FastQCFastQC Report
Fri 10 Feb 2017
ERR1630589.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1630589.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2039563
Sequences flagged as poor quality0
Sequence length43
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT105730.5183953621437534No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT103210.5060397742065335No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT91000.4461740088440514No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT60340.29584768894120944No Hit
GATATGGACAATACTAGTTAAGAAAGCTAACAGGATGTGAAAG42300.20739736894619093No Hit
GTCCCAGGCTGCTGAGCAGGATGAAATGAGGCTTGAGCTGCAG32430.15900464952541304No Hit
GTCCATATCAGACCTCTGATCCCTCGCCCCCACACCCCATCTC31390.15390551799576674No Hit
GCCTGGGACAGATCTTCAGGTTCCAGGGCATCATTCTCCGTCT31380.15385648788490477No Hit
GCCCAGGGCCAGGACGATGGACAGCGCAGCCAGCGCGCACTGG28960.14199120105630472No Hit
GTCTGAACCCAACCAGACGGAGAATGATGCCCTGGAACCTGAA26650.1306652454471865No Hit
GGATGAAATGAGGCTTGAGCTGCAGAGATCTGCTAACTCAAAC25240.12375199981564679No Hit
AGCTAACAGGATGTGAAAGTCTTCCAGAAGAAATTCTTGCAGC24560.12041795227703189No Hit
GCCCTGGGCTGTGTCACCGGCGCTCCCTCGGACCCCAGACTCC22320.10943520744394755No Hit
GGACAATACTAGTTAAGAAAGCTAACAGGATGTGAAAGTCTTC20470.10036463693448058No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGTAAG500.00703670618.4999982
TATAGAC1002.8776412E-718.4999983
AAGACGG2950.018.1864415
ACCGTCC752.0682564E-417.2666668
CGCAAGC650.001580500917.07692512
ACTAGAC904.4486747E-516.4444453
AGTCGGT2900.015.94827611
GGTATCA53050.015.2742691
TACCGTC1853.0559022E-1015.07
TAATACT4600.014.8804354
CCGTCCA1001.0940236E-414.7999999
CCGGTCC2750.014.7999999
GCAGTCG3400.014.6911759
CGGACCA3150.014.6825399
TAGTTCG1406.0009006E-714.5357157
CGGTCCA2800.014.53571510
CCGGTCG2353.6379788E-1214.17021220
CTTATAC16300.014.07361937
CAGTCGG3350.013.8059710
CGCCTTA2950.013.7966125
Produced by FastQC (version 0.11.5)