##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1630591.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	2323877
Sequences flagged as poor quality	0
Sequence length	43
%GC	47
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.14188530632215	33.0	31.0	34.0	30.0	34.0
2	32.31868726270797	34.0	31.0	34.0	30.0	34.0
3	32.416045255407234	34.0	31.0	34.0	30.0	34.0
4	35.98354387947383	37.0	35.0	37.0	35.0	37.0
5	35.94122795655708	37.0	35.0	37.0	35.0	37.0
6	36.01854444103539	37.0	35.0	37.0	35.0	37.0
7	35.967658787448734	37.0	35.0	37.0	35.0	37.0
8	35.96507388299811	37.0	35.0	37.0	35.0	37.0
9	37.69221090444976	39.0	37.0	39.0	35.0	39.0
10	37.61657092866791	39.0	37.0	39.0	35.0	39.0
11	37.70475373696628	39.0	37.0	39.0	35.0	39.0
12	37.66168174993771	39.0	37.0	39.0	35.0	39.0
13	37.690162172954935	39.0	37.0	39.0	35.0	39.0
14	39.02152179310695	40.0	38.0	41.0	36.0	41.0
15	39.0394616410421	40.0	38.0	41.0	36.0	41.0
16	39.00341369186063	40.0	38.0	41.0	36.0	41.0
17	38.98956442186914	40.0	38.0	41.0	36.0	41.0
18	38.96589320346989	40.0	38.0	41.0	36.0	41.0
19	39.036884482268206	40.0	39.0	41.0	36.0	41.0
20	39.00441933888928	40.0	39.0	41.0	35.0	41.0
21	38.96411729192208	40.0	38.0	41.0	35.0	41.0
22	38.91009377862942	40.0	38.0	41.0	35.0	41.0
23	38.86267646695587	40.0	38.0	41.0	35.0	41.0
24	38.82058688992576	40.0	38.0	41.0	35.0	41.0
25	38.7723687613415	40.0	38.0	41.0	35.0	41.0
26	38.655730918632955	40.0	38.0	41.0	34.0	41.0
27	38.53985129161311	40.0	38.0	41.0	34.0	41.0
28	38.454112244322744	40.0	38.0	41.0	34.0	41.0
29	38.39198503191004	40.0	38.0	41.0	34.0	41.0
30	38.321833728721444	40.0	38.0	41.0	34.0	41.0
31	38.28439672151323	40.0	38.0	41.0	34.0	41.0
32	38.18743677053476	40.0	38.0	41.0	34.0	41.0
33	38.1269352896044	40.0	38.0	41.0	33.0	41.0
34	38.07408352507469	40.0	38.0	41.0	33.0	41.0
35	38.02594672609609	40.0	37.0	41.0	33.0	41.0
36	37.98907041981998	40.0	37.0	41.0	33.0	41.0
37	37.874082836570096	40.0	37.0	41.0	33.0	41.0
38	37.72904934297297	40.0	37.0	41.0	33.0	41.0
39	37.68451428367336	40.0	37.0	41.0	33.0	41.0
40	37.53457261292228	40.0	37.0	41.0	33.0	41.0
41	37.48956162481922	40.0	37.0	41.0	32.0	41.0
42	37.377201116926585	40.0	37.0	41.0	32.0	41.0
43	36.60664785614729	39.0	35.0	40.0	30.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	1.0
8	3.0
9	3.0
10	7.0
11	2.0
12	2.0
13	3.0
14	3.0
15	3.0
16	4.0
17	29.0
18	65.0
19	104.0
20	191.0
21	428.0
22	783.0
23	1451.0
24	2319.0
25	3898.0
26	6034.0
27	9239.0
28	14088.0
29	19846.0
30	27489.0
31	37002.0
32	48921.0
33	64307.0
34	86927.0
35	119328.0
36	169778.0
37	266171.0
38	506253.0
39	939195.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	43.337878898065604	19.401801386217947	12.368124474746296	24.892195240970157
2	18.171701858575133	21.46335627918345	35.7544740965206	24.61046776572082
3	20.245864991993983	23.687269162696648	30.232107809492497	25.83475803581687
4	14.701939904736783	15.613089677293592	34.35728310921791	35.327687308751706
5	13.891053614283372	37.66649439707868	34.542792066877894	13.899659921760058
6	34.473382197078415	34.61301953588766	16.002869342912724	14.9107289241212
7	28.082854643339555	31.06029277797405	21.835449982937995	19.0214025957484
8	26.14566089341217	34.370493791194626	20.542997757626587	18.94084755776661
9	26.081328745023942	14.42950724156227	20.29875075143822	39.19041326197557
10	16.587667935953583	27.632185352322864	33.429437100156335	22.350709611567222
11	34.47015483177466	22.218344602575783	22.38866342753941	20.92283713811015
12	20.948354839778524	26.106975541304468	29.254087027841834	23.690582591075174
13	28.9437866117699	20.450350857640057	26.30883648317015	24.29702604741989
14	22.36499608197852	21.793322107839614	25.660695467100886	30.180986343080978
15	25.153870019798813	28.395521793967582	23.30321269154951	23.1473954946841
16	24.712065225483105	27.379504164807344	24.48300835199109	23.42542225771846
17	23.280449008273674	27.248473133474793	26.00400107234591	23.467076785905622
18	22.325277972973613	25.912171771569664	27.845492683132537	23.91705757232418
19	23.68025502210315	26.291795994366314	27.751942120860956	22.276006862669583
20	23.891453807581037	25.379140118001082	27.96628220856784	22.763123865850044
21	23.587823279803537	26.51551695722278	26.577267213368007	23.31939254960568
22	23.741962246710994	25.94156231160255	26.656875557527353	23.659599884159103
23	22.949450422720307	26.51840007022747	27.047731011581078	23.484418495471147
24	24.35434405521463	26.35083526365638	26.637253176480513	22.657567504648483
25	23.842182697277007	25.660566372488734	27.1365050732031	23.36074585703116
26	23.730386763154847	26.263524274305393	26.823794891037693	23.182294071502064
27	23.836889818178843	25.919142880625785	26.43732865379708	23.806638647398294
28	22.871089993145077	26.308363136258933	27.332126442148187	23.488420428447803
29	22.946782467402535	26.914118088005516	27.107157564707602	23.03194187988435
30	22.527612261750516	27.54650956139245	27.03284209964641	22.893036077210628
31	23.384671391816347	26.598051445924202	26.934385942113114	23.082891220146333
32	22.49142273881105	26.548350020246335	27.149199376731215	23.8110278642114
33	22.76295173970051	26.27647676705781	27.33346041980707	23.62711107343461
34	23.21874178366583	26.30582427555331	26.936623582057056	23.538810358723804
35	23.356485734830198	26.254960998366094	27.15660080116116	23.231952465642543
36	22.881116341355415	26.464610648498176	27.159182693404173	23.495090316742235
37	23.691271095673308	25.05382169538233	27.276228475087105	23.978678733857254
38	22.75477574759766	25.63169221090445	28.26216705961632	23.35136498188157
39	22.75421633761167	25.542659960058128	28.18277387314389	23.520349829186312
40	22.50786078609152	24.942068792797553	28.927434627564196	23.62263579354673
41	21.676964830754812	25.735441247535906	29.07378488620525	23.51380903550403
42	22.617376048732353	24.86009371408211	28.677722616128133	23.844807621057395
43	21.60927622245067	25.120993925237865	28.880745409503174	24.38898444280829
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	272.0
1	298.5
2	325.0
3	836.5
4	1348.0
5	1348.0
6	1901.0
7	2454.0
8	2448.5
9	2443.0
10	3693.5
11	4944.0
12	4944.0
13	8061.0
14	11178.0
15	15990.0
16	20802.0
17	21033.0
18	21264.0
19	21264.0
20	24839.5
21	28415.0
22	30096.5
23	31778.0
24	34813.5
25	37849.0
26	37849.0
27	41484.0
28	45119.0
29	57528.5
30	69938.0
31	73238.0
32	76538.0
33	76538.0
34	83502.5
35	90467.0
36	96272.0
37	102077.0
38	118230.0
39	134383.0
40	134383.0
41	142884.5
42	151386.0
43	143279.5
44	135173.0
45	142406.5
46	149640.0
47	149640.0
48	156096.5
49	162553.0
50	167810.5
51	173068.0
52	180422.5
53	187777.0
54	187777.0
55	164477.5
56	141178.0
57	132579.5
58	123981.0
59	112343.0
60	100705.0
61	100705.0
62	93918.5
63	87132.0
64	78094.5
65	69057.0
66	60464.5
67	51872.0
68	51872.0
69	44961.0
70	38050.0
71	32911.5
72	27773.0
73	22563.0
74	17353.0
75	17353.0
76	13693.5
77	10034.0
78	8393.0
79	6752.0
80	5346.0
81	3940.0
82	3940.0
83	2989.0
84	2038.0
85	1675.0
86	1312.0
87	1145.5
88	979.0
89	979.0
90	721.0
91	463.0
92	254.0
93	45.0
94	30.5
95	16.0
96	16.0
97	9.5
98	3.0
99	3.0
100	3.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	2323877.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	48.59597229839347
#Duplication Level	Percentage of deduplicated	Percentage of total
1	84.41359279074045	41.0216061686669
2	9.321006934400401	9.059267895545107
3	2.4993185690681177	3.6437044784188397
4	1.0619528753758936	2.0642653005586484
5	0.5735314996572667	1.3935660434800299
6	0.35342465407771867	1.0305008819478065
7	0.24132739857097846	0.820927770305904
8	0.18925309640671992	0.735755058429292
9	0.1381575074091964	0.6042508562585158
>10	0.9212464311685551	8.75569234168999
>50	0.12472808733039656	4.270124541363478
>100	0.13662907944516792	14.255913868946262
>500	0.01908481585948464	6.201761232089327
>1k	0.006479960733685484	5.280527775137553
>5k	2.662997561788555E-4	0.8621357871623041
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	7339	0.31580845285701437	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	6815	0.2932599272680955	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	5832	0.2509599260201809	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	3879	0.1669193335103364	No Hit
GCTTAGGACACAGAGCACATCAAAAGTTCCCAAAGAGGGCTTG	3766	0.16205676978600847	No Hit
GTTCTACAGCACACTACCAGAAGACAGCAGAAATGAAAAGCAT	3659	0.15745239528598115	No Hit
TCCCAGAAGAGGTCGCCATTGTTGAAGAACTTGGCCGCAGACA	3261	0.14032584340737483	No Hit
CTGTAGAACAGAGCAGGTGAAGAGAGAGCAAGCCCTCTTTGGG	3238	0.13933611804755588	No Hit
CTTCTGGGAAATCTCGCCTTCCTCGGCCTTTCACCAGCCAAGC	3189	0.13722757271576766	No Hit
GAATACACCTCTTAAATTTACAGGACTTAACATTTCAAACATC	2648	0.11394751099133044	No Hit
ATTGAAAGCTGAGTATTTTTAAGACAAAGGTTTCAGGAAGAAA	2408	0.10361994201930652	No Hit
CTCCAGGCGTGCCCAAGATTTTGTGCAGTGGTTGATGAATACC	2325	0.1000483244164816	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	1.2909461215029883E-4	0.0
2	0.0	4.303153738343295E-5	0.0	0.001549135345803586	0.0
3	0.0	4.303153738343295E-5	0.0	0.0026679553177728424	0.0
4	0.0	4.303153738343295E-5	0.0	0.0042601222009598615	0.0
5	0.0	4.303153738343295E-5	0.0	0.004862563724327923	0.0
6	0.0	4.303153738343295E-5	0.0	0.005077721411245088	0.0
7	0.0	4.303153738343295E-5	0.0	0.005938352158913746	0.0
8	0.0	4.303153738343295E-5	0.0	0.006540793682281808	0.0
9	0.0	4.303153738343295E-5	0.0	0.009423906686971815	0.0
10	0.0	4.303153738343295E-5	0.0	0.014415565023450037	0.0
11	0.0	4.303153738343295E-5	0.0	0.01635198420570452	0.0
12	0.0	8.60630747668659E-5	0.0	0.01764293032720751	0.0
13	0.0	8.60630747668659E-5	0.0	0.018890844911327063	0.0
14	0.0	8.60630747668659E-5	0.0	0.019923601808529454	0.0
15	0.0	8.60630747668659E-5	0.0	0.0222042732898514	0.0
16	0.0	8.60630747668659E-5	0.0	0.024484944771173345	0.0
17	0.0	1.2909461215029883E-4	0.0	0.027583215462780517	0.0
18	0.0	1.721261495337318E-4	0.0	0.029734792331952163	0.0
19	0.0	1.721261495337318E-4	0.0	0.03210152688804098	0.0
20	0.0	1.721261495337318E-4	0.0	0.03455432451889665	0.0
21	0.0	1.721261495337318E-4	0.0	0.03868535210770622	0.0
22	0.0	1.721261495337318E-4	0.0	0.0446667358040034	0.0
23	0.0	1.721261495337318E-4	0.0	0.05348820096760715	0.0
24	0.0	1.721261495337318E-4	0.0	0.06562309450973523	0.0
25	0.0	1.721261495337318E-4	0.0	0.06919471211256017	0.0
26	0.0	1.721261495337318E-4	0.0	0.07685432576681124	0.0
27	0.0	1.721261495337318E-4	0.0	0.09032319696782574	0.0
28	0.0	1.721261495337318E-4	0.0	0.12091862004744658	0.0
29	0.0	2.1515768691716472E-4	0.0	0.1647247251037813	0.0
30	0.0	2.1515768691716472E-4	0.0	0.22755076968359342	0.0
31	0.0	2.1515768691716472E-4	0.0	0.45708099008682473	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
AAGACGG	365	0.0	18.753424	5
TATACTG	180	0.0	17.472221	5
CGCAAGA	385	0.0	17.298702	2
CGGACCA	370	0.0	17.000002	9
ACGAACG	210	0.0	16.738094	15
GACGGAC	365	0.0	16.726027	7
GCTACGA	100	5.88299E-6	16.650002	2
GGTATCA	3285	0.0	16.388126	1
CGAACGA	205	0.0	16.243904	16
GCGTTAT	300	0.0	15.416667	1
TCTAGCG	230	0.0	15.282609	28
TCGCCAT	860	0.0	15.273255	13
CGAACTA	135	3.9769293E-7	15.074075	24
ATTGCCG	310	0.0	14.919355	11
ATCTCGC	960	0.0	14.838542	11
TAACGAA	225	1.8189894E-12	14.8	13
CTAGTAC	75	0.0041060834	14.8	3
ACGGACC	425	0.0	14.8	8
CTTATAC	1645	0.0	14.732522	37
CTAGCGG	240	0.0	14.645834	29
>>END_MODULE