FastQCFastQC Report
Fri 10 Feb 2017
ERR1630590.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1630590.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1193704
Sequences flagged as poor quality0
Sequence length43
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTGTAGCGCGCGTGCAGCCCCGGACATCTAAGGGCATCACAGA12730.10664285283453853No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTTACCG358.8695966E-426.428579
CGTCTAT450.00382589320.5555551
CAAGACG2250.018.9111124
CTAGACT902.1523574E-618.54
ACTTACC609.2371006E-418.58
ATAACGA1700.018.512
CGAACGA1552.0008883E-1117.90322516
TATACTG1156.405571E-817.6956525
GGTATCA6800.017.6838251
AAGACCG852.7232572E-517.4117645
CGTATAT752.0673509E-417.26666610
TAACGAA1655.4569682E-1116.81818213
AGTCGGT2100.016.73809611
TCTATGG3250.016.5076922
TCGTTTA1708.54925E-1116.3235330
ATGCGGC2300.016.08695635
TACGACG2200.015.9772735
TATACAG1059.343619E-615.8571445
ACGAACG1751.3096724E-1015.85714315
CGCCTTA1802.0190782E-1015.41666625
Produced by FastQC (version 0.11.5)