FastQCFastQC Report
Fri 10 Feb 2017
ERR1630588.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1630588.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1507893
Sequences flagged as poor quality0
Sequence length43
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT33820.22428647125492326No Hit
ACCTAGGCCTGGTCAGCATGTTGATGTATCTACGGAGATCAGC33820.22428647125492326No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT32580.2160630760935955No Hit
GGCCTAGGTATGGGAAAAGACACAAAGAGGACACGCTGGCCTT28420.18847491168139915No Hit
GCTTTATTGAGCCTGTGTGGGAGCAGGGAGCAAGCTTTGGCCA27490.1823073653104033No Hit
GTGTGGGAGCAGGGAGCAAGCTTTGGCCAGAGCCAAGGGTGCA26190.17368606393159197No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT24980.165661621879006No Hit
GTATGCAGCTGATCTCCGTAGATACATCAACATGCTGACCAGG21520.142715696670785No Hit
GGGTACACTGGCTCCAGTGGGGCTCCCTGGGCACCCAGCAGTG20470.135752337864822No Hit
AGTGTACCCAGGGGACAATGCCACACCAGAGCAGATGGCCCAG20340.13489020772694085No Hit
GCTGCATACTGGGCCATCTGCTCTGGTGTGGCATTGTCCCCTG19860.13170695798707205No Hit
CCTCTGCCTCTCCCTGCTGCTCCTGTCCACCTGCGTGGCTCTG19040.12626890634812946No Hit
GTCTAGTGCCCATTTACTCTGGACTCCGGATGGCTGCCGCACG16990.1126737772507731No Hit
GCTCATGGAGTCGTAGGAGACAGAAGGTGGCATTATAAGTCCA16770.11121478778666656No Hit
GGGGAGAGCTGGGCTGGCGCTGCTCATGGAGTCGTAGGAGACA16520.10955684521381823No Hit
GTGCAGAGGGGAGAGCTGGGCTGGCGCTGCTCATGGAGTCGTA16280.10796522034388382No Hit
CTGTTACTACAGCCACTGCTGGGTGCCCAGGGAGCCCCACTGG15730.10431774668361747No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT15340.10173135626997407No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATACCCG555.1441154E-420.1818185
CCGTGTA609.238635E-418.59
ACCGTGT1002.8764043E-718.58
GCTTTAT7650.017.6535951
GGTATCA22550.016.9822621
TTATACT2100.016.7380964
TATACTT2000.016.6500025
CGATAAC1005.880236E-616.65000210
ATATACT1801.0913936E-1116.4444454
TAACGAA1151.2427463E-616.08695613
TAAGACG700.00259299715.8571424
TATACTG2350.015.7446815
CGAACGA1101.4523861E-515.13636416
ACGAACG1101.4523861E-515.13636415
ATAACGA1353.9742372E-715.07407312
TATTGAG8600.015.0581395
CGTGCAT750.00410519614.810
TCTTATA9500.014.837
TCCGATA1252.9600433E-614.7999998
TCGTTTA1152.2106367E-514.47826130
Produced by FastQC (version 0.11.5)