FastQCFastQC Report
Fri 10 Feb 2017
ERR1630586.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1630586.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1528501
Sequences flagged as poor quality0
Sequence length43
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT63060.412561064729431No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT55520.3632316890862355No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT53340.3489693497092904No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT31910.20876662821941236No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGGACC2050.022.5609768
CGGACCA2200.021.0227289
GACGGAC2300.020.1086967
GCGAAAG2400.019.27083218
AAGACGG2600.019.2115385
GGCTACG650.001580256317.0769231
TCAATAC1201.0418262E-716.9583323
CGAAAGC2650.016.75471719
ATGCGTG1352.2208042E-816.4444457
CGTTTAG904.4474997E-516.44444526
AGACGGA2950.016.3050846
CGCAAGA2850.016.228072
GGTATCA35900.015.9749311
GAGCGAA2800.015.85714216
GCGCAAG3350.015.4626871
CGGTGAT3750.015.29333214
CGCCTTA3300.014.57575725
TCGTTTA1405.998063E-714.53571430
TATTAGA2800.014.5357142
TAAACCG1152.2106768E-514.4782615
Produced by FastQC (version 0.11.5)