FastQCFastQC Report
Fri 10 Feb 2017
ERR1630594.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1630594.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1201307
Sequences flagged as poor quality0
Sequence length43
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT231141.9240710326336234No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT211731.7624970136692786No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT168261.4006411350304293No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT120791.0054881891140233No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT33920.28235913051368217No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT27160.2260870868146111No Hit
AACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT24580.2046104784205869No Hit
CTTATACACATCTCCGAGCCCACGAGACGCTCATGAATCTCGT23930.19919970498798392No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT23740.19761809429229998No Hit
GTGTAGCGCGCGTGCAGCCCCGGACATCTAAGGGCATCACAGA22420.1866300620907062No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT18430.15341623748134325No Hit
GGGTAGGCACACGCTGAGCCAGTCAGTGTAGCGCGCGTGCAGC17730.1475892507077708No Hit
CTTCTTAGAGGGACAAGTGGCGTTCAGCCACCCGAGATTGAGC17410.14492548532556623No Hit
TCTCCGAGCCCACGAGACGCTCATGAATCTCGTATGCCGTCTT16900.14068010924767774TruSeq Adapter, Index 8 (95% over 22bp)
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTT16050.1336044824511969No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTG14790.1231159062587665No Hit
TGCCTACCCTACGCCGGCAGGCGCGGGTAACCCGTTGAACCCC14740.12269969291779705No Hit
GTACATGGGGAATAATTGCAATCCCCGATCCCCATCACGAATG14510.1207851115493375No Hit
GGATTACTCCGGTCTGAACTCAGATCACGTAGGACTTTAATCG14070.11712243414880627No Hit
TCTTAGAGGGACAAGTGGCGTTCAGCCACCCGAGATTGAGCAA14040.11687270614422458No Hit
TCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTT12140.10105659918738508No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGAATAT358.86963E-426.4285725
GCCGTCT2750.026.23636236
CCGTCTT2800.025.76785737
TTGTACT400.001931237923.1254
CGTTACA400.001931237923.12529
TGACGCA502.7022016E-422.19999928
GTATCGA502.7022016E-422.19999911
CTACCCG502.7022016E-422.19999910
CAGTCGG2800.021.8035710
CCTTGCT855.1812094E-821.76470615
GCAGTCG2950.021.3220339
AATACCC806.960654E-720.81255
GCTCTAG450.003825907820.5555551
GTCTTAC450.003825907820.5555551
GTAGCAG555.143279E-420.18181828
GCACTAT555.143279E-420.18181817
AGCACTA555.143279E-420.18181816
TATTAGA2950.020.0677972
GCTATAT851.2454038E-619.58823619
TCGGTGA3100.019.09677313
Produced by FastQC (version 0.11.5)