##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1630593.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	2639731
Sequences flagged as poor quality	0
Sequence length	43
%GC	49
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.121018012820244	33.0	31.0	34.0	30.0	34.0
2	32.27612548399818	34.0	31.0	34.0	30.0	34.0
3	32.38347771041822	34.0	31.0	34.0	30.0	34.0
4	35.94988656041089	37.0	35.0	37.0	35.0	37.0
5	35.908717971641806	37.0	35.0	37.0	35.0	37.0
6	35.988997363746535	37.0	35.0	37.0	35.0	37.0
7	35.93771410799055	37.0	35.0	37.0	35.0	37.0
8	35.94141183325119	37.0	35.0	37.0	35.0	37.0
9	37.648406220179254	39.0	37.0	39.0	35.0	39.0
10	37.57388536938044	39.0	37.0	39.0	35.0	39.0
11	37.66629667947227	39.0	37.0	39.0	35.0	39.0
12	37.61753981750413	39.0	37.0	39.0	35.0	39.0
13	37.65702679553333	39.0	37.0	39.0	35.0	39.0
14	38.95945609609464	40.0	38.0	41.0	36.0	41.0
15	38.972165345635595	40.0	38.0	41.0	36.0	41.0
16	38.92923521373958	40.0	38.0	41.0	36.0	41.0
17	38.93609235183433	40.0	38.0	41.0	36.0	41.0
18	38.92379071958469	40.0	38.0	41.0	36.0	41.0
19	38.97712797250932	40.0	38.0	41.0	35.0	41.0
20	38.95408547310313	40.0	38.0	41.0	35.0	41.0
21	38.90587450009111	40.0	38.0	41.0	35.0	41.0
22	38.843950008542535	40.0	38.0	41.0	35.0	41.0
23	38.79980535895513	40.0	38.0	41.0	35.0	41.0
24	38.74337347252428	40.0	38.0	41.0	35.0	41.0
25	38.69057150141435	40.0	38.0	41.0	35.0	41.0
26	38.55808034985383	40.0	38.0	41.0	34.0	41.0
27	38.4303351364211	40.0	38.0	41.0	34.0	41.0
28	38.331272769839046	40.0	38.0	41.0	34.0	41.0
29	38.266467302918365	40.0	38.0	41.0	34.0	41.0
30	38.182625426605966	40.0	38.0	41.0	34.0	41.0
31	38.1298617169704	40.0	38.0	41.0	34.0	41.0
32	38.038957378611684	40.0	37.0	41.0	33.0	41.0
33	37.943645394170844	40.0	37.0	41.0	33.0	41.0
34	37.88572812911619	40.0	37.0	41.0	33.0	41.0
35	37.81167058310108	40.0	37.0	41.0	33.0	41.0
36	37.76776194241004	40.0	37.0	41.0	33.0	41.0
37	37.62569557276859	40.0	37.0	41.0	33.0	41.0
38	37.47032974193204	40.0	37.0	41.0	32.0	41.0
39	37.39602785283804	40.0	36.0	41.0	32.0	41.0
40	37.197892512532526	40.0	36.0	41.0	31.0	41.0
41	37.137590913619604	40.0	36.0	41.0	31.0	41.0
42	36.98970425395618	40.0	36.0	41.0	31.0	41.0
43	36.205777028038085	39.0	35.0	40.0	30.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	2.0
8	3.0
9	7.0
10	9.0
11	9.0
12	8.0
13	6.0
14	5.0
15	5.0
16	13.0
17	29.0
18	54.0
19	132.0
20	261.0
21	563.0
22	991.0
23	1774.0
24	3125.0
25	5052.0
26	7830.0
27	12084.0
28	17975.0
29	25708.0
30	34652.0
31	45314.0
32	59320.0
33	77532.0
34	103793.0
35	139596.0
36	198904.0
37	316526.0
38	587585.0
39	1000864.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	45.362766130336766	18.583787514712675	11.619365761132478	24.434080593818084
2	19.1521029983737	20.635019250067526	33.67013532818306	26.542742423375714
3	21.45798189285196	23.314117991568082	28.24249895159772	26.985401163982235
4	14.450146624788662	14.586258978661082	34.15961702158288	36.803977374967374
5	14.431546244674173	37.245764814672405	33.96751411412754	14.355174826525886
6	35.941427365136825	33.74771899106386	15.303604799125365	15.007248844673946
7	29.715111123065192	29.896720537054723	21.302246327371993	19.085922012508092
8	29.002652164178848	31.245494332566466	19.853613872019533	19.89823963123515
9	27.285848444405886	13.348254045582674	19.84338555708896	39.52251195292247
10	17.817610961116873	26.671808604740406	32.10361207259376	23.40696836154896
11	35.60790853310432	21.809760161167937	21.14207849208878	21.440252813638967
12	23.567628671254763	25.420734158139595	28.018423089322358	22.993214081283284
13	31.067180708943447	19.465657674967638	24.983757814716725	24.483403801372187
14	23.335067095851812	20.61729017085453	25.54533018705315	30.502312546240507
15	26.290936462844133	27.55958845806637	22.513809172222473	23.635665906867025
16	25.208212503471	25.933059088217703	24.3285774194416	24.5301509888697
17	24.207277180894568	25.887069553678003	25.288296421112605	24.61735684431482
18	23.44636631535562	24.464007885651984	27.638763192158596	24.450862606833805
19	24.96792286789828	24.57417062571906	27.477761938621775	22.98014456776088
20	25.655682340359682	23.98441356335172	26.424586444603637	23.935317651684965
21	25.410543725856915	24.274670411492686	26.563653645011552	23.751132217638844
22	25.340195648723295	24.51386145027656	26.74681624756462	23.39912665343552
23	24.630918832259802	24.454612988975015	26.234718613373865	24.679749565391322
24	24.014416620481406	25.1871118685957	26.991954862067384	23.806516648855506
25	24.42923161488803	25.263748465279228	26.149823599450094	24.157196320382646
26	24.38221924885528	25.409293598476513	26.486789752440686	23.721697400227523
27	24.621789114118066	24.285580614085298	26.57827634709749	24.514353924699144
28	23.830572130266304	24.99466801730934	26.738936656803286	24.43582319562107
29	24.05332209986548	25.586735921198034	27.00241047288531	23.357531506051185
30	24.241068502813352	25.39391324343276	26.18622882407336	24.178789429680524
31	23.38336747191286	26.2484700145583	26.693363831390393	23.674798682138444
32	23.233465834208104	25.407778292560874	26.491941792553863	24.86681408067716
33	22.72352751094714	25.96590334393921	26.971346701614674	24.33922244349898
34	24.76953144089303	24.49442765190847	26.58585287667569	24.150188030522806
35	23.611080068385757	24.95894468034811	26.79970042402048	24.630274827245653
36	22.93051829902365	26.05981442806104	26.971498232206237	24.03816904070907
37	23.781627749191113	24.74369547503136	27.29702382553374	24.177652950243793
38	23.888721994779015	25.03895283269394	26.751475813255215	24.320849359271833
39	23.618202006189268	24.71176040285923	27.23591911448553	24.434118476465976
40	22.759856970274623	24.575041926620553	27.764912409635677	24.900188693469143
41	21.798130188265393	25.02160257995985	28.294852770983102	24.88541446079165
42	22.56328391036814	24.754870856159208	28.643335248932562	24.03850998454009
43	22.871307720369995	24.500261579683688	27.976524880754894	24.651905819191423
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	424.0
1	458.0
2	492.0
3	1417.0
4	2342.0
5	2342.0
6	3243.0
7	4144.0
8	3963.5
9	3783.0
10	5474.0
11	7165.0
12	7165.0
13	12169.0
14	17173.0
15	23139.5
16	29106.0
17	27998.0
18	26890.0
19	26890.0
20	30121.0
21	33352.0
22	30678.5
23	28005.0
24	31958.5
25	35912.0
26	35912.0
27	41375.5
28	46839.0
29	53353.0
30	59867.0
31	67095.0
32	74323.0
33	74323.0
34	82665.0
35	91007.0
36	99539.5
37	108072.0
38	116539.0
39	125006.0
40	125006.0
41	130474.0
42	135942.0
43	138122.5
44	140303.0
45	145709.0
46	151115.0
47	151115.0
48	161307.5
49	171500.0
50	171614.0
51	171728.0
52	172068.0
53	172408.0
54	172408.0
55	168562.5
56	164717.0
57	161645.5
58	158574.0
59	162203.0
60	165832.0
61	165832.0
62	153087.0
63	140342.0
64	130596.0
65	120850.0
66	104880.5
67	88911.0
68	88911.0
69	78491.0
70	68071.0
71	57823.0
72	47575.0
73	35094.5
74	22614.0
75	22614.0
76	16238.0
77	9862.0
78	8001.0
79	6140.0
80	4974.0
81	3808.0
82	3808.0
83	2995.5
84	2183.0
85	1823.5
86	1464.0
87	1328.5
88	1193.0
89	1193.0
90	890.5
91	588.0
92	331.5
93	75.0
94	48.0
95	21.0
96	21.0
97	15.5
98	10.0
99	6.5
100	3.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	2639731.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	53.9146625643103
#Duplication Level	Percentage of deduplicated	Percentage of total
1	84.60932420435144	45.616831642719404
2	9.920280362864533	10.6969713661439
3	2.4402211077552156	3.946910928207898
4	0.8910708658360189	1.921671402097471
5	0.46069300140332986	1.241905385819993
6	0.2790970123532946	0.9028452746241012
7	0.20338091915011206	0.7675649539598295
8	0.1383980058842796	0.5969345429459493
9	0.11158934569199506	0.5414671726880479
>10	0.7459528981864327	7.9956284264070225
>50	0.09172421091523636	3.4754801357122562
>100	0.08943368141158219	10.243620594936898
>500	0.012274093071886637	4.635165521738777
>1k	0.006137046535942713	5.4887515843750325
>5k	2.8216305912380296E-4	1.0373938259908382
>10k+	1.4108152956190148E-4	0.8908572416324886
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	11945	0.4525082290581881	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	11479	0.43485491514097463	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	9831	0.37242431141657995	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	6915	0.2619585101663768	No Hit
ACCTAGGCCTGGTCAGCATGTTGATGTATCTACGGAGATCAGC	5373	0.20354346711842988	No Hit
GCTTTATTGAGCCTGTGTGGGAGCAGGGAGCAAGCTTTGGCCA	5158	0.19539869782186137	No Hit
GTGTGGGAGCAGGGAGCAAGCTTTGGCCAGAGCCAAGGGTGCA	4746	0.17979104689076272	No Hit
GGCCTAGGTATGGGAAAAGACACAAAGAGGACACGCTGGCCTT	4707	0.1783136236230131	No Hit
GTATGCAGCTGATCTCCGTAGATACATCAACATGCTGACCAGG	3558	0.13478646119623552	No Hit
GGGTACACTGGCTCCAGTGGGGCTCCCTGGGCACCCAGCAGTG	3024	0.1145571272224329	No Hit
AGTGTACCCAGGGGACAATGCCACACCAGAGCAGATGGCCCAG	3016	0.11425406603930476	No Hit
GCTCATGGAGTCGTAGGAGACAGAAGGTGGCATTATAAGTCCA	2894	0.1096323829966008	No Hit
GCTGCATACTGGGCCATCTGCTCTGGTGTGGCATTGTCCCCTG	2829	0.10717001088368473	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	1.5153059156406467E-4	0.0
2	0.0	0.0	0.0	0.0010228314930574365	0.0
3	0.0	0.0	0.0	0.0021214282818969054	0.0
4	0.0	0.0	0.0	0.00356096890175552	0.0
5	0.0	0.0	0.0	0.004015560676447713	0.0
6	0.0	0.0	0.0	0.0041292086201207626	0.0
7	0.0	0.0	0.0	0.004697448338486005	0.0
8	0.0	0.0	0.0	0.00503839216950515	0.0
9	0.0	0.0	0.0	0.008220534592350509	0.0
10	3.788264789101617E-5	0.0	0.0	0.011819386141997044	0.0
11	3.788264789101617E-5	0.0	0.0	0.013334692057637692	0.0
12	3.788264789101617E-5	0.0	0.0	0.014357523550695127	0.0
13	3.788264789101617E-5	0.0	0.0	0.015190941804297484	0.0
14	3.788264789101617E-5	0.0	0.0	0.016478951832592032	0.0
15	3.788264789101617E-5	0.0	0.0	0.01860038011448894	0.0
16	3.788264789101617E-5	0.0	0.0	0.02246441019937259	0.0
17	7.576529578203233E-5	0.0	0.0	0.027427037073095704	0.0
18	1.5153059156406467E-4	0.0	0.0	0.029889409186011757	0.0
19	1.5153059156406467E-4	0.0	0.0	0.03360190867933134	0.0
20	1.5153059156406467E-4	0.0	0.0	0.0370113469895228	0.0
21	1.5153059156406467E-4	0.0	0.0	0.04269374417317522	0.0
22	1.5153059156406467E-4	0.0	0.0	0.05314935499109568	0.0
23	1.5153059156406467E-4	0.0	0.0	0.06902218445743145	0.0
24	1.5153059156406467E-4	0.0	3.788264789101617E-5	0.09319131381189977	0.0
25	1.8941323945508083E-4	0.0	3.788264789101617E-5	0.10035113426330183	0.0
26	1.8941323945508083E-4	0.0	3.788264789101617E-5	0.11080674508122229	0.0
27	1.8941323945508083E-4	0.0	3.788264789101617E-5	0.1260734521813018	0.0
28	2.2729588734609702E-4	0.0	3.788264789101617E-5	0.15596286136731358	0.0
29	2.651785352371132E-4	0.0	3.788264789101617E-5	0.2059679565834549	0.0
30	2.651785352371132E-4	0.0	3.788264789101617E-5	0.27351271777313674	0.0
31	2.651785352371132E-4	0.0	3.788264789101617E-5	0.5155828377967301	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
AAGACGG	360	0.0	17.98611	5
GCTTTAT	1510	0.0	17.887419	1
CGAACGA	285	0.0	16.877193	16
GGTATCA	6205	0.0	15.861402	1
TCTAGCG	295	0.0	15.677966	28
GACGGAC	395	0.0	15.455696	7
GTATAGA	270	0.0	15.074075	1
ACCGATC	225	1.8189894E-12	14.8	27
GTATACG	100	1.0942173E-4	14.799999	1
TAACGAA	330	0.0	14.575757	13
CGGACCA	420	0.0	14.535714	9
TATTGAG	1750	0.0	14.377143	5
ACGAACG	335	0.0	14.35821	15
TTTCGGA	430	0.0	14.197674	30
TATCTCG	235	3.6379788E-12	14.170214	36
TTTATTG	1965	0.0	14.122138	3
ATAACGA	355	0.0	14.070423	12
GTAAACG	290	0.0	14.034483	27
TACCGTG	80	0.0063014766	13.875	7
ATCTCGT	240	5.456968E-12	13.874999	37
>>END_MODULE