FastQCFastQC Report
Fri 10 Feb 2017
ERR1630582.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1630582.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2071325
Sequences flagged as poor quality0
Sequence length43
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT57470.27745525207294847No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT57060.27547584275765513No Hit
GCTTAGGACACAGAGCACATCAAAAGTTCCCAAAGAGGGCTTG54180.26157169927461893No Hit
CTGTAGAACAGAGCAGGTGAAGAGAGAGCAAGCCCTCTTTGGG47450.2290804195382183No Hit
GTTCTACAGCACACTACCAGAAGACAGCAGAAATGAAAAGCAT47120.22748723643078705No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT44720.21590045019492357No Hit
TCCCAGAAGAGGTCGCCATTGTTGAAGAACTTGGCCGCAGACA41140.19861682739309378No Hit
CTTCTGGGAAATCTCGCCTTCCTCGGCCTTTCACCAGCCAAGC39760.19195442530747225No Hit
GGTGAAGAGAGAGCAAGCCCTCTTTGGGAACTTTTGATGTGCT33050.1595597021230372No Hit
GAATACACCTCTTAAATTTACAGGACTTAACATTTCAAACATC31880.1539111438330537No Hit
CGCCTGGAGTCCAGATACTTGCTGTAGTCACTGGTGAATGTGC30100.1453176107081216No Hit
CTCCAGGCGTGCCCAAGATTTTGTGCAGTGGTTGATGAATACC29050.14024839172993134No Hit
ATTGAAAGCTGAGTATTTTTAAGACAAAGGTTTCAGGAAGAAA28960.13981388724608643No Hit
CTTCAAGACACAGAGGAGAAATCCAGATCATTCTCAGCTTCCC28540.13778619965481034No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT27620.13334459826439599No Hit
GTGATATAGTTATTTCCTGTCAGTGATTTTGGTCTGAATCAAC27380.1321859196408096No Hit
GTTTATAAAGTCCCTGGCGGCAAGATTATCAAGAATGGTGTTC27070.13068929308534394No Hit
CTACCAGAAGACAGCAGAAATGAAAAGCATTTACTTTGTGGCT26980.13025478860149903No Hit
CTCTTCACCTGCTCTGTTCTACAGCACACTACCAGAAGACAGC26770.12924094480586099No Hit
CAGTGATCCTGATCAGATGAACGAGGACAAGCGCCATTCACAG26300.12697186583467104No Hit
GGAATAACATTGCCAAACGTCACGATGAATTTGAGAGACATGC25190.12161297720058417No Hit
GTGTTCATCTCATCAGAGAAAGAACCATCAGCATGTCTGCGGC24830.11987495926520464No Hit
GATGAACACCATTCTTGATAATCTTGCCGCCAGGGACTTTATA24820.11982668098922188No Hit
ATTCAAGATCATCTTCACAACATCACCTGCTAGCCACGTGGGA24740.11944045478135974No Hit
CTATATCACTATTCAAGATCATCTTCACAACATCACCTGCTAG24520.11837833270973894No Hit
GGTGTATTCTGAGGCCACATTGCTTTGCATGCCAATAAATAAA24090.11630236684248005No Hit
GTACAAGGCAGCTGGCAACGTTCCCTTCAAGACACAGAGGAGA23740.1146126271830833No Hit
ATCTTGAATAGTGATATAGTTATTTCCTGTCAGTGATTTTGGT23710.11446779235513499No Hit
TATCTGGACTCCAGGCGTGCCCAAGATTTTGTGCAGTGGTTGA23560.11374361821539353No Hit
CTTTATAAACTGGTTGATTCAGACCAAAATCACTGACAGGAAA23330.11263321786778993No Hit
GGATCACTGAGTGGGTCTGCCTGGGAAGCTGAGAATGATCTGG23320.11258493959180717No Hit
GCCTTGTACCAGCATTACAAATAATCCAGCCACAAAGTAAATG22280.10756399888959965No Hit
ATCCTGATCAGATGAACGAGGACAAGCGCCATTCACAGGGCAC20820.10051537059611601No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATACGG454.0103005E-628.7777772
CGAATTA1355.638867E-1119.18518615
GTATACG500.00703674218.4999981
GTATAGA1850.018.01
GTAAGAC1504.6766218E-916.0333333
CTACACG700.002593434615.8571434
GGTATCA30650.015.7536711
CTTATAC10300.015.62621337
GCTTAGG14500.015.0551731
ATCTCGC10950.014.8675811
TCTTATA16200.014.84567937
TAGGACA15350.014.8241044
CGAACTA750.00410588314.829
TCTATAC1252.9613348E-614.7999993
CGGTGAT2400.014.64583414
TTAGGAC15950.014.6144193
TAATACT2800.014.5357154
GGTATAT1152.211426E-514.4782611
GGTCTAC1152.211426E-514.4782611
CGGCCTT11250.014.47111124
Produced by FastQC (version 0.11.5)