##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1630585.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1873665
Sequences flagged as poor quality	0
Sequence length	43
%GC	48
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.973183573370907	33.0	31.0	34.0	30.0	34.0
2	32.14355554488129	33.0	31.0	34.0	30.0	34.0
3	32.24510998497597	34.0	31.0	34.0	30.0	34.0
4	35.843130442208185	37.0	35.0	37.0	35.0	37.0
5	35.79917861517401	37.0	35.0	37.0	35.0	37.0
6	35.87725767413065	37.0	35.0	37.0	35.0	37.0
7	35.82559582422685	37.0	35.0	37.0	35.0	37.0
8	35.827559355594516	37.0	35.0	37.0	35.0	37.0
9	37.513900297011475	39.0	37.0	39.0	35.0	39.0
10	37.4143099219978	39.0	37.0	39.0	34.0	39.0
11	37.513325487747274	39.0	37.0	39.0	35.0	39.0
12	37.4623062287015	39.0	37.0	39.0	35.0	39.0
13	37.509963627436065	39.0	37.0	39.0	35.0	39.0
14	38.784179135544505	40.0	38.0	41.0	35.0	41.0
15	38.78564898207524	40.0	38.0	41.0	35.0	41.0
16	38.74396116701758	40.0	38.0	41.0	35.0	41.0
17	38.732113798357766	40.0	38.0	41.0	35.0	41.0
18	38.7195347087126	40.0	38.0	41.0	35.0	41.0
19	38.77959186941102	40.0	38.0	41.0	35.0	41.0
20	38.749241193062794	40.0	38.0	41.0	35.0	41.0
21	38.70602535672065	40.0	38.0	41.0	34.0	41.0
22	38.63706052042387	40.0	38.0	41.0	34.0	41.0
23	38.58159329442563	40.0	38.0	41.0	34.0	41.0
24	38.53319136558563	40.0	38.0	41.0	34.0	41.0
25	38.47485543039978	40.0	38.0	41.0	34.0	41.0
26	38.33794781884702	40.0	38.0	41.0	34.0	41.0
27	38.21053977098361	40.0	38.0	41.0	34.0	41.0
28	38.11028972628512	40.0	38.0	41.0	33.0	41.0
29	38.036239669311215	40.0	37.0	41.0	33.0	41.0
30	37.943494167847504	40.0	37.0	41.0	33.0	41.0
31	37.89758147801234	40.0	37.0	41.0	33.0	41.0
32	37.799976516613164	40.0	37.0	41.0	33.0	41.0
33	37.71801255827482	40.0	37.0	41.0	33.0	41.0
34	37.64850973893412	40.0	37.0	41.0	33.0	41.0
35	37.58578721382958	40.0	37.0	41.0	33.0	41.0
36	37.54528637723392	40.0	37.0	41.0	33.0	41.0
37	37.40713841588544	40.0	36.0	41.0	32.0	41.0
38	37.261262285413885	40.0	36.0	41.0	31.0	41.0
39	37.19165325711907	40.0	36.0	41.0	31.0	41.0
40	37.00184131101344	39.0	36.0	41.0	31.0	41.0
41	36.94309068056456	39.0	36.0	41.0	31.0	41.0
42	36.79217256019619	39.0	35.0	41.0	31.0	41.0
43	36.00278171391364	38.0	35.0	40.0	29.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	2.0
9	4.0
10	7.0
11	3.0
12	4.0
13	2.0
14	4.0
15	4.0
16	9.0
17	24.0
18	33.0
19	113.0
20	251.0
21	489.0
22	861.0
23	1585.0
24	2667.0
25	4291.0
26	6740.0
27	9995.0
28	14441.0
29	20492.0
30	27413.0
31	35872.0
32	46329.0
33	59479.0
34	79223.0
35	106431.0
36	149887.0
37	235312.0
38	445325.0
39	626373.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	43.9965522118415	19.412434987044108	12.099121240990252	24.49189156012414
2	19.16521896923943	20.895837836539616	34.14799337128035	25.7909498229406
3	20.847590150854074	23.60176445629288	29.000328233702394	26.550317159150648
4	14.466940461608665	15.27012566280525	34.57469718439529	35.688236691190795
5	14.295778594359184	37.2418762158657	34.25969957276248	14.202645617012646
6	35.158526203990576	34.47857541236027	15.480355346339927	14.882543037309231
7	28.990187680295037	30.46393031838669	21.66977554685603	18.876106454462242
8	27.934716184590098	32.364536883594454	20.011901807420216	19.688845124395236
9	26.75574342265026	13.47812976172368	20.42600998577654	39.34011682984952
10	17.544972020078294	26.66367787197818	32.78862550135697	23.002724606586554
11	35.286350548257026	21.92739897473668	21.50651263699754	21.279737840008753
12	22.267801341221617	25.916692685191855	28.64220658442144	23.173299389165088
13	30.138952267347683	20.022789559499696	25.45753910117337	24.380719071979247
14	22.865987249588375	21.11273893678966	25.750974693982116	30.270299119639848
15	25.837062655277226	27.772093730736287	23.03629517549829	23.354548438488205
16	24.972447048965527	26.29109259125831	24.686803670880334	24.049656688895826
17	23.47810307605682	26.471647813243028	25.869192198178435	24.181056912521715
18	23.105624538004392	25.002548481185272	27.76286049000222	24.12896649080812
19	24.008454019261713	25.288512087272807	27.724273015720524	22.978760877744957
20	24.49536069681613	24.724537203822454	27.32612286614736	23.45397923321405
21	24.70446958234263	25.05858838159436	26.955565696108962	23.281376339954047
22	24.701160559651804	25.074866638379863	27.071968574958703	23.15200422700963
23	24.044372926857253	25.12562277675038	26.88468856492489	23.94531573146747
24	23.67173427480366	25.76191581739532	26.93629864463498	23.63005126316604
25	24.007333221253532	25.51341888758129	26.773089106110216	23.70615878505496
26	23.717313393803057	25.968836478239172	26.887623988279657	23.426226139678118
27	24.077943495768988	25.17461766110804	26.82688741050294	23.920551432620023
28	23.372000864615604	25.53812981509501	27.373676724494505	23.716192595794872
29	23.468602978654136	25.9051644771077	27.47652328457862	23.149709259659545
30	23.400447785489938	26.081076393058524	27.089581115087274	23.428894706364265
31	23.288688212674092	26.36810742582052	26.916231023155152	23.426973338350237
32	22.890111092431145	25.795379643639606	26.941315550004933	24.373193713924316
33	22.555472829988286	26.014362225904847	27.432652048258362	23.997512895848512
34	23.96869237563812	25.185879012523582	27.22727915609247	23.618149455745822
35	23.304539498789804	25.526014522339906	27.41109002943429	23.758355949435998
36	22.789933099033178	26.123880202704324	27.187143913132815	23.89904278512968
37	23.32786277162673	25.05538610157099	27.698548032866064	23.918203093936217
38	23.036989002836687	25.141580805533543	27.71087681095607	24.110553380673707
39	22.966965812992186	25.006818187883106	28.069638916241697	23.956577082883012
40	22.414465766292267	24.96700317292579	28.435712894247374	24.182818166534574
41	21.7834564876859	25.160847857007525	28.8093656016417	24.246330053664877
42	22.135493804922437	25.19649990793445	28.928276933176424	23.73972935396669
43	22.200873688733044	24.586091964145137	28.668732137281744	24.544302209840072
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	324.0
1	314.5
2	305.0
3	850.0
4	1395.0
5	1395.0
6	1994.5
7	2594.0
8	2511.5
9	2429.0
10	3507.5
11	4586.0
12	4586.0
13	7968.5
14	11351.0
15	15112.0
16	18873.0
17	18352.0
18	17831.0
19	17831.0
20	20733.5
21	23636.0
22	22263.5
23	20891.0
24	23859.5
25	26828.0
26	26828.0
27	30929.5
28	35031.0
29	40052.5
30	45074.0
31	51385.0
32	57696.0
33	57696.0
34	65164.5
35	72633.0
36	79635.0
37	86637.0
38	93883.5
39	101130.0
40	101130.0
41	104895.0
42	108660.0
43	111292.0
44	113924.0
45	114882.0
46	115840.0
47	115840.0
48	119965.0
49	124090.0
50	123036.0
51	121982.0
52	120794.0
53	119606.0
54	119606.0
55	117271.5
56	114937.0
57	111128.5
58	107320.0
59	105841.5
60	104363.0
61	104363.0
62	96512.0
63	88661.0
64	80226.0
65	71791.0
66	62447.0
67	53103.0
68	53103.0
69	46620.0
70	40137.0
71	33908.5
72	27680.0
73	20995.0
74	14310.0
75	14310.0
76	10762.5
77	7215.0
78	5742.0
79	4269.0
80	3502.0
81	2735.0
82	2735.0
83	2103.0
84	1471.0
85	1273.5
86	1076.0
87	933.5
88	791.0
89	791.0
90	591.5
91	392.0
92	222.5
93	53.0
94	30.5
95	8.0
96	8.0
97	7.0
98	6.0
99	3.5
100	1.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	1873665.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	59.9197574780075
#Duplication Level	Percentage of deduplicated	Percentage of total
1	86.65415865912183	51.92296171315364
2	8.450125785416024	10.126589754415715
3	2.0206399303131417	3.6322876372422424
4	0.8150012395170202	1.953387064645414
5	0.43396683849793355	1.300159385814691
6	0.27006003300741155	0.9709159009384081
7	0.17900234809544138	0.7508044100110908
8	0.14118618138285385	0.6767873398165264
9	0.10990427088342267	0.5926893531418734
>10	0.729010286751248	8.68028174690687
>50	0.09902333812331367	4.1734529820141555
>100	0.09006671171192474	10.57507597357739
>500	0.006426308600470054	2.4761145864815077
>1k	0.0011603057195293154	1.0177425123813275
>5k	2.6776285835291896E-4	1.1507496394592236
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	8204	0.43785842186303314	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	7324	0.39089164818684236	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	5989	0.31964091766671204	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	4328	0.23099113235290195	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	1.0674266744588814E-4	0.0
2	2.1348533489177628E-4	5.337133372294407E-5	0.0	9.606840070129932E-4	0.0
3	2.1348533489177628E-4	5.337133372294407E-5	0.0	0.001601140011688322	0.0
4	2.1348533489177628E-4	5.337133372294407E-5	0.0	0.0034157653582684204	0.0
5	3.2022800233766444E-4	1.0674266744588814E-4	0.0	0.003629250693160197	0.0
6	3.2022800233766444E-4	1.0674266744588814E-4	0.0	0.0036826220268831407	0.0
7	4.2697066978355255E-4	1.0674266744588814E-4	0.0	0.0041095926966666935	0.0
8	4.2697066978355255E-4	1.0674266744588814E-4	0.0	0.004376449365281414	0.0
9	4.2697066978355255E-4	1.0674266744588814E-4	0.0	0.007578729388658058	0.0
10	4.2697066978355255E-4	1.0674266744588814E-4	0.0	0.01088775207948059	0.0
11	4.2697066978355255E-4	1.6011400116883222E-4	0.0	0.012488892091168912	0.0
12	4.2697066978355255E-4	1.6011400116883222E-4	0.0	0.013289462097013073	0.0
13	4.803420035064966E-4	1.6011400116883222E-4	0.0	0.014090032102857234	0.0
14	4.803420035064966E-4	1.6011400116883222E-4	0.0	0.014997344776147283	0.0
15	6.938273383982729E-4	1.6011400116883222E-4	0.0	0.01702545545761916	0.0
16	6.938273383982729E-4	2.6685666861472033E-4	0.0	0.020014250146104026	0.0
17	8.00570005844161E-4	2.6685666861472033E-4	0.0	0.02449744217883133	0.0
18	8.00570005844161E-4	2.6685666861472033E-4	0.0	0.026792409528917923	0.0
19	8.539413395671051E-4	2.6685666861472033E-4	0.0	0.029941318218571624	0.0
20	8.539413395671051E-4	2.6685666861472033E-4	0.0	0.032609884904718826	0.0
21	8.539413395671051E-4	2.6685666861472033E-4	0.0	0.03709307693744613	0.0
22	8.539413395671051E-4	2.6685666861472033E-4	0.0	0.044404949657489465	0.0
23	8.539413395671051E-4	3.735993360606085E-4	0.0	0.0562000144102601	0.0
24	8.539413395671051E-4	4.2697066978355255E-4	0.0	0.07349232653649398	0.0
25	8.539413395671051E-4	4.2697066978355255E-4	0.0	0.07925643057857194	0.0
26	9.073126732900492E-4	4.2697066978355255E-4	0.0	0.08945035531965426	0.0
27	0.0010674266744588813	4.2697066978355255E-4	0.0	0.10364713008995738	0.0
28	0.0013876546767965458	4.803420035064966E-4	0.0	0.1363637576621221	0.0
29	0.0014410260105194898	5.337133372294407E-4	0.0	0.18434458667904882	0.0
30	0.0014410260105194898	5.337133372294407E-4	0.0	0.25538183186428737	0.0
31	0.0014410260105194898	5.337133372294407E-4	0.0	0.5033984196748085	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TAGACCG	40	0.0019316395	23.125	5
TAACGGC	195	0.0	18.97436	36
TTAACGG	205	0.0	18.04878	35
TCGCACG	150	2.5102054E-10	17.266666	22
ATACCGC	280	0.0	17.178572	27
TACCGTC	175	7.2759576E-12	16.914286	7
GGTATCA	4060	0.0	16.631773	1
GACGGAC	335	0.0	16.567163	7
CCGCTTA	90	4.4483626E-5	16.444445	25
ACGGACC	345	0.0	16.086956	8
CGCAAGA	345	0.0	16.086956	2
AAGACGG	350	0.0	15.857143	5
CGGACCA	365	0.0	15.712329	9
GCGAAAG	355	0.0	15.633803	18
GCGCAAG	410	0.0	15.341463	1
TCTAGCG	290	0.0	15.310345	28
ATACCGT	220	1.8189894E-12	15.136364	6
CGAAAGC	370	0.0	15.0	19
ATCGCAC	210	9.094947E-12	14.9761915	21
CGGTCCA	310	0.0	14.919354	10
>>END_MODULE