FastQCFastQC Report
Fri 10 Feb 2017
ERR1630595.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1630595.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2440866
Sequences flagged as poor quality0
Sequence length43
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT75860.3107913338954289No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT68470.2805151941974693No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT59080.24204524132008884No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT39530.16195071749125106No Hit
GCTTAGGACACAGAGCACATCAAAAGTTCCCAAAGAGGGCTTG25000.10242266474275934No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CATACCG752.0684666E-417.2666665
GACCGAT650.001580621717.0769237
ACCGTCC904.4492495E-516.4444458
GGTATCA40500.015.3024691
TAATACT3850.014.8961044
TAGGACA10450.014.693784
TAGCACT4250.014.3647064
GCTTAGG9900.014.2020211
GACGGAC2750.014.1272747
TCTAGCG2252.7284841E-1113.977777528
TTAGGAC11000.013.9590913
GTAGAAC7850.013.9044593
CGGCATA800.006301296413.87536
CTAATAC4550.013.8241753
GTCGTTG2950.013.796611
ATCTCGC5650.013.75221311
TAGAACA8250.013.6787884
CTTAGGA10800.013.5324082
CGAGCGT1102.458133E-413.4545456
TAGACTG2102.044544E-913.2142855
Produced by FastQC (version 0.11.5)