##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1630589.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	2039563
Sequences flagged as poor quality	0
Sequence length	43
%GC	48
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.05988243560017	33.0	31.0	34.0	30.0	34.0
2	32.2205428319694	33.0	31.0	34.0	30.0	34.0
3	32.32679892702505	34.0	31.0	34.0	30.0	34.0
4	35.91139229334912	37.0	35.0	37.0	35.0	37.0
5	35.87776401121221	37.0	35.0	37.0	35.0	37.0
6	35.95486827325265	37.0	35.0	37.0	35.0	37.0
7	35.89415428697226	37.0	35.0	37.0	35.0	37.0
8	35.90207510138201	37.0	35.0	37.0	35.0	37.0
9	37.62040986230874	39.0	37.0	39.0	35.0	39.0
10	37.52472563975714	39.0	37.0	39.0	35.0	39.0
11	37.626439585342546	39.0	37.0	39.0	35.0	39.0
12	37.564934743373946	39.0	37.0	39.0	35.0	39.0
13	37.613630468879855	39.0	37.0	39.0	35.0	39.0
14	38.907264448315644	40.0	38.0	41.0	36.0	41.0
15	38.90738751389391	40.0	38.0	41.0	36.0	41.0
16	38.87666965913777	40.0	38.0	41.0	35.0	41.0
17	38.858719245250086	40.0	38.0	41.0	35.0	41.0
18	38.84994040390025	40.0	38.0	41.0	35.0	41.0
19	38.91904981606354	40.0	38.0	41.0	35.0	41.0
20	38.897870769375594	40.0	38.0	41.0	35.0	41.0
21	38.850509153186245	40.0	38.0	41.0	35.0	41.0
22	38.783599231796224	40.0	38.0	41.0	35.0	41.0
23	38.733800819097034	40.0	38.0	41.0	35.0	41.0
24	38.67807368539241	40.0	38.0	41.0	34.0	41.0
25	38.63035807180264	40.0	38.0	41.0	34.0	41.0
26	38.48915331372456	40.0	38.0	41.0	34.0	41.0
27	38.36445307156484	40.0	38.0	41.0	34.0	41.0
28	38.263483403062324	40.0	38.0	41.0	34.0	41.0
29	38.180452871521986	40.0	38.0	41.0	34.0	41.0
30	38.085915463263454	40.0	37.0	41.0	33.0	41.0
31	38.00866754299818	40.0	37.0	41.0	33.0	41.0
32	37.9059764273033	40.0	37.0	41.0	33.0	41.0
33	37.82198980860116	40.0	37.0	41.0	33.0	41.0
34	37.73240591244301	40.0	37.0	41.0	33.0	41.0
35	37.66766263165198	40.0	37.0	41.0	33.0	41.0
36	37.606167105404445	40.0	37.0	41.0	33.0	41.0
37	37.4544870641407	40.0	36.0	41.0	32.0	41.0
38	37.3103061783333	40.0	36.0	41.0	32.0	41.0
39	37.20892906960952	40.0	36.0	41.0	32.0	41.0
40	37.01099941507078	40.0	36.0	41.0	31.0	41.0
41	36.930155626474885	39.0	35.0	41.0	31.0	41.0
42	36.77241889561636	39.0	35.0	41.0	31.0	41.0
43	35.996256551035685	38.0	35.0	40.0	29.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
9	5.0
10	7.0
11	7.0
12	5.0
13	0.0
14	1.0
15	6.0
16	9.0
17	24.0
18	44.0
19	105.0
20	241.0
21	457.0
22	886.0
23	1468.0
24	2602.0
25	4302.0
26	6772.0
27	10221.0
28	15066.0
29	20762.0
30	28440.0
31	37729.0
32	48658.0
33	63087.0
34	82962.0
35	110673.0
36	158817.0
37	254654.0
38	445068.0
39	746485.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	45.317550867514264	19.16964565448579	11.62582376715012	23.886979710849822
2	18.737200076683095	21.295885442126572	34.91522448681409	25.051689994376247
3	20.931395597978586	23.530089533885445	28.497133944869564	27.041380923266406
4	13.54554872784023	15.437767796336765	34.606923149713936	36.40976032610907
5	14.414803563312336	37.4484632247202	33.55767877726748	14.579054434699982
6	35.13571289536043	34.24253136578767	16.05976378273189	14.561991956120012
7	29.769465321738036	30.439657907110494	21.102265534332602	18.68861123681887
8	27.82336216140418	32.58286211310952	20.234726752740663	19.359048972745633
9	26.887573465492366	13.00724714068651	20.360881227988546	39.74429816583258
10	17.052966738463095	26.898605240436307	32.87920010315935	23.169227917941246
11	35.261229979167105	22.117532039951694	21.062305993980083	21.558931986901115
12	21.847866430210786	25.745809273849353	29.252344742476698	23.15397955346317
13	30.06869608832873	20.02291667381689	25.894615660315466	24.013771577538915
14	23.07175605754762	21.30961387316793	25.968651127717063	29.649978941567383
15	25.335280155601957	27.641068209219327	23.956357317719533	23.06729431745918
16	25.569644085522242	25.94501861428159	24.73990751940489	23.745429780791277
17	23.269151283877967	26.30970457887302	25.90456877282045	24.516575364428554
18	22.652597639788524	24.474017228200353	28.428246639108472	24.445138492902647
19	24.288585348920332	25.640100354830913	27.63469429480727	22.436620001441483
20	24.6738639600738	24.796046996341865	27.69848246903871	22.831606574545628
21	24.59379778903618	25.263107832413116	26.81314575720387	23.329948621346826
22	25.112683452288554	24.370171453394672	27.051579186325696	23.465565907991074
23	22.926675959507012	25.04428644763609	27.689411898529244	24.33962569432766
24	23.34652079881818	26.082842255914624	27.070798989783597	23.499837955483603
25	23.560488202619876	25.593129508625132	27.22347875500781	23.62290353374718
26	24.254068150873493	25.840829628699876	26.47743658813187	23.42766563229476
27	24.238525605730246	25.204222669267878	26.3061253807801	24.251126344221777
28	23.27459362618365	25.51183758481596	27.688039055425108	23.525529733575283
29	23.285478310795007	26.22453927630576	27.891317895058894	22.59866451784034
30	23.213698228493065	25.977133336896184	27.02971175688125	23.779456677729492
31	22.87661621631693	25.714430002897682	27.248484111547423	24.16046966923797
32	22.202550252186377	26.18855117493306	27.274911341302033	24.33398723157853
33	22.840677145055093	25.240652041638334	27.61684733445351	24.30182347885307
34	23.899335298787044	25.52924327417197	27.664945873209113	22.906475553831875
35	23.721306966247184	25.529096183839386	26.993772685619422	23.75582416429402
36	22.43691418210666	26.079115967489113	26.525437066665752	24.958532783738477
37	22.721828156325643	25.37519066584362	28.22187890249039	23.681102275340354
38	22.35272948175663	24.70749861612512	27.821400956969704	25.118370945148545
39	22.427745551375466	25.253694051127617	28.65800173860773	23.660558658889183
40	22.848129721906112	24.934949300413862	28.286892829493375	23.930028148186647
41	21.574964833152986	25.501394171202364	28.83544170981725	24.088199285827404
42	21.761083133985075	26.019103111794045	28.59048727595078	23.629326478270098
43	22.014666867363253	24.317120873442008	28.19829541916577	25.469916840028965
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	344.0
1	397.0
2	450.0
3	1330.0
4	2210.0
5	2210.0
6	3137.5
7	4065.0
8	4001.5
9	3938.0
10	5358.5
11	6779.0
12	6779.0
13	11286.0
14	15793.0
15	20894.0
16	25995.0
17	25007.0
18	24019.0
19	24019.0
20	26429.0
21	28839.0
22	26381.0
23	23923.0
24	26681.5
25	29440.0
26	29440.0
27	32436.0
28	35432.0
29	40289.0
30	45146.0
31	50621.5
32	56097.0
33	56097.0
34	69422.5
35	82748.0
36	88777.5
37	94807.0
38	97909.5
39	101012.0
40	101012.0
41	102954.5
42	104897.0
43	111993.0
44	119089.0
45	118662.0
46	118235.0
47	118235.0
48	120145.0
49	122055.0
50	126610.0
51	131165.0
52	138900.0
53	146635.0
54	146635.0
55	146140.5
56	145646.0
57	134190.0
58	122734.0
59	120291.0
60	117848.0
61	117848.0
62	107707.5
63	97567.0
64	80951.5
65	64336.0
66	57420.5
67	50505.0
68	50505.0
69	44694.0
70	38883.0
71	37732.5
72	36582.0
73	30748.0
74	24914.0
75	24914.0
76	15764.0
77	6614.0
78	5268.0
79	3922.0
80	3265.5
81	2609.0
82	2609.0
83	2096.5
84	1584.0
85	1364.5
86	1145.0
87	1084.5
88	1024.0
89	1024.0
90	741.0
91	458.0
92	259.5
93	61.0
94	37.5
95	14.0
96	14.0
97	8.5
98	3.0
99	2.0
100	1.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	2039563.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	54.84202885611986
#Duplication Level	Percentage of deduplicated	Percentage of total
1	87.89855816571416	48.20535263335427
2	7.794846753750191	8.549704211964006
3	1.7167301510879358	2.8244689345240683
4	0.6862717369096449	1.50546137594953
5	0.35448218177173985	0.9720261020853043
6	0.24439717746780487	0.8041942235426163
7	0.16982465011075315	0.6519469853298069
8	0.11842475708321741	0.5195723156189436
9	0.0924382193054326	0.4562549541501183
>10	0.7143943146364259	7.836253396373961
>50	0.09407374794435149	3.627006139491536
>100	0.09418966303998275	10.804161016001903
>500	0.013557313688846823	5.065916780851402
>1k	0.007452033352147591	6.403694209984715
>5k	1.7956706872644798E-4	0.7451847183372061
>10k+	1.7956706872644798E-4	1.028802002440702
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	10573	0.5183953621437534	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	10321	0.5060397742065335	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	9100	0.4461740088440514	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	6034	0.29584768894120944	No Hit
GATATGGACAATACTAGTTAAGAAAGCTAACAGGATGTGAAAG	4230	0.20739736894619093	No Hit
GTCCCAGGCTGCTGAGCAGGATGAAATGAGGCTTGAGCTGCAG	3243	0.15900464952541304	No Hit
GTCCATATCAGACCTCTGATCCCTCGCCCCCACACCCCATCTC	3139	0.15390551799576674	No Hit
GCCTGGGACAGATCTTCAGGTTCCAGGGCATCATTCTCCGTCT	3138	0.15385648788490477	No Hit
GCCCAGGGCCAGGACGATGGACAGCGCAGCCAGCGCGCACTGG	2896	0.14199120105630472	No Hit
GTCTGAACCCAACCAGACGGAGAATGATGCCCTGGAACCTGAA	2665	0.1306652454471865	No Hit
GGATGAAATGAGGCTTGAGCTGCAGAGATCTGCTAACTCAAAC	2524	0.12375199981564679	No Hit
AGCTAACAGGATGTGAAAGTCTTCCAGAAGAAATTCTTGCAGC	2456	0.12041795227703189	No Hit
GCCCTGGGCTGTGTCACCGGCGCTCCCTCGGACCCCAGACTCC	2232	0.10943520744394755	No Hit
GGACAATACTAGTTAAGAAAGCTAACAGGATGTGAAAGTCTTC	2047	0.10036463693448058	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	4.903011086198367E-5	0.0
2	0.0	0.0	0.0	8.335118846537223E-4	0.0
3	0.0	0.0	0.0	0.0012257527715495917	0.0
4	0.0	0.0	0.0	0.0021573248779272816	0.0
5	0.0	0.0	0.0	0.0025985958756851346	0.0
6	0.0	0.0	0.0	0.002647625986547118	0.0
7	0.0	0.0	0.0	0.0029908367625810037	0.0
8	0.0	0.0	0.0	0.003285017427752906	0.0
9	0.0	0.0	0.0	0.005442342305680187	0.0
10	0.0	0.0	0.0	0.008335118846537225	0.0
11	0.0	0.0	0.0	0.0096099017289488	0.0
12	0.0	0.0	0.0	0.010982744833084342	0.0
13	0.0	0.0	0.0	0.011669166385152113	0.0
14	0.0	0.0	0.0	0.013042009489287656	0.0
15	0.0	0.0	0.0	0.01456194292600915	0.0
16	0.0	0.0	0.0	0.018925622792725697	0.0
17	0.0	0.0	0.0	0.025446627537369523	0.0
18	0.0	4.903011086198367E-5	0.0	0.02843746429995053	0.0
19	0.0	4.903011086198367E-5	0.0	0.03397786682735468	0.0
20	0.0	4.903011086198367E-5	0.0	0.036919673479073704	0.0
21	0.0	4.903011086198367E-5	0.0	0.04368582877802745	0.0
22	0.0	4.903011086198367E-5	0.0	0.05594335649352337	0.0
23	0.0	9.806022172396734E-5	0.0	0.07810496660313998	0.0
24	0.0	9.806022172396734E-5	0.0	0.10919005688963764	0.0
25	0.0	9.806022172396734E-5	0.0	0.11777032629048477	0.0
26	0.0	9.806022172396734E-5	0.0	0.13233226921649394	0.0
27	0.0	9.806022172396734E-5	0.0	0.15155207267439152	0.0
28	0.0	9.806022172396734E-5	0.0	0.19180579369208012	0.0
29	0.0	9.806022172396734E-5	0.0	0.2482884814050853	0.0
30	0.0	9.806022172396734E-5	0.0	0.32953137510339225	0.0
31	0.0	9.806022172396734E-5	0.0	0.6501392700299035	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TCGTAAG	50	0.007036706	18.499998	2
TATAGAC	100	2.8776412E-7	18.499998	3
AAGACGG	295	0.0	18.186441	5
ACCGTCC	75	2.0682564E-4	17.266666	8
CGCAAGC	65	0.0015805009	17.076925	12
ACTAGAC	90	4.4486747E-5	16.444445	3
AGTCGGT	290	0.0	15.948276	11
GGTATCA	5305	0.0	15.274269	1
TACCGTC	185	3.0559022E-10	15.0	7
TAATACT	460	0.0	14.880435	4
CCGTCCA	100	1.0940236E-4	14.799999	9
CCGGTCC	275	0.0	14.799999	9
GCAGTCG	340	0.0	14.691175	9
CGGACCA	315	0.0	14.682539	9
TAGTTCG	140	6.0009006E-7	14.535715	7
CGGTCCA	280	0.0	14.535715	10
CCGGTCG	235	3.6379788E-12	14.170212	20
CTTATAC	1630	0.0	14.073619	37
CAGTCGG	335	0.0	13.80597	10
CGCCTTA	295	0.0	13.79661	25
>>END_MODULE