##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1630586.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1528501
Sequences flagged as poor quality	0
Sequence length	43
%GC	49
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.064955142325715	33.0	31.0	34.0	30.0	34.0
2	32.23247613184421	33.0	31.0	34.0	30.0	34.0
3	32.33769555924399	34.0	31.0	34.0	30.0	34.0
4	35.91318160733948	37.0	35.0	37.0	35.0	37.0
5	35.87097162514123	37.0	35.0	37.0	35.0	37.0
6	35.94647043083387	37.0	35.0	37.0	35.0	37.0
7	35.89584043451722	37.0	35.0	37.0	35.0	37.0
8	35.90018325143392	37.0	35.0	37.0	35.0	37.0
9	37.60525377477673	39.0	37.0	39.0	35.0	39.0
10	37.5203673402896	39.0	37.0	39.0	35.0	39.0
11	37.61063224688764	39.0	37.0	39.0	35.0	39.0
12	37.56341801542819	39.0	37.0	39.0	35.0	39.0
13	37.60131854673304	39.0	37.0	39.0	35.0	39.0
14	38.89339686398635	40.0	38.0	41.0	36.0	41.0
15	38.90157415664105	40.0	38.0	41.0	36.0	41.0
16	38.86361408988283	40.0	38.0	41.0	35.0	41.0
17	38.850735459119754	40.0	38.0	41.0	35.0	41.0
18	38.84044694769582	40.0	38.0	41.0	35.0	41.0
19	38.90336022024192	40.0	38.0	41.0	35.0	41.0
20	38.876530012083734	40.0	38.0	41.0	35.0	41.0
21	38.83212245199709	40.0	38.0	41.0	35.0	41.0
22	38.76226708389461	40.0	38.0	41.0	35.0	41.0
23	38.71567110522008	40.0	38.0	41.0	35.0	41.0
24	38.664450988255815	40.0	38.0	41.0	34.0	41.0
25	38.60879253595516	40.0	38.0	41.0	34.0	41.0
26	38.4753474155398	40.0	38.0	41.0	34.0	41.0
27	38.34632819998155	40.0	38.0	41.0	34.0	41.0
28	38.24971328118202	40.0	38.0	41.0	34.0	41.0
29	38.167052556720606	40.0	38.0	41.0	33.0	41.0
30	38.08319916048468	40.0	37.0	41.0	33.0	41.0
31	38.02479357226459	40.0	37.0	41.0	33.0	41.0
32	37.93409687007074	40.0	37.0	41.0	33.0	41.0
33	37.842891826698185	40.0	37.0	41.0	33.0	41.0
34	37.78295074716994	40.0	37.0	41.0	33.0	41.0
35	37.72015850823781	40.0	37.0	41.0	33.0	41.0
36	37.6779936683064	40.0	37.0	41.0	33.0	41.0
37	37.53314194756824	40.0	37.0	41.0	33.0	41.0
38	37.37998666667539	40.0	36.0	41.0	32.0	41.0
39	37.30552613311997	40.0	36.0	41.0	32.0	41.0
40	37.12188673739828	40.0	36.0	41.0	31.0	41.0
41	37.05263261195119	40.0	36.0	41.0	31.0	41.0
42	36.90603342752147	39.0	35.0	41.0	31.0	41.0
43	36.09901858094957	38.0	35.0	40.0	29.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	1.0
9	1.0
10	4.0
11	2.0
12	4.0
13	4.0
14	4.0
15	3.0
16	6.0
17	12.0
18	39.0
19	81.0
20	166.0
21	333.0
22	661.0
23	1166.0
24	1929.0
25	3242.0
26	5028.0
27	7387.0
28	10935.0
29	15570.0
30	21000.0
31	27536.0
32	35767.0
33	46270.0
34	61795.0
35	83435.0
36	118351.0
37	186631.0
38	346006.0
39	555132.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	44.572034954507714	18.844475731451926	11.615759492470074	24.967729821570284
2	19.302833298767876	20.914215954062183	33.59376277804202	26.189187969127925
3	21.339534615940718	23.43688358725313	28.46749854923222	26.75608324757393
4	14.658937089344398	14.965053997347727	34.22359553575693	36.15241337755094
5	14.541174654121914	36.8588571417356	33.977014081116074	14.622954123026416
6	35.55915239833013	33.96471444899284	15.34961377192426	15.126519380752775
7	29.303480992161603	29.964389948060223	21.367274211793124	19.364854847985054
8	28.46697516063123	31.669851704382268	19.89851495026827	19.964658184718232
9	27.200505593388556	13.372382484538772	19.877906524104336	39.549205397968336
10	17.701983839068472	26.42824571262956	32.12637741159476	23.743393036707204
11	35.71734660297899	21.685887022645062	21.102832121143525	21.493934253232418
12	22.95634742797028	25.35497196272688	28.2578814145362	23.43079919476664
13	30.949996107297277	19.657690770238293	24.814965773656674	24.577347348807756
14	23.41915379839464	20.57172353829013	25.29517481506391	30.713947848251326
15	26.408684063667607	26.985458301957276	22.599069284220292	24.006788350154824
16	25.37944037982311	25.826348821492427	24.2664545198204	24.527756278864064
17	23.947972556118707	25.820591546881555	25.48719300805168	24.74424288894806
18	23.710092436969294	24.409928420066457	27.21862792369779	24.661351219266457
19	24.660042747763985	24.789712273658964	27.17132667888343	23.37891829969362
20	25.251341019731093	24.10642845506807	26.650685868049806	23.991544657151024
21	25.437471090957743	24.435574461514907	26.30793175797726	23.81902268955009
22	25.35556077490299	24.500540071612644	26.42661012325147	23.717289030232887
23	24.654612591028727	24.5669450003631	26.2545461206764	24.52389628793177
24	24.35255194468306	25.159420896682438	26.432629092162845	24.05539806647166
25	24.60377847315769	24.99749754825152	26.156999570167113	24.24172440842368
26	24.45644458197934	25.256247787865366	26.243031571454644	24.044276058700646
27	24.509045136378713	24.692950806051154	26.30995988880609	24.488044168764038
28	23.98598365326552	24.9541871415197	26.630208289036123	24.429620916178663
29	23.84198636441847	25.428835179041425	27.02170296257575	23.707475493964346
30	24.020461877355658	25.516175651831436	26.433087057188708	24.030275413624196
31	23.894194377367107	26.001422308523185	26.28706163751283	23.817321676596876
32	23.547122311336402	25.34568181505933	26.443751099933856	24.663444773670413
33	23.296288324312513	25.632956733427065	26.81247836933047	24.258276572929947
34	24.499166176535052	24.767795375992556	26.420067765739113	24.31297068173328
35	23.743523883857453	25.219610585796147	26.710940980738645	24.325924549607752
36	23.366553243995263	25.969299333137496	26.441199580504037	24.222947842363205
37	23.924289221923964	24.831321667437575	26.97871967371955	24.265669436918916
38	23.68935316365511	25.1482334653363	26.839956270882386	24.3224571001262
39	23.589713058741864	25.026414768456156	27.037862585631284	24.3460095871707
40	23.083530858010562	24.840415544379756	27.50433267626256	24.571720921347122
41	22.403976183201713	25.090268177776785	27.791738441780538	24.714017197240956
42	22.945552538074885	24.94149496794572	27.926053041509295	24.1868994524701
43	22.912971597663333	24.528541361765548	27.672471264330216	24.886015776240907
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	67.0
1	97.0
2	127.0
3	478.0
4	829.0
5	829.0
6	1255.5
7	1682.0
8	1709.5
9	1737.0
10	2458.0
11	3179.0
12	3179.0
13	5568.0
14	7957.0
15	11174.5
16	14392.0
17	14002.0
18	13612.0
19	13612.0
20	15622.5
21	17633.0
22	16466.5
23	15300.0
24	17693.0
25	20086.0
26	20086.0
27	22788.5
28	25491.0
29	29719.5
30	33948.0
31	38861.0
32	43774.0
33	43774.0
34	49235.5
35	54697.0
36	60534.5
37	66372.0
38	71686.5
39	77001.0
40	77001.0
41	80416.5
42	83832.0
43	86270.0
44	88708.0
45	90722.0
46	92736.0
47	92736.0
48	96594.0
49	100452.0
50	100113.5
51	99775.0
52	99755.0
53	99735.0
54	99735.0
55	97041.5
56	94348.0
57	92616.5
58	90885.0
59	90771.0
60	90657.0
61	90657.0
62	83770.5
63	76884.0
64	70843.5
65	64803.0
66	56798.5
67	48794.0
68	48794.0
69	43468.5
70	38143.0
71	32273.5
72	26404.0
73	20552.5
74	14701.0
75	14701.0
76	11292.5
77	7884.0
78	6445.5
79	5007.0
80	4074.0
81	3141.0
82	3141.0
83	2457.5
84	1774.0
85	1370.5
86	967.0
87	786.5
88	606.0
89	606.0
90	452.0
91	298.0
92	175.0
93	52.0
94	38.5
95	25.0
96	25.0
97	14.5
98	4.0
99	3.0
100	2.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	1528501.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	62.897444735067054
#Duplication Level	Percentage of deduplicated	Percentage of total
1	85.95243732928958	54.06188676763306
2	9.224062752304206	11.603399543916886
3	2.1802012756324913	4.11387267736252
4	0.7903943831069283	1.9885514812150178
5	0.4216981439216631	1.3261867851096576
6	0.24801636464257878	0.9359757353099303
7	0.17115748253299265	0.7535757809028477
8	0.1227440809596089	0.6176231238970877
9	0.08967160266489758	0.5076103205628258
>10	0.6340407034135811	7.890035198678652
>50	0.0841542507078563	3.7261785415774953
>100	0.07652183134873873	9.158772593315096
>500	0.003961540000971912	1.5893159146171627
>1k	6.255063159429334E-4	0.5997146363185317
>5k	3.127531579714667E-4	1.1273008995832274
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	6306	0.412561064729431	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	5552	0.3632316890862355	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	5334	0.3489693497092904	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	3191	0.20876662821941236	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	3.9254145074160896E-4	0.0
2	0.0	0.0	0.0	0.0026169430049440595	0.0
3	0.0	6.54235751236015E-5	0.0	0.004906768134270112	0.0
4	0.0	6.54235751236015E-5	0.0	0.008439641190944593	0.0
5	0.0	6.54235751236015E-5	0.0	0.009093876942180607	0.0
6	0.0	6.54235751236015E-5	0.0	0.009093876942180607	0.0
7	0.0	6.54235751236015E-5	0.0	0.010402348444652636	0.0
8	0.0	6.54235751236015E-5	0.0	0.011318278496383057	0.0
9	0.0	6.54235751236015E-5	0.0	0.018122330309237614	0.0
10	0.0	6.54235751236015E-5	0.0	0.02499180569721577	0.0
11	6.54235751236015E-5	6.54235751236015E-5	0.0	0.028393831603643047	0.0
12	6.54235751236015E-5	6.54235751236015E-5	0.0	0.030421962432474692	0.0
13	6.54235751236015E-5	6.54235751236015E-5	0.0	0.03179585751007032	0.0
14	6.54235751236015E-5	6.54235751236015E-5	0.0	0.03428195336476718	0.0
15	6.54235751236015E-5	6.54235751236015E-5	0.0	0.037422284970700055	0.0
16	6.54235751236015E-5	6.54235751236015E-5	0.0	0.04232905310497016	0.0
17	6.54235751236015E-5	6.54235751236015E-5	0.0	0.047301244814363874	0.0
18	6.54235751236015E-5	6.54235751236015E-5	0.0	0.05024530569492595	0.0
19	6.54235751236015E-5	6.54235751236015E-5	0.0	0.05508665025407245	0.0
20	6.54235751236015E-5	6.54235751236015E-5	0.0	0.059404606212230156	0.0
21	6.54235751236015E-5	6.54235751236015E-5	0.0	0.06692831735144432	0.0
22	1.30847150247203E-4	6.54235751236015E-5	0.0	0.07726524222097336	0.0
23	1.30847150247203E-4	6.54235751236015E-5	0.0	0.09420994817798614	0.0
24	1.30847150247203E-4	6.54235751236015E-5	0.0	0.11514549221753863	0.0
25	1.30847150247203E-4	6.54235751236015E-5	0.0	0.12253835620650559	0.0
26	1.30847150247203E-4	6.54235751236015E-5	0.0	0.13529595335560787	0.0
27	1.30847150247203E-4	6.54235751236015E-5	0.0	0.14667965542711453	0.0
28	1.30847150247203E-4	6.54235751236015E-5	0.0	0.16826943521790302	0.0
29	1.30847150247203E-4	6.54235751236015E-5	0.0	0.1971212318474113	0.0
30	1.30847150247203E-4	6.54235751236015E-5	0.0	0.2467123017911012	0.0
31	1.30847150247203E-4	6.54235751236015E-5	0.0	0.41190682897819497	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
ACGGACC	205	0.0	22.560976	8
CGGACCA	220	0.0	21.022728	9
GACGGAC	230	0.0	20.108696	7
GCGAAAG	240	0.0	19.270832	18
AAGACGG	260	0.0	19.211538	5
GGCTACG	65	0.0015802563	17.076923	1
TCAATAC	120	1.0418262E-7	16.958332	3
CGAAAGC	265	0.0	16.754717	19
ATGCGTG	135	2.2208042E-8	16.444445	7
CGTTTAG	90	4.4474997E-5	16.444445	26
AGACGGA	295	0.0	16.305084	6
CGCAAGA	285	0.0	16.22807	2
GGTATCA	3590	0.0	15.974931	1
GAGCGAA	280	0.0	15.857142	16
GCGCAAG	335	0.0	15.462687	1
CGGTGAT	375	0.0	15.293332	14
CGCCTTA	330	0.0	14.575757	25
TCGTTTA	140	5.998063E-7	14.535714	30
TATTAGA	280	0.0	14.535714	2
TAAACCG	115	2.2106768E-5	14.478261	5
>>END_MODULE