FastQCFastQC Report
Fri 10 Feb 2017
ERR1630592.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1630592.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1899267
Sequences flagged as poor quality0
Sequence length43
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT29090.1531643523527761No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT28690.15105827669306104No Hit
GCTTTATTGAGCCTGTGTGGGAGCAGGGAGCAAGCTTTGGCCA24880.13099790603427533No Hit
GTGTGGGAGCAGGGAGCAAGCTTTGGCCAGAGCCAAGGGTGCA23670.12462702716363734No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT23150.12188912880600779No Hit
ACCTAGGCCTGGTCAGCATGTTGATGTATCTACGGAGATCAGC20480.10783107377740993No Hit
GGCCTAGGTATGGGAAAAGACACAAAGAGGACACGCTGGCCTT19640.10340831489200834No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA14800.024.01
GCTTTAT9250.016.41
GTACTAA1302.5913687E-715.6538461
TCTAGCG1902.7284841E-1115.57894828
CTAGCGG1954.1836756E-1115.17948729
ATACCGC2450.015.1020427
GTATCAA23600.015.0508472
GACGGAC2350.014.9574467
CGAGCCG2650.014.660377515
TATTGAG9500.014.6052635
GCGCAAG3050.014.5573771
ACGGACC2550.014.5098058
ATAATAC2301.8189894E-1214.4782613
CGAACGA908.2775194E-414.38888816
AAGTACG1702.4392648E-814.14705935
AAGACGG2900.014.0344835
CTTATAC9850.013.89847837
CGCAATA2009.840733E-1013.87500136
CGAAAGC2551.8189894E-1213.78431519
GTACTGC3100.013.7258076
Produced by FastQC (version 0.11.5)