FastQCFastQC Report
Fri 10 Feb 2017
ERR1630593.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1630593.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2639731
Sequences flagged as poor quality0
Sequence length43
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT119450.4525082290581881No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT114790.43485491514097463No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT98310.37242431141657995No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT69150.2619585101663768No Hit
ACCTAGGCCTGGTCAGCATGTTGATGTATCTACGGAGATCAGC53730.20354346711842988No Hit
GCTTTATTGAGCCTGTGTGGGAGCAGGGAGCAAGCTTTGGCCA51580.19539869782186137No Hit
GTGTGGGAGCAGGGAGCAAGCTTTGGCCAGAGCCAAGGGTGCA47460.17979104689076272No Hit
GGCCTAGGTATGGGAAAAGACACAAAGAGGACACGCTGGCCTT47070.1783136236230131No Hit
GTATGCAGCTGATCTCCGTAGATACATCAACATGCTGACCAGG35580.13478646119623552No Hit
GGGTACACTGGCTCCAGTGGGGCTCCCTGGGCACCCAGCAGTG30240.1145571272224329No Hit
AGTGTACCCAGGGGACAATGCCACACCAGAGCAGATGGCCCAG30160.11425406603930476No Hit
GCTCATGGAGTCGTAGGAGACAGAAGGTGGCATTATAAGTCCA28940.1096323829966008No Hit
GCTGCATACTGGGCCATCTGCTCTGGTGTGGCATTGTCCCCTG28290.10717001088368473No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGACGG3600.017.986115
GCTTTAT15100.017.8874191
CGAACGA2850.016.87719316
GGTATCA62050.015.8614021
TCTAGCG2950.015.67796628
GACGGAC3950.015.4556967
GTATAGA2700.015.0740751
ACCGATC2251.8189894E-1214.827
GTATACG1001.0942173E-414.7999991
TAACGAA3300.014.57575713
CGGACCA4200.014.5357149
TATTGAG17500.014.3771435
ACGAACG3350.014.3582115
TTTCGGA4300.014.19767430
TATCTCG2353.6379788E-1214.17021436
TTTATTG19650.014.1221383
ATAACGA3550.014.07042312
GTAAACG2900.014.03448327
TACCGTG800.006301476613.8757
ATCTCGT2405.456968E-1213.87499937
Produced by FastQC (version 0.11.5)