FastQCFastQC Report
Fri 10 Feb 2017
ERR1630583.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1630583.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences875974
Sequences flagged as poor quality0
Sequence length43
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT48640.5552676220983728No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT41930.4786671750531409No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT33850.3864269944085098No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT25990.29669830383093565No Hit
ACCTAGGCCTGGTCAGCATGTTGATGTATCTACGGAGATCAGC14520.1657583444257478No Hit
GGCCTAGGTATGGGAAAAGACACAAAGAGGACACGCTGGCCTT12470.14235582334635502No Hit
GTGTGGGAGCAGGGAGCAAGCTTTGGCCAGAGCCAAGGGTGCA10850.12386212376166415No Hit
GCTTTATTGAGCCTGTGTGGGAGCAGGGAGCAAGCTTTGGCCA10330.11792587451225722No Hit
GTATGCAGCTGATCTCCGTAGATACATCAACATGCTGACCAGG9110.10399852050403322No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACCGTCA502.701391E-422.28
GCCGTCT1403.6379788E-1219.82142836
GATCTAT500.00703357818.51
GGTATCA21100.016.5710891
ATGCCGT1801.0913936E-1116.44444534
GTTATCT904.444013E-516.4444453
CAAGACG2050.016.2439024
TGCCGTC1751.3096724E-1015.85714235
CGGACCA1751.3096724E-1015.8571429
CCGTCTT2003.6379788E-1215.72537
GACGGAC1802.0190782E-1015.4166677
CTTATCC1455.3396434E-815.3103453
CACCGTC855.3623406E-415.2352947
GACCGCG1601.0948497E-815.031257
GCTTTAT4950.014.9494951
GACACGC5600.014.86607130
CGTATTG750.0041033714.79999927
TAACCCG1508.092138E-814.79999928
ACCCGTT1508.092138E-814.79999930
GTGCGCC750.0041033714.79999911