FastQCFastQC Report
Fri 10 Feb 2017
ERR1630585.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1630585.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1873665
Sequences flagged as poor quality0
Sequence length43
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT82040.43785842186303314No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT73240.39089164818684236No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT59890.31964091766671204No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT43280.23099113235290195No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGACCG400.001931639523.1255
TAACGGC1950.018.9743636
TTAACGG2050.018.0487835
TCGCACG1502.5102054E-1017.26666622
ATACCGC2800.017.17857227
TACCGTC1757.2759576E-1216.9142867
GGTATCA40600.016.6317731
GACGGAC3350.016.5671637
CCGCTTA904.4483626E-516.44444525
ACGGACC3450.016.0869568
CGCAAGA3450.016.0869562
AAGACGG3500.015.8571435
CGGACCA3650.015.7123299
GCGAAAG3550.015.63380318
GCGCAAG4100.015.3414631
TCTAGCG2900.015.31034528
ATACCGT2201.8189894E-1215.1363646
CGAAAGC3700.015.019
ATCGCAC2109.094947E-1214.976191521
CGGTCCA3100.014.91935410
Produced by FastQC (version 0.11.5)