0
4	35.42579066735718	37.0	35.0	37.0	33.0	37.0
5	35.47147423714026	37.0	35.0	37.0	33.0	37.0
6	35.637812229736795	37.0	35.0	37.0	33.0	37.0
7	35.49665415749313	37.0	35.0	37.0	33.0	37.0
8	35.654285050984875	37.0	35.0	37.0	33.0	37.0
9	37.10533217340725	39.0	37.0	39.0	33.0	39.0
10	37.03980315271992	39.0	37.0	39.0	33.0	39.0
11	37.252102791220956	39.0	37.0	39.0	34.0	39.0
12	37.23403549679957	39.0	37.0	39.0	34.0	39.0
13	37.31822112919315	39.0	37.0	39.0	34.0	39.0
14	38.231489442036434	40.0	38.0	41.0	33.0	41.0
15	38.16366693113662	40.0	37.0	41.0	33.0	41.0
16	37.989430556228776	40.0	37.0	41.0	33.0	41.0
17	38.249592452656906	40.0	38.0	41.0	34.0	41.0
18	38.41185369624393	40.0	38.0	41.0	34.0	41.0
19	38.57613545623617	40.0	38.0	41.0	34.0	41.0
20	38.61372675577008	40.0	38.0	41.0	34.0	41.0
21	38.57986014556902	40.0	38.0	41.0	34.0	41.0
22	38.42553300049237	40.0	38.0	41.0	34.0	41.0
23	38.2695118871164	40.0	37.0	41.0	34.0	41.0
24	38.1326831284329	40.0	37.0	41.0	33.0	41.0
25	38.00426808579031	40.0	37.0	41.0	33.0	41.0
26	37.594771658685794	39.0	36.0	41.0	33.0	41.0
27	37.22972404133895	39.0	35.0	41.0	33.0	41.0
28	36.997474354493484	39.0	35.0	41.0	32.0	41.0
29	36.672462045160586	38.0	35.0	40.0	32.0	41.0
30	36.26128950785629	38.0	35.0	40.0	31.0	41.0
31	35.77715642929851	37.0	35.0	40.0	31.0	41.0
32	35.291847267328095	36.0	35.0	40.0	30.0	41.0
33	34.82372780174448	35.0	34.0	40.0	28.0	41.0
34	34.45047821439414	35.0	34.0	40.0	25.0	41.0
35	34.02596057441853	35.0	33.0	40.0	22.0	41.0
36	33.56614257396442	35.0	33.0	40.0	21.0	41.0
37	32.95008150946862	35.0	33.0	40.0	16.0	41.0
38	32.32325456210664	35.0	33.0	39.0	12.0	41.0
39	31.716122547381364	35.0	32.0	39.0	10.0	41.0
40	30.943468910324277	35.0	30.0	39.0	8.0	41.0
41	30.277915398529004	35.0	26.0	39.0	7.0	41.0
42	29.514894930595773	35.0	22.0	38.0	7.0	41.0
43	28.571512759612123	35.0	20.0	38.0	7.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	3.0
9	3.0
10	7.0
11	8.0
12	14.0
13	5.0
14	3.0
15	6.0
16	5.0
17	12.0
18	30.0
19	89.0
20	208.0
21	392.0
22	659.0
23	1181.0
24	1889.0
25	2957.0
26	4363.0
27	6239.0
28	8607.0
29	11298.0
30	13289.0
31	15273.0
32	18201.0
33	21605.0
34	26384.0
35	31039.0
36	43257.0
37	73753.0
38	66873.0
39	44327.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	51.88160590235701	19.157148724804134	15.153107691993704	13.808137680845148
2	16.250870582352626	27.568568724344928	32.68006704440799	23.500493648894455
3	24.707701177869225	31.324892404950266	30.908288454228416	13.059117962952097
4	13.089731847879607	18.02290428823993	27.561170368820783	41.326193495059684
5	9.636485628056606	47.62627589743328	28.321925409269372	14.41531306524074
6	28.66607649899612	29.19365578258019	20.319200773510826	21.821066944912864
7	21.534827120840657	38.55487156199695	22.2925207727965	17.617780544365896
8	36.38893920337569	27.821388390704605	20.174805282936077	15.61486712298363
9	28.8377693702979	9.782922044293189	24.868424073738645	36.51088451167027
10	20.258738350778994	34.62353850588934	24.842147155842532	20.275575987489127
11	24.976338018108112	26.82184504782144	19.542373443475288	28.65944349059516
12	18.95127034866664	36.48129108957367	29.28651790019363	15.280920661566052
13	31.219274501950363	19.85182879695086	32.792062840101124	16.136833860997655
14	22.566004811482248	24.58779679523648	35.652930386576834	17.19326800670444
15	27.363455695330618	24.825054403424673	32.53822271091054	15.273267190334176
16	17.440730243201806	25.164358294704563	37.21117712938703	20.1837343327066
17	14.652315557721204	32.81910510512043	35.32740274351432	17.201176593644046
18	13.695886769444282	23.241296089841548	41.51421377165613	21.548603369058036
19	14.3844440646055	28.976552315302605	44.570755065960164	12.068248554131726
20	15.800846473918245	23.78112092739662	46.39559772334743	14.022434875337709
21	19.375527770620366	24.180887241408342	42.74642263998837	13.697162347982928
22	17.419300523752547	26.33738031884361	42.10531686646479	14.138002290939056
23	15.305156653800331	27.404019092859567	41.65376206378403	15.637062189556072
24	13.621137867079614	30.004158386035982	43.080879332821404	13.293824414063
25	13.29637557114029	29.15589865783626	42.60381295936772	14.943912811655727
26	15.29469690978343	28.935478686358195	41.96704415287554	13.802780250982833
27	12.834616140150365	30.994262447733167	42.391301574829264	13.779819837287203
28	12.76292862627845	30.232997175869116	43.4926360850964	13.511438112756041
29	12.321578451906863	31.968294219843408	43.52631135851666	12.183815969733072
30	11.757262506409782	32.18131583579733	44.24369672865128	11.817724929141612
31	11.789151969875936	32.88109822209863	41.708867056653546	13.620882751371886
32	10.837825495753599	33.807933588278964	43.33267853635016	12.021562379617276
33	11.050336880292058	33.19591100543652	42.872960031022075	12.880792083249357
34	11.568476882690144	35.01896785286967	40.06923840307772	13.343316861362469
35	11.784815002844539	34.11789917316999	40.83560598909636	13.261679834889115
36	11.888391980182613	34.23295635735588	38.83779488186867	15.040856780592836
37	11.39499820143426	34.93733082639631	39.28552294893349	14.382148023235938
38	11.682768719752843	33.11605978891726	39.77713091772774	15.424040573602158
39	12.407042213996158	33.404340538651304	39.2934315358731	14.895185711479442
40	11.679197099844634	33.42194352248462	39.88453463068174	15.014324746988997
41	11.468726640968011	34.49623576773245	37.860701721265684	16.174335870033854
42	11.811091920740651	33.76124741376451	38.303327474175916	16.124333191318925
43	10.972781705142367	34.510267131657564	37.445628464790204	17.071322698409862
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	37.0
1	118.0
2	199.0
3	910.5
4	1622.0
5	1622.0
6	2781.5
7	3941.0
8	4315.5
9	4690.0
10	6609.0
11	8528.0
12	8528.0
13	15479.5
14	22431.0
15	32302.5
16	42174.0
17	37101.5
18	32029.0
19	32029.0
20	33596.0
21	35163.0
22	25075.0
23	14987.0
24	13020.0
25	11053.0
26	11053.0
27	11448.0
28	11843.0
29	11502.5
30	11162.0
31	11118.5
32	11075.0
33	11075.0
34	10066.5
35	9058.0
36	8629.0
37	8200.0
38	8879.0
39	9558.0
40	9558.0
41	10864.5
42	12171.0
43	12974.5
44	13778.0
45	16734.5
46	19691.0
47	19691.0
48	21835.0
49	23979.0
50	22336.5
51	20694.0
52	15310.5
53	9927.0
54	9927.0
55	10263.0
56	10599.0
57	9579.5
58	8560.0
59	7526.0
60	6492.0
61	6492.0
62	6277.0
63	6062.0
64	5829.0
65	5596.0
66	5296.0
67	4996.0
68	4996.0
69	4455.5
70	3915.0
71	3426.5
72	2938.0
73	2504.5
74	2071.0
75	2071.0
76	1700.0
77	1329.0
78	1045.5
79	762.0
80	583.0
81	404.0
82	404.0
83	289.5
84	175.0
85	119.5
86	64.0
87	43.5
88	23.0
89	23.0
90	13.0
91	3.0
92	1.5
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	391979.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	36.73894704372298
#Duplication Level	Percentage of deduplicated	Percentage of total
1	79.68834232686352	29.27665788748708
2	10.184628103902938	7.483450251386055
3	3.471467316358133	3.8261416169908977
4	1.6897084198207628	2.4831243262051133
5	1.0019845661149551	1.8405928956562543
6	0.6620506092069256	1.4593825363150668
7	0.45649689356451406	1.1739850638803506
8	0.3449736485477821	1.0139174884381497
9	0.27258813981281393	0.9013141109996934
>10	1.8828902432172532	13.424278018498823
>50	0.18912211142054397	4.8624258324456635
>100	0.13280265912251432	9.753972576102193
>500	0.011820131963783998	3.2283339989728557
>1k	0.008343622562671058	4.996535291969382
>5k	6.95301880222588E-4	2.207058533876046
>10k+	0.0020859056406677644	12.068829570776352
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	18281	4.663770252998247	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	16891	4.309159419254603	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	12074	3.080267055122851	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	8640	2.2041997147806387	No Hit
CTTATACACATCTCCGAGCCCACGAGACATCTCAGGATCTCGT	2746	0.7005477334244946	No Hit
AACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	2128	0.542886226047824	No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT	2086	0.5321713663231958	No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	2068	0.5275792835840696	No Hit
TCTCCGAGCCCACGAGACATCTCAGGATCTCGTATGCCGTCTT	1687	0.4303801989392289	TruSeq Adapter, Index 1 (95% over 22bp)
GAACAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAA	1672	0.42655346332329025	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTG	1458	0.3719587018692328	No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1321	0.33700784991032684	No Hit
ATACACATCTCCGAGCCCACGAGACATCTCAGGATCTCGTATG	1160	0.2959342209659191	No Hit
ACACATCTCCGAGCCCACGAGACATCTCAGGATCTCGTATGCC	1126	0.2872602869031249	No Hit
GTACATGGGAATGGTATCAACGCAAAAAAAAAAAAAAAAAAAA	1054	0.2688919559466196	No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTT	1054	0.2688919559466196	No Hit
TCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	978	0.24950316215919732	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGG	906	0.231134831202692	No Hit
ACGCAGAGTACATGGGAAAAAAAAAAAAAAAAAAAAAAAAAAA	883	0.22526716992491946	No Hit
GAATGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAA	845	0.2155727730312083	No Hit
CATCTCCGAGCCCACGAGACATCTCAGGATCTCGTATGCCGTC	842	0.2148074259080206	No Hit
GAACAGTGGTATCAACGCAGAAAAAAAAAAAAAAAAAAAAAAA	808	0.20613349184522642	No Hit
GGTATCAACGCAGAGTACATGGGAAAAAAAAAAAAAAAAAAAA	776	0.19796978919789068	No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	736	0.18776516088872108	No Hit
CAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	736	0.18776516088872108	No Hit
TATCAACGCAGAGTACATGGGAAAAAAAAAAAAAAAAAAAAAA	729	0.1859793509346164	No Hit
GTACATGGGAACAGTGGTATCAACGCAAAAAAAAAAAAAAAAA	725	0.18495888810369943	No Hit
GTACATGGGAAGCAGTGGTATCAAAAAAAAAAAAAAAAAAAAA	710	0.18113215248776082	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGT	652	0.16633544143946488	No Hit
CTCTATGGGCAGTCGGTGATTCCTCGCCTTATTGATATGCCCA	619	0.1579166230843999	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTC	594	0.1515387303911689	No Hit
GTACATGGGAAGCAGAGGTATCAACGCAAAAAAAAAAAAAAAA	566	0.14439549057475018	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTCC	533	0.13597667221968524	No Hit
GTATCAACGCAGAGTACATGGGAAAAAAAAAAAAAAAAAAAAA	484	0.12347600254095244	No Hit
GTACATGGGAATGGTATCAACGCAGAAAAAAAAAAAAAAAAAA	453	0.115567415601346	No Hit
GTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	443	0.11301625852405359	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGTT	415	0.10587301870763485	No Hit
GAGTACATGGGAATGGTATCAACGCAAAAAAAAAAAAAAAAAA	412	0.10510767158444713	No Hit
GGTATCAACGCAGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	411	0.10485255587671788	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTCCC	410	0.10459744016898866	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGG	400	0.10204628309169624	No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTG	398	0.10153605167623775	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	5.102314154584812E-4	0.0
3	0.0	0.0	0.0	0.0015306942463754437	0.0
4	0.0	0.0	0.0	0.002296041369563165	0.0
5	0.0	0.0	0.0	0.002551157077292406	0.0
6	0.0	0.0	0.0	0.002551157077292406	0.0
7	0.0	0.0	0.0	0.0030613884927508874	0.0
8	0.0	0.0	0.0	0.0030613884927508874	0.0
9	0.0	0.0	0.0	0.00433696703139709	0.0
10	0.0	0.0	0.0	0.006633008400960256	0.0
11	0.0	0.0	0.0	0.010204628309169624	0.0
12	0.0	0.0	0.0	0.013266016801920511	0.0
13	0.0	0.0	0.0	0.014796711048295955	0.0
14	0.0	0.0	0.0	0.02040925661833925	0.0
15	0.0	0.0	0.0	0.02984853780432115	0.0
16	0.0	0.0	0.0	0.049747563007201914	0.0
17	0.0	0.0	0.0	0.08597399350475408	0.0
18	0.0	0.0	0.0	0.09949512601440383	0.0
19	0.0	0.0	0.0	0.13240505231147587	0.0
20	0.0	0.0	0.0	0.14898757331387652	0.0
21	0.0	0.0	0.0	0.1915918965046597	0.0
22	0.0	0.0	0.0	0.2739942701012044	0.0
23	0.0	0.0	0.0	0.38165309876294395	0.0
24	0.0	0.0	0.0	0.5653364083279971	0.0
25	0.0	0.0	0.0	0.6007974917023616	0.0
26	0.0	0.0	0.0	0.6653417657578595	0.0
27	0.0	0.0	0.0	0.7145790973496029	0.0
28	0.0	0.0	0.0	0.7926445039147505	0.0
29	0.0	0.0	0.0	0.9153551593325152	0.0
30	0.0	0.0	0.0	1.1635827429530663	0.0
31	0.0	0.0	0.0	1.7995862023220632	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TAACCCT	25	0.005491979	29.6	35
GCTCAGG	25	0.005491979	29.6	1
AGCGCAA	35	8.858929E-4	26.42857	18
TTTGAGC	35	8.858929E-4	26.42857	3
GCCGTCT	290	0.0	26.155172	36
ACACTGC	50	9.071142E-6	25.900002	6
CGTCTTC	65	9.3588824E-8	25.615385	37
CCGTCTT	300	0.0	25.283333	37
TGGTACT	45	1.3210397E-4	24.666666	27
TTAGAGC	40	0.0019289294	23.125002	3
ACAGAGG	60	3.718227E-5	21.583332	3
TTTAGAG	45	0.0038213786	20.555555	2
CTATACC	45	0.0038213786	20.555555	4
TTTTGAG	45	0.0038213786	20.555555	2
GGACAAA	45	0.0038213786	20.555555	11
GGTACTG	55	5.134788E-4	20.181818	28
AAAACCC	55	5.134788E-4	20.181818	37
CATCAAC	65	6.887307E-5	19.923077	3
GCATCAA	65	6.887307E-5	19.923077	2
AGTGGCT	125	4.0927262E-10	19.24	27
>>END_MODULE