##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1630588.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1507893
Sequences flagged as poor quality	0
Sequence length	43
%GC	51
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.767356171823863	31.0	31.0	34.0	30.0	34.0
2	31.945127406254954	33.0	31.0	34.0	30.0	34.0
3	32.05100892437328	33.0	31.0	34.0	30.0	34.0
4	35.69477608822377	37.0	35.0	37.0	33.0	37.0
5	35.62612333899023	37.0	35.0	37.0	33.0	37.0
6	35.69548170858277	37.0	35.0	37.0	33.0	37.0
7	35.648059908760104	37.0	35.0	37.0	33.0	37.0
8	35.64213773788989	37.0	35.0	37.0	33.0	37.0
9	37.3081816813262	39.0	37.0	39.0	34.0	39.0
10	37.1717243862794	39.0	37.0	39.0	33.0	39.0
11	37.281054424949254	39.0	37.0	39.0	34.0	39.0
12	37.21600073745286	39.0	37.0	39.0	34.0	39.0
13	37.272804502706755	39.0	37.0	39.0	34.0	39.0
14	38.49182866423546	40.0	38.0	41.0	34.0	41.0
15	38.50091021047249	40.0	38.0	41.0	34.0	41.0
16	38.45364226772059	40.0	38.0	41.0	34.0	41.0
17	38.43037536483026	40.0	38.0	41.0	34.0	41.0
18	38.40691614060149	40.0	38.0	41.0	34.0	41.0
19	38.43992909311204	40.0	38.0	41.0	34.0	41.0
20	38.41338012710451	40.0	38.0	41.0	34.0	41.0
21	38.35434742385567	40.0	38.0	41.0	34.0	41.0
22	38.29477887356729	40.0	38.0	41.0	34.0	41.0
23	38.256033418816855	40.0	38.0	41.0	34.0	41.0
24	38.18596080756393	40.0	38.0	41.0	33.0	41.0
25	38.1328774654435	40.0	38.0	41.0	33.0	41.0
26	37.98725771656212	40.0	37.0	41.0	33.0	41.0
27	37.84649772895026	40.0	37.0	41.0	33.0	41.0
28	37.74051607110054	40.0	37.0	41.0	33.0	41.0
29	37.67477334267087	40.0	37.0	41.0	32.0	41.0
30	37.58478817794101	40.0	37.0	41.0	32.0	41.0
31	37.54201325956152	40.0	37.0	41.0	32.0	41.0
32	37.47169792551593	40.0	37.0	41.0	32.0	41.0
33	37.39039971669077	39.0	36.0	41.0	32.0	41.0
34	37.3344978721965	39.0	36.0	41.0	31.0	41.0
35	37.28028116053327	39.0	36.0	41.0	31.0	41.0
36	37.267519644961546	39.0	36.0	41.0	31.0	41.0
37	37.13120758568413	39.0	36.0	41.0	31.0	41.0
38	36.99531664382022	39.0	36.0	41.0	31.0	41.0
39	36.932633814202994	39.0	36.0	40.0	31.0	41.0
40	36.75043520992537	39.0	35.0	40.0	30.0	41.0
41	36.71588700259236	39.0	35.0	40.0	30.0	41.0
42	36.588642562834366	39.0	35.0	40.0	30.0	41.0
43	35.72109227909407	38.0	34.0	40.0	28.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	1.0
9	1.0
10	4.0
11	1.0
12	7.0
13	2.0
14	3.0
15	7.0
16	10.0
17	20.0
18	39.0
19	101.0
20	204.0
21	425.0
22	826.0
23	1473.0
24	2421.0
25	4007.0
26	6321.0
27	9176.0
28	13168.0
29	18220.0
30	24526.0
31	31945.0
32	41666.0
33	54069.0
34	71650.0
35	96421.0
36	136543.0
37	216073.0
38	403828.0
39	374735.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	44.669150927817824	18.223706854531454	11.51573752248999	25.59140469516073
2	20.37233411123999	20.202295520968665	32.06494094740144	27.36042942038991
3	21.962632627116115	21.873236363588134	28.097020146654966	28.06711086264078
4	15.594674157914387	14.365939758325027	33.103409857330725	36.935976226429865
5	15.581808523549084	35.79239375738199	33.68985730419864	14.935940414870286
6	37.765080148259855	32.83130832227486	14.301810539607255	15.101800989858033
7	30.913731942518467	28.841701632675527	20.23797444513636	20.006591979669643
8	29.486243387296046	30.827253657918696	19.08543908619511	20.601063868590145
9	27.4698536301979	13.01113540549628	18.647344340745665	40.87166662356016
10	18.088684011398687	25.88439630663449	31.85179585023606	24.175123831730765
11	37.433557951393105	20.682634643174282	20.189960428226673	21.69384697720594
12	24.087252875369806	24.314258372444197	27.05762278888489	24.54086596330111
13	32.372389818110435	18.718171647457744	23.501866511748513	25.407572022683304
14	24.073591428569536	19.604043522982067	23.8644917112819	32.4578733371665
15	27.175601982368775	26.744802184239862	21.00666293961176	25.072932893779598
16	26.557985215131314	25.035065485415743	22.756322895590074	25.650626403862876
17	25.55479732315224	24.83710714221765	23.771978515716963	25.836117018913146
18	24.95143886204127	23.704931318070976	25.693467639945272	25.650162179942477
19	26.57781420830258	23.69160145978528	25.484500558063473	24.246083773848675
20	27.096418645089535	23.132410588814988	24.38475409064171	25.38641667545376
21	26.58338489534735	23.407496420501985	24.755801638445167	25.253317045705497
22	26.678948705246324	23.70871142713707	24.730468275932047	24.881871591684558
23	25.93081869867424	23.655458311697185	24.314391007850027	26.099331981778544
24	25.62210979160988	24.12783930955313	25.02670945484859	25.2233414439884
25	26.04203348646091	24.213389146312107	24.099654285814708	25.644923081412273
26	26.021541316260503	24.266310673237424	24.44749063759829	25.26465737290378
27	26.050853740948465	23.153101712124137	24.6612325940899	26.1348119528375
28	25.548629776781244	24.02212889110832	24.47123237524148	25.958008956868955
29	25.681198864906197	24.596904422263382	24.92511073398444	24.79678597884598
30	25.949918197113455	24.146275630963203	24.168359426033543	25.735446745889796
31	24.97889439104764	24.985725114447778	24.916224161793973	25.119156332710613
32	24.986255656071087	24.0881813232106	24.607913160947096	26.31764985977122
33	24.396823912572046	24.82530259109897	25.014374362106594	25.76349913422239
34	26.514679755128512	23.268560832897293	24.61859031111624	25.598169100857955
35	25.175990604107852	23.690606694241566	24.92829398372431	26.205108717926272
36	24.65519768312473	24.678010972927126	25.290720230148956	25.37607111379919
37	25.39749173184039	23.34993265437269	25.67496500083229	25.577610612954633
38	25.5084412488154	23.824104230207315	24.99421378042076	25.673240740556523
39	25.087522788420664	23.554854356376744	25.566999780488402	25.790623074714187
40	24.328052454650297	23.13426748449658	26.075391291026616	26.462288769826507
41	23.339984998935602	23.704400776447663	26.6355106098377	26.320103614779033
42	24.0477275244331	23.33149633296262	27.163996384358835	25.456779758245446
43	24.619120852739552	23.13711914572188	26.41832013279457	25.825439868744006
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	49.0
1	70.5
2	92.0
3	241.0
4	390.0
5	390.0
6	626.0
7	862.0
8	844.0
9	826.0
10	1171.0
11	1516.0
12	1516.0
13	2716.5
14	3917.0
15	5736.0
16	7555.0
17	7328.0
18	7101.0
19	7101.0
20	8608.0
21	10115.0
22	9901.5
23	9688.0
24	11670.5
25	13653.0
26	13653.0
27	16198.0
28	18743.0
29	22265.0
30	25787.0
31	30022.0
32	34257.0
33	34257.0
34	38985.5
35	43714.0
36	49111.0
37	54508.0
38	59774.5
39	65041.0
40	65041.0
41	68638.5
42	72236.0
43	75417.0
44	78598.0
45	82385.0
46	86172.0
47	86172.0
48	92885.5
49	99599.0
50	101365.0
51	103131.0
52	104812.0
53	106493.0
54	106493.0
55	103933.0
56	101373.0
57	101510.0
58	101647.0
59	105079.0
60	108511.0
61	108511.0
62	100729.0
63	92947.0
64	87036.0
65	81125.0
66	71566.0
67	62007.0
68	62007.0
69	55222.5
70	48438.0
71	40647.0
72	32856.0
73	24780.0
74	16704.0
75	16704.0
76	12288.5
77	7873.0
78	6263.5
79	4654.0
80	3705.0
81	2756.0
82	2756.0
83	2082.0
84	1408.0
85	1120.0
86	832.0
87	644.5
88	457.0
89	457.0
90	336.5
91	216.0
92	123.0
93	30.0
94	21.5
95	13.0
96	13.0
97	7.5
98	2.0
99	1.5
100	1.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	1507893.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	60.980084074414144
#Duplication Level	Percentage of deduplicated	Percentage of total
1	86.7431149109186	52.89602440144384
2	8.629401303523975	10.52443234001502
3	2.0504590198793475	3.7511149027015023
4	0.7873058068419099	1.920398971739765
5	0.40150936937954007	1.2242037550714677
6	0.26125365065953965	0.955876174917981
7	0.1600512981311916	0.683195913138143
8	0.119966025840647	0.5852430673468795
9	0.0955335273607329	0.5243078277344552
>10	0.5960268656327495	7.1181849949947775
>50	0.06954950244763274	3.021137366461896
>100	0.07361432213820777	9.227492438753815
>500	0.008506455224824168	3.6208955057666454
>1k	0.0037079420210772017	3.9474923399139814
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	3382	0.22428647125492326	No Hit
ACCTAGGCCTGGTCAGCATGTTGATGTATCTACGGAGATCAGC	3382	0.22428647125492326	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	3258	0.2160630760935955	No Hit
GGCCTAGGTATGGGAAAAGACACAAAGAGGACACGCTGGCCTT	2842	0.18847491168139915	No Hit
GCTTTATTGAGCCTGTGTGGGAGCAGGGAGCAAGCTTTGGCCA	2749	0.1823073653104033	No Hit
GTGTGGGAGCAGGGAGCAAGCTTTGGCCAGAGCCAAGGGTGCA	2619	0.17368606393159197	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	2498	0.165661621879006	No Hit
GTATGCAGCTGATCTCCGTAGATACATCAACATGCTGACCAGG	2152	0.142715696670785	No Hit
GGGTACACTGGCTCCAGTGGGGCTCCCTGGGCACCCAGCAGTG	2047	0.135752337864822	No Hit
AGTGTACCCAGGGGACAATGCCACACCAGAGCAGATGGCCCAG	2034	0.13489020772694085	No Hit
GCTGCATACTGGGCCATCTGCTCTGGTGTGGCATTGTCCCCTG	1986	0.13170695798707205	No Hit
CCTCTGCCTCTCCCTGCTGCTCCTGTCCACCTGCGTGGCTCTG	1904	0.12626890634812946	No Hit
GTCTAGTGCCCATTTACTCTGGACTCCGGATGGCTGCCGCACG	1699	0.1126737772507731	No Hit
GCTCATGGAGTCGTAGGAGACAGAAGGTGGCATTATAAGTCCA	1677	0.11121478778666656	No Hit
GGGGAGAGCTGGGCTGGCGCTGCTCATGGAGTCGTAGGAGACA	1652	0.10955684521381823	No Hit
GTGCAGAGGGGAGAGCTGGGCTGGCGCTGCTCATGGAGTCGTA	1628	0.10796522034388382	No Hit
CTGTTACTACAGCCACTGCTGGGTGCCCAGGGAGCCCCACTGG	1573	0.10431774668361747	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1534	0.10173135626997407	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	8.621301378811361E-4	0.0
3	0.0	0.0	0.0	0.0017905779786762058	0.0
4	0.0	0.0	0.0	0.003382202848610611	0.0
5	2.6527081165573417E-4	0.0	0.0	0.003846426769008146	0.0
6	2.6527081165573417E-4	0.0	0.0	0.00391274447192208	0.0
7	2.6527081165573417E-4	0.0	0.0	0.004642239203975348	0.0
8	2.6527081165573417E-4	0.0	0.0	0.005239098530200751	0.0
9	2.6527081165573417E-4	0.0	0.0	0.00822339516132776	0.0
10	3.3158851456966776E-4	0.0	0.0	0.012666681256561308	0.0
11	4.642239203975348E-4	0.0	0.0	0.01366144680027031	0.0
12	4.642239203975348E-4	0.0	0.0	0.014457259235237514	0.0
13	4.642239203975348E-4	0.0	0.0	0.014855165452721114	0.0
14	4.642239203975348E-4	0.0	0.0	0.015849930996430117	0.0
15	5.96859326225402E-4	0.0	0.0	0.017242602757622724	0.0
16	5.96859326225402E-4	0.0	0.0	0.019895310874180065	0.0
17	5.96859326225402E-4	0.0	0.0	0.023144878316962807	6.631770291393354E-5
18	5.96859326225402E-4	0.0	0.0	0.024935456295639015	6.631770291393354E-5
19	5.96859326225402E-4	0.0	0.0	0.026858669680143086	6.631770291393354E-5
20	5.96859326225402E-4	0.0	0.0	0.02951137779670043	6.631770291393354E-5
21	7.29494732053269E-4	0.0	0.0	0.033357804565708575	6.631770291393354E-5
22	7.29494732053269E-4	0.0	0.0	0.03959166863961833	6.631770291393354E-5
23	7.29494732053269E-4	0.0	0.0	0.048080334612601826	6.631770291393354E-5
24	7.29494732053269E-4	0.0	0.0	0.060150156542937726	6.631770291393354E-5
25	7.29494732053269E-4	0.0	0.0	0.06366499479737621	6.631770291393354E-5
26	7.29494732053269E-4	0.0	0.0	0.06837355170426548	6.631770291393354E-5
27	7.29494732053269E-4	0.0	0.0	0.07580113443062604	6.631770291393354E-5
28	8.621301378811361E-4	0.0	0.0	0.09629330463103151	6.631770291393354E-5
29	8.621301378811361E-4	0.0	0.0	0.1279268489209778	6.631770291393354E-5
30	9.284478407950696E-4	0.0	0.0	0.1729565691995387	6.631770291393354E-5
31	9.284478407950696E-4	0.0	0.0	0.334307540389139	6.631770291393354E-5
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
ATACCCG	55	5.1441154E-4	20.181818	5
CCGTGTA	60	9.238635E-4	18.5	9
ACCGTGT	100	2.8764043E-7	18.5	8
GCTTTAT	765	0.0	17.653595	1
GGTATCA	2255	0.0	16.982262	1
TTATACT	210	0.0	16.738096	4
TATACTT	200	0.0	16.650002	5
CGATAAC	100	5.880236E-6	16.650002	10
ATATACT	180	1.0913936E-11	16.444445	4
TAACGAA	115	1.2427463E-6	16.086956	13
TAAGACG	70	0.002592997	15.857142	4
TATACTG	235	0.0	15.744681	5
CGAACGA	110	1.4523861E-5	15.136364	16
ACGAACG	110	1.4523861E-5	15.136364	15
ATAACGA	135	3.9742372E-7	15.074073	12
TATTGAG	860	0.0	15.058139	5
CGTGCAT	75	0.004105196	14.8	10
TCTTATA	950	0.0	14.8	37
TCCGATA	125	2.9600433E-6	14.799999	8
TCGTTTA	115	2.2106367E-5	14.478261	30
>>END_MODULE