FastQCFastQC Report
Fri 10 Feb 2017
ERR1630584.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1630584.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1922638
Sequences flagged as poor quality0
Sequence length43
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT98760.5136692398673073No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT93330.48542679381141957No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT79680.4144305896377789No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT56370.2931909178951004No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTAGCGG1551.8189894E-1219.09677329
CGTATGC953.6089423E-617.52631631
TCTAGCG1705.456968E-1217.41176428
CTAGTAC700.002593343815.8571433
TACGTTA1302.591405E-715.65384619
CTTAACG1455.351103E-815.31034530
ACGTTAA1353.9758925E-715.07407320
CGCTAAG1353.9758925E-715.07407335
ATCTCGC3850.014.89610411
CGCAATA2006.184564E-1114.836
CGGCCTT4000.014.33750124
TAGGGGT1956.730261E-1014.230774
ATACGCT1304.447418E-614.2307698
TCTTATA18750.014.20837
GACGGTA2101.364242E-1014.0952398
TCGCCAT4350.014.03448313
AACGCTA1601.7851016E-713.87500133
GGTATCA58250.013.7836911
ATAGGGG1907.1322575E-913.6315793
GAACACG950.00124560713.6315796
Produced by FastQC (version 0.11.5)