FastQCFastQC Report
Fri 10 Feb 2017
ERR1630591.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1630591.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2323877
Sequences flagged as poor quality0
Sequence length43
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT73390.31580845285701437No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT68150.2932599272680955No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT58320.2509599260201809No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT38790.1669193335103364No Hit
GCTTAGGACACAGAGCACATCAAAAGTTCCCAAAGAGGGCTTG37660.16205676978600847No Hit
GTTCTACAGCACACTACCAGAAGACAGCAGAAATGAAAAGCAT36590.15745239528598115No Hit
TCCCAGAAGAGGTCGCCATTGTTGAAGAACTTGGCCGCAGACA32610.14032584340737483No Hit
CTGTAGAACAGAGCAGGTGAAGAGAGAGCAAGCCCTCTTTGGG32380.13933611804755588No Hit
CTTCTGGGAAATCTCGCCTTCCTCGGCCTTTCACCAGCCAAGC31890.13722757271576766No Hit
GAATACACCTCTTAAATTTACAGGACTTAACATTTCAAACATC26480.11394751099133044No Hit
ATTGAAAGCTGAGTATTTTTAAGACAAAGGTTTCAGGAAGAAA24080.10361994201930652No Hit
CTCCAGGCGTGCCCAAGATTTTGTGCAGTGGTTGATGAATACC23250.1000483244164816No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGACGG3650.018.7534245
TATACTG1800.017.4722215
CGCAAGA3850.017.2987022
CGGACCA3700.017.0000029
ACGAACG2100.016.73809415
GACGGAC3650.016.7260277
GCTACGA1005.88299E-616.6500022
GGTATCA32850.016.3881261
CGAACGA2050.016.24390416
GCGTTAT3000.015.4166671
TCTAGCG2300.015.28260928
TCGCCAT8600.015.27325513
CGAACTA1353.9769293E-715.07407524
ATTGCCG3100.014.91935511
ATCTCGC9600.014.83854211
TAACGAA2251.8189894E-1214.813
CTAGTAC750.004106083414.83
ACGGACC4250.014.88
CTTATAC16450.014.73252237
CTAGCGG2400.014.64583429
Produced by FastQC (version 0.11.5)