FastQCFastQC Report
Fri 10 Feb 2017
ERR1630587.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1630587.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1383995
Sequences flagged as poor quality0
Sequence length43
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT42110.3042641050003793No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT39040.28208194393765873No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT30810.2226164111864566No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT21190.153107489550179No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGAACGA1409.458745E-1118.516
GGTATCA19000.018.51
ACGAACG1409.458745E-1118.515
CGAATTA2050.017.14634115
ACGATTA904.447012E-516.44444523
GTCTTAT1504.6729838E-916.0333331
TCCGATA1659.749783E-1015.696978
TAACGAA1659.749783E-1015.6969713
GTAACAC1353.973546E-715.0740733
TCTACAC1601.0968506E-815.031253
CGGGTCG750.004104968614.834
AACGATT1001.09362016E-414.79999922
GTATTGG1152.2103779E-514.4782611
GATAACG1803.3305696E-914.38888811
TAAGGTA908.27552E-414.3888884
ATAACGA1803.3305696E-914.38888812
GGACCGT908.27552E-414.3888886
CGATAAC1854.8985385E-914.010
CGTCGTA800.006299478513.87510
CCGATAA1753.5664925E-813.7428579
Produced by FastQC (version 0.11.5)