##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1630595.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	2440866
Sequences flagged as poor quality	0
Sequence length	43
%GC	48
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.07711648242878	33.0	31.0	34.0	30.0	34.0
2	32.25304338706017	34.0	31.0	34.0	30.0	34.0
3	32.3527932299438	34.0	31.0	34.0	30.0	34.0
4	35.930884366450265	37.0	35.0	37.0	35.0	37.0
5	35.888061450321324	37.0	35.0	37.0	35.0	37.0
6	35.962420304924564	37.0	35.0	37.0	35.0	37.0
7	35.91379821751788	37.0	35.0	37.0	35.0	37.0
8	35.90704118947947	37.0	35.0	37.0	35.0	37.0
9	37.62925248661745	39.0	37.0	39.0	35.0	39.0
10	37.53874772314416	39.0	37.0	39.0	35.0	39.0
11	37.635245441576885	39.0	37.0	39.0	35.0	39.0
12	37.58604200312512	39.0	37.0	39.0	35.0	39.0
13	37.61540084543764	39.0	37.0	39.0	35.0	39.0
14	38.92638104672686	40.0	38.0	41.0	36.0	41.0
15	38.944808113186056	40.0	38.0	41.0	36.0	41.0
16	38.9005324339804	40.0	38.0	41.0	35.0	41.0
17	38.884437326752064	40.0	38.0	41.0	35.0	41.0
18	38.86460829885787	40.0	38.0	41.0	35.0	41.0
19	38.93271486431455	40.0	38.0	41.0	35.0	41.0
20	38.89870931054798	40.0	38.0	41.0	35.0	41.0
21	38.85506578402911	40.0	38.0	41.0	35.0	41.0
22	38.79551028200647	40.0	38.0	41.0	35.0	41.0
23	38.74615034172298	40.0	38.0	41.0	35.0	41.0
24	38.69921208292467	40.0	38.0	41.0	34.0	41.0
25	38.64713999047879	40.0	38.0	41.0	34.0	41.0
26	38.51937058404681	40.0	38.0	41.0	34.0	41.0
27	38.40033865029871	40.0	38.0	41.0	34.0	41.0
28	38.313121654363655	40.0	38.0	41.0	34.0	41.0
29	38.24537807483082	40.0	38.0	41.0	34.0	41.0
30	38.165813690714685	40.0	38.0	41.0	33.0	41.0
31	38.12402278535569	40.0	38.0	41.0	33.0	41.0
32	38.02809084972301	40.0	37.0	41.0	33.0	41.0
33	37.96638365235945	40.0	37.0	41.0	33.0	41.0
34	37.90931538232742	40.0	37.0	41.0	33.0	41.0
35	37.85193287955996	40.0	37.0	41.0	33.0	41.0
36	37.82138142773917	40.0	37.0	41.0	33.0	41.0
37	37.69512255076682	40.0	37.0	41.0	33.0	41.0
38	37.54994088163791	40.0	37.0	41.0	32.0	41.0
39	37.496960914691755	40.0	37.0	41.0	32.0	41.0
40	37.33910915224351	40.0	36.0	41.0	32.0	41.0
41	37.28457973522512	40.0	36.0	41.0	32.0	41.0
42	37.16485337581006	40.0	36.0	41.0	31.0	41.0
43	36.36690338592942	39.0	35.0	40.0	30.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	1.0
9	6.0
10	3.0
11	6.0
12	5.0
13	2.0
14	2.0
15	8.0
16	8.0
17	30.0
18	65.0
19	145.0
20	264.0
21	577.0
22	1033.0
23	1822.0
24	3016.0
25	4871.0
26	7703.0
27	11216.0
28	16335.0
29	23282.0
30	31857.0
31	42399.0
32	54843.0
33	71504.0
34	95042.0
35	128893.0
36	183009.0
37	287788.0
38	548489.0
39	926642.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	43.906793736321454	18.70188695323709	11.971120086067813	25.420199224373647
2	18.65772230020001	21.024464268009798	35.11515994733017	25.202653484460026
3	20.50841791396988	23.352121746953745	29.251913050532064	26.88754728854431
4	14.738498549285376	15.402279354950252	33.904524050070755	35.95469804569362
5	14.189799849725466	37.32191771281176	34.35497892960941	14.133303507853359
6	35.1882897299565	34.16938906109553	15.784561708836126	14.857759500111845
7	29.142525644586797	30.23607195151229	21.492986505609075	19.128415898291838
8	26.85972929280018	33.782190419302	20.19434905480268	19.163731233095138
9	26.703022615743755	13.850453076899754	19.601116980612616	39.84540732674387
10	16.756266013783634	27.074775919694076	33.08936254591608	23.079595520606212
11	34.99557124397653	21.85162970847232	21.653339429530337	21.499459618020815
12	21.536126931998727	25.526063290651763	29.064274728723333	23.873535048626184
13	29.749113634259317	20.012241556890054	25.71562715855766	24.52301765029297
14	23.13986101654085	21.00688853873994	25.26263219693338	30.590618247785827
15	25.87638977313789	27.450626130234106	22.678672241737154	23.994311854890846
16	25.31658845671987	26.571348037950465	23.979440083970196	24.13262342135947
17	23.744769274511587	26.665699796711493	25.47771979289318	24.11181113588374
18	23.01273400506214	25.47911274113368	27.242093584817844	24.266059668986333
19	24.283430552926706	25.510413107479067	27.045401099445854	23.160755240148372
20	24.721676650828027	24.672759586146885	27.163760730822585	23.441803032202504
21	24.47393670934824	25.413644173830107	25.964882955475638	24.147536161346014
22	24.593771227097267	25.182947363763518	25.951854792520358	24.271426616618854
23	23.87632094510719	25.666464279481133	26.34298646464001	24.11422831077167
24	24.350496913800264	25.81206833967944	26.234623285342167	23.60281146117812
25	24.49741198410728	25.192206372656262	26.25244482900741	24.05793681422905
26	24.229802045667398	25.86557393974106	26.199348919604766	23.70527509498678
27	24.602620545331042	25.154965491755792	25.771386057243618	24.471027905669544
28	23.74427764572082	25.479235648331372	26.590603498922107	24.1858832070257
29	23.460443957185685	25.946323968624252	26.610145743355023	23.98308633083504
30	23.330080389501102	26.640585759316572	26.551273195660883	23.478060655521443
31	24.071251760645605	26.003311939287123	26.131913837138132	23.79352246292914
32	23.13338790412911	26.00589299043864	26.481461907372218	24.379257198060035
33	23.43832066160125	25.64360354071055	26.674590084011168	24.243485713677032
34	24.041180466277133	25.432530913208673	26.459297642721886	24.066990977792308
35	23.86837294632315	25.669004361566756	26.864891395103214	23.59773129700688
36	23.434059878747952	25.975534912608882	26.504445553340496	24.085959655302666
37	24.195060277786652	24.99489935129581	26.53943313561662	24.270607235300915
38	23.5903978342113	25.19990855704492	27.239020904875566	23.970672703868217
39	23.58208111383419	25.25001372463708	27.062362292727254	24.105542868801482
40	23.020559096648483	24.95794525385662	27.813530115950652	24.207965533544243
41	22.409710324122667	25.235961335034368	28.140831983402613	24.213496357440352
42	23.160550394818888	24.710737910233497	27.7817381208145	24.346973574133116
43	22.53573117082216	24.65256183665961	28.015466641757474	24.796240350760755
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	144.0
1	161.0
2	178.0
3	661.0
4	1144.0
5	1144.0
6	1659.5
7	2175.0
8	2221.0
9	2267.0
10	3313.5
11	4360.0
12	4360.0
13	7393.5
14	10427.0
15	14640.0
16	18853.0
17	18829.0
18	18805.0
19	18805.0
20	21691.0
21	24577.0
22	24232.0
23	23887.0
24	26848.0
25	29809.0
26	29809.0
27	34553.0
28	39297.0
29	49311.5
30	59326.0
31	66404.5
32	73483.0
33	73483.0
34	81368.5
35	89254.0
36	97044.5
37	104835.0
38	118301.0
39	131767.0
40	131767.0
41	139319.0
42	146871.0
43	144393.0
44	141915.0
45	149748.0
46	157581.0
47	157581.0
48	162690.5
49	167800.0
50	175862.0
51	183924.0
52	193172.5
53	202421.0
54	202421.0
55	185160.0
56	167899.0
57	158171.5
58	148444.0
59	134590.5
60	120737.0
61	120737.0
62	113315.0
63	105893.0
64	93181.5
65	80470.0
66	69784.5
67	59099.0
68	59099.0
69	50820.0
70	42541.0
71	37212.5
72	31884.0
73	25959.5
74	20035.0
75	20035.0
76	15911.5
77	11788.0
78	9839.0
79	7890.0
80	6025.0
81	4160.0
82	4160.0
83	3159.0
84	2158.0
85	1738.0
86	1318.0
87	1123.5
88	929.0
89	929.0
90	687.5
91	446.0
92	250.0
93	54.0
94	35.0
95	16.0
96	16.0
97	10.0
98	4.0
99	2.5
100	1.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	2440866.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	52.788696649523104
#Duplication Level	Percentage of deduplicated	Percentage of total
1	83.18987950112397	43.91485313295213
2	10.177790926160872	10.7454463552675
3	2.7431513928429965	4.344221602215171
4	1.1411643089264858	2.409623061247317
5	0.6297788710453457	1.662260288994594
6	0.40584435239358957	1.2854396643256414
7	0.2583917339504607	0.9548114002178606
8	0.1843935433314974	0.7787115858445689
9	0.14676907859570987	0.6972973530767048
>10	0.8880840060788002	9.034023487197059
>50	0.1045148718672471	3.8536135276062518
>100	0.1151584781038579	12.846924599002515
>500	0.011114885504326622	3.915896684062871
>1k	0.003730870658794873	2.7222699270470376
>5k	2.3317941617467957E-4	0.8346073309428277
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	7586	0.3107913338954289	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	6847	0.2805151941974693	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	5908	0.24204524132008884	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	3953	0.16195071749125106	No Hit
GCTTAGGACACAGAGCACATCAAAAGTTCCCAAAGAGGGCTTG	2500	0.10242266474275934	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	1.229071976913112E-4	0.0
2	0.0	4.096906589710373E-5	0.0	0.001229071976913112	0.0
3	0.0	4.096906589710373E-5	0.0	0.0019255460971638755	0.0
4	0.0	4.096906589710373E-5	0.0	0.003523339667150921	0.0
5	0.0	1.229071976913112E-4	0.0	0.0038920612602248547	0.0
6	0.0	1.229071976913112E-4	0.0	0.003973999392019062	0.0
7	0.0	1.229071976913112E-4	0.0	0.004957256973549552	0.0
8	0.0	1.229071976913112E-4	0.0	0.005203071368932174	0.0
9	4.096906589710373E-5	1.229071976913112E-4	0.0	0.00856253477249468	0.0
10	1.229071976913112E-4	1.229071976913112E-4	0.0	0.013151070152970299	0.0
11	1.229071976913112E-4	1.229071976913112E-4	0.0	0.014625956525266033	0.0
12	1.229071976913112E-4	1.229071976913112E-4	0.0	0.01622375009525308	0.0
13	1.229071976913112E-4	1.229071976913112E-4	0.0	0.017288945808577774	0.0
14	1.229071976913112E-4	1.229071976913112E-4	0.0	0.018313172456005368	0.0
15	1.229071976913112E-4	1.229071976913112E-4	0.0	0.02007484228958083	0.0
16	1.229071976913112E-4	1.229071976913112E-4	0.0	0.022901707836480985	0.0
17	1.229071976913112E-4	1.229071976913112E-4	0.0	0.02593341871286666	0.0
18	1.6387626358841493E-4	1.229071976913112E-4	0.0	0.027613150414647916	0.0
19	2.0484532948551867E-4	1.229071976913112E-4	0.0	0.029702572775400206	0.0
20	2.0484532948551867E-4	1.229071976913112E-4	0.0	0.03244750019050616	0.0
21	2.0484532948551867E-4	1.229071976913112E-4	0.0	0.0361756851871426	0.0
22	2.0484532948551867E-4	1.6387626358841493E-4	0.0	0.04314042638965023	0.0
23	2.0484532948551867E-4	1.6387626358841493E-4	0.0	0.052522342480086984	0.0
24	2.0484532948551867E-4	1.6387626358841493E-4	0.0	0.06624697955561673	0.0
25	2.0484532948551867E-4	1.6387626358841493E-4	0.0	0.07058970054070973	0.0
26	2.0484532948551867E-4	1.6387626358841493E-4	0.0	0.07857866839064497	0.0
27	2.0484532948551867E-4	1.6387626358841493E-4	0.0	0.08857512046953828	0.0
28	2.0484532948551867E-4	1.6387626358841493E-4	0.0	0.11057550885628298	0.0
29	2.0484532948551867E-4	2.0484532948551867E-4	0.0	0.1448666170121588	0.0
30	2.0484532948551867E-4	2.0484532948551867E-4	0.0	0.19579116592225873	0.0
31	2.0484532948551867E-4	2.0484532948551867E-4	0.0	0.38449468344431853	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CATACCG	75	2.0684666E-4	17.266666	5
GACCGAT	65	0.0015806217	17.076923	7
ACCGTCC	90	4.4492495E-5	16.444445	8
GGTATCA	4050	0.0	15.302469	1
TAATACT	385	0.0	14.896104	4
TAGGACA	1045	0.0	14.69378	4
TAGCACT	425	0.0	14.364706	4
GCTTAGG	990	0.0	14.202021	1
GACGGAC	275	0.0	14.127274	7
TCTAGCG	225	2.7284841E-11	13.9777775	28
TTAGGAC	1100	0.0	13.959091	3
GTAGAAC	785	0.0	13.904459	3
CGGCATA	80	0.0063012964	13.875	36
CTAATAC	455	0.0	13.824175	3
GTCGTTG	295	0.0	13.79661	1
ATCTCGC	565	0.0	13.752213	11
TAGAACA	825	0.0	13.678788	4
CTTAGGA	1080	0.0	13.532408	2
CGAGCGT	110	2.458133E-4	13.454545	6
TAGACTG	210	2.044544E-9	13.214285	5
>>END_MODULE