##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1630582.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	2071325
Sequences flagged as poor quality	0
Sequence length	43
%GC	47
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.06428349003657	33.0	31.0	34.0	30.0	34.0
2	32.249544132379036	34.0	31.0	34.0	30.0	34.0
3	32.34346855273798	34.0	31.0	34.0	30.0	34.0
4	35.92626700300532	37.0	35.0	37.0	35.0	37.0
5	35.883145812463034	37.0	35.0	37.0	35.0	37.0
6	35.95902381325963	37.0	35.0	37.0	35.0	37.0
7	35.911670066261934	37.0	35.0	37.0	35.0	37.0
8	35.90672830193234	37.0	35.0	37.0	35.0	37.0
9	37.6287381265615	39.0	37.0	39.0	35.0	39.0
10	37.54213124449326	39.0	37.0	39.0	35.0	39.0
11	37.63232037463942	39.0	37.0	39.0	35.0	39.0
12	37.581863300061556	39.0	37.0	39.0	35.0	39.0
13	37.61053818208152	39.0	37.0	39.0	35.0	39.0
14	38.932485727734665	40.0	38.0	41.0	36.0	41.0
15	38.95601173162106	40.0	38.0	41.0	36.0	41.0
16	38.914797532980096	40.0	38.0	41.0	36.0	41.0
17	38.893798896841396	40.0	38.0	41.0	35.0	41.0
18	38.861270442832485	40.0	38.0	41.0	35.0	41.0
19	38.9411874041978	40.0	38.0	41.0	35.0	41.0
20	38.90995232520247	40.0	38.0	41.0	35.0	41.0
21	38.862930249960776	40.0	38.0	41.0	35.0	41.0
22	38.815968039781296	40.0	38.0	41.0	35.0	41.0
23	38.76928149855769	40.0	38.0	41.0	35.0	41.0
24	38.72979179993482	40.0	38.0	41.0	35.0	41.0
25	38.680369811594026	40.0	38.0	41.0	34.0	41.0
26	38.554601523179606	40.0	38.0	41.0	34.0	41.0
27	38.43345781082157	40.0	38.0	41.0	34.0	41.0
28	38.35656548344659	40.0	38.0	41.0	34.0	41.0
29	38.30258168080818	40.0	38.0	41.0	34.0	41.0
30	38.23143639940618	40.0	38.0	41.0	34.0	41.0
31	38.18753889418609	40.0	38.0	41.0	34.0	41.0
32	38.100019552701774	40.0	38.0	41.0	33.0	41.0
33	38.03553039720951	40.0	38.0	41.0	33.0	41.0
34	37.98910600702449	40.0	37.0	41.0	33.0	41.0
35	37.950389243600114	40.0	37.0	41.0	33.0	41.0
36	37.91687591276116	40.0	37.0	41.0	33.0	41.0
37	37.806795650127334	40.0	37.0	41.0	33.0	41.0
38	37.6612245784703	40.0	37.0	41.0	33.0	41.0
39	37.621045948849165	40.0	37.0	41.0	33.0	41.0
40	37.48195913243938	40.0	37.0	41.0	32.0	41.0
41	37.43572544144449	40.0	37.0	41.0	32.0	41.0
42	37.33452451932941	40.0	37.0	41.0	32.0	41.0
43	36.54569225012975	39.0	35.0	40.0	30.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	1.0
8	1.0
9	4.0
10	7.0
11	8.0
12	5.0
13	4.0
14	0.0
15	5.0
16	5.0
17	16.0
18	43.0
19	107.0
20	195.0
21	387.0
22	768.0
23	1279.0
24	2306.0
25	3733.0
26	6038.0
27	9056.0
28	13166.0
29	18956.0
30	26222.0
31	34837.0
32	45929.0
33	59306.0
34	79979.0
35	108140.0
36	154608.0
37	242273.0
38	466423.0
39	797518.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	43.34515346456978	19.05389062556576	12.420503783809881	25.180452126054583
2	18.118836976331576	21.429471473573678	35.97199859993	24.47969295016475
3	20.084969765729667	23.603056014869708	30.540692551868975	25.771281667531653
4	14.781697705574933	15.56641280339879	34.0473368496011	35.60455264142517
5	13.8775904312457	37.83211229526994	34.529009209081146	13.761288064403221
6	34.849770074710634	34.43032841297237	15.970743364754444	14.74915814756255
7	28.270020397571606	31.129590962306736	21.53273870590081	19.06764993422085
8	25.972167573895938	35.03535176758838	20.328871615994593	18.66360904252109
9	26.02199075471015	14.534223262887283	19.644575332214885	39.79921065018768
10	16.22584577504737	28.072513970526114	33.4269127249466	22.274727529479925
11	34.766924553124205	22.13288595464256	22.2358635173138	20.864325974919435
12	20.933460466126753	26.167211808866302	29.068349969222602	23.830977755784343
13	29.25344887934052	20.2462192075121	26.18227463097293	24.318057282174454
14	22.506704645577106	21.395049062797966	25.149505751149626	30.9487405404753
15	25.16949295740649	28.508225411270566	23.158075145136568	23.16420648618638
16	25.10991756484376	27.153874935126066	23.908609223564625	23.827598276465547
17	23.79254824810206	27.230347724282765	25.31775554294956	23.659348484665614
18	22.2593750377174	25.898977707506067	27.50543734083256	24.336209913943975
19	24.143289923116846	26.24151207560378	27.309620653446466	22.30557734783291
20	24.33234765186535	25.093502951009622	27.724186209310464	22.849963187814563
21	23.60604926798064	26.569273291250767	26.017549153319735	23.807128287448855
22	23.880897493150517	25.648992794467308	26.147079767781495	24.323029944600677
23	22.92798088180271	26.6627400335534	26.524712442518677	23.88456664212521
24	24.875526534947436	26.296018249188318	26.199847923430653	22.628607292433585
25	24.316174429411124	25.508551289633445	26.579894511966977	23.59537976898845
26	24.2529298878737	25.85982402568404	26.25020216528068	23.637043921161574
27	24.10780539026951	25.56151255838654	26.073214005527863	24.257468045816083
28	23.060601305927364	26.020011345394856	26.974183191918215	23.945204156759562
29	23.260135420564133	26.829251807417958	26.464605988920137	23.446006783097776
30	22.765427926568744	27.67243189745694	26.365201018671623	23.196939157302694
31	23.780575235658336	26.255947280122626	26.658539823542903	23.304937660676135
32	22.834610696052042	26.411499885339097	26.689534476723836	24.064354941885025
33	23.1285771185111	25.934751910009297	26.79912616320471	24.137544808274896
34	23.357126477013505	26.099477387662485	26.606447563757495	23.93694857156651
35	23.71554439790955	26.092332202817037	26.50467695798583	23.687446441287584
36	23.237444630852234	26.534174984611298	26.659795058718455	23.568585325818013
37	24.359093816759803	25.021616598071283	26.44148069472439	24.17780889044452
38	23.22619579254825	25.713444292904303	27.707868152028293	23.352491762519158
39	23.321835057270103	25.48610189130146	27.522141624322593	23.669921427105837
40	22.955258107732973	24.708628534875018	28.513970526112516	23.822142831279493
41	22.160694241608635	25.788613568609463	28.435663162468465	23.615029027313433
42	23.258156011248836	24.59700916080287	28.030077365937263	24.11475746201103
43	22.07060697862479	25.144581366999386	28.192582042895246	24.592229611480573
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	96.0
1	123.0
2	150.0
3	495.0
4	840.0
5	840.0
6	1219.5
7	1599.0
8	1662.0
9	1725.0
10	2584.5
11	3444.0
12	3444.0
13	5584.0
14	7724.0
15	11981.0
16	16238.0
17	16173.5
18	16109.0
19	16109.0
20	17988.5
21	19868.0
22	21174.5
23	22481.0
24	24820.5
25	27160.0
26	27160.0
27	30514.5
28	33869.0
29	46970.5
30	60072.0
31	61700.5
32	63329.0
33	63329.0
34	69454.0
35	75579.0
36	80207.5
37	84836.0
38	103401.0
39	121966.0
40	121966.0
41	131929.5
42	141893.0
43	131479.5
44	121066.0
45	130288.5
46	139511.0
47	139511.0
48	148165.5
49	156820.0
50	163007.0
51	169194.0
52	179329.0
53	189464.0
54	189464.0
55	159113.0
56	128762.0
57	119832.5
58	110903.0
59	99246.5
60	87590.0
61	87590.0
62	81427.5
63	75265.0
64	67358.5
65	59452.0
66	51878.5
67	44305.0
68	44305.0
69	38274.0
70	32243.0
71	27707.5
72	23172.0
73	18769.5
74	14367.0
75	14367.0
76	11404.0
77	8441.0
78	6901.5
79	5362.0
80	4176.5
81	2991.0
82	2991.0
83	2224.0
84	1457.0
85	1216.0
86	975.0
87	807.5
88	640.0
89	640.0
90	482.0
91	324.0
92	177.5
93	31.0
94	19.5
95	8.0
96	8.0
97	5.5
98	3.0
99	2.0
100	1.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	2071325.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	47.1218649797789
#Duplication Level	Percentage of deduplicated	Percentage of total
1	84.3942196967322	39.76813025623211
2	9.303944720153305	8.768384537647817
3	2.5565390123452127	3.6140665846580524
4	1.057513229705133	1.9932798249798076
5	0.5923460630687005	1.3956225602613452
6	0.3869539359934847	1.0940394675167395
7	0.248056260780546	0.81822115395228
8	0.18916430336816228	0.7131019809846783
9	0.14776673375332816	0.6266739668784539
>10	0.8512282102928213	7.755239375889371
>50	0.11096817258506306	3.6518572050123925
>100	0.12713214934018724	12.57626087738363
>500	0.02400960294400478	7.838462140556232
>1k	0.00985009351548914	8.570956397496584
>5k	3.078154223590356E-4	0.8157036705505405
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	5747	0.27745525207294847	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	5706	0.27547584275765513	No Hit
GCTTAGGACACAGAGCACATCAAAAGTTCCCAAAGAGGGCTTG	5418	0.26157169927461893	No Hit
CTGTAGAACAGAGCAGGTGAAGAGAGAGCAAGCCCTCTTTGGG	4745	0.2290804195382183	No Hit
GTTCTACAGCACACTACCAGAAGACAGCAGAAATGAAAAGCAT	4712	0.22748723643078705	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	4472	0.21590045019492357	No Hit
TCCCAGAAGAGGTCGCCATTGTTGAAGAACTTGGCCGCAGACA	4114	0.19861682739309378	No Hit
CTTCTGGGAAATCTCGCCTTCCTCGGCCTTTCACCAGCCAAGC	3976	0.19195442530747225	No Hit
GGTGAAGAGAGAGCAAGCCCTCTTTGGGAACTTTTGATGTGCT	3305	0.1595597021230372	No Hit
GAATACACCTCTTAAATTTACAGGACTTAACATTTCAAACATC	3188	0.1539111438330537	No Hit
CGCCTGGAGTCCAGATACTTGCTGTAGTCACTGGTGAATGTGC	3010	0.1453176107081216	No Hit
CTCCAGGCGTGCCCAAGATTTTGTGCAGTGGTTGATGAATACC	2905	0.14024839172993134	No Hit
ATTGAAAGCTGAGTATTTTTAAGACAAAGGTTTCAGGAAGAAA	2896	0.13981388724608643	No Hit
CTTCAAGACACAGAGGAGAAATCCAGATCATTCTCAGCTTCCC	2854	0.13778619965481034	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	2762	0.13334459826439599	No Hit
GTGATATAGTTATTTCCTGTCAGTGATTTTGGTCTGAATCAAC	2738	0.1321859196408096	No Hit
GTTTATAAAGTCCCTGGCGGCAAGATTATCAAGAATGGTGTTC	2707	0.13068929308534394	No Hit
CTACCAGAAGACAGCAGAAATGAAAAGCATTTACTTTGTGGCT	2698	0.13025478860149903	No Hit
CTCTTCACCTGCTCTGTTCTACAGCACACTACCAGAAGACAGC	2677	0.12924094480586099	No Hit
CAGTGATCCTGATCAGATGAACGAGGACAAGCGCCATTCACAG	2630	0.12697186583467104	No Hit
GGAATAACATTGCCAAACGTCACGATGAATTTGAGAGACATGC	2519	0.12161297720058417	No Hit
GTGTTCATCTCATCAGAGAAAGAACCATCAGCATGTCTGCGGC	2483	0.11987495926520464	No Hit
GATGAACACCATTCTTGATAATCTTGCCGCCAGGGACTTTATA	2482	0.11982668098922188	No Hit
ATTCAAGATCATCTTCACAACATCACCTGCTAGCCACGTGGGA	2474	0.11944045478135974	No Hit
CTATATCACTATTCAAGATCATCTTCACAACATCACCTGCTAG	2452	0.11837833270973894	No Hit
GGTGTATTCTGAGGCCACATTGCTTTGCATGCCAATAAATAAA	2409	0.11630236684248005	No Hit
GTACAAGGCAGCTGGCAACGTTCCCTTCAAGACACAGAGGAGA	2374	0.1146126271830833	No Hit
ATCTTGAATAGTGATATAGTTATTTCCTGTCAGTGATTTTGGT	2371	0.11446779235513499	No Hit
TATCTGGACTCCAGGCGTGCCCAAGATTTTGTGCAGTGGTTGA	2356	0.11374361821539353	No Hit
CTTTATAAACTGGTTGATTCAGACCAAAATCACTGACAGGAAA	2333	0.11263321786778993	No Hit
GGATCACTGAGTGGGTCTGCCTGGGAAGCTGAGAATGATCTGG	2332	0.11258493959180717	No Hit
GCCTTGTACCAGCATTACAAATAATCCAGCCACAAAGTAAATG	2228	0.10756399888959965	No Hit
ATCCTGATCAGATGAACGAGGACAAGCGCCATTCACAGGGCAC	2082	0.10051537059611601	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0016897396593967629	0.0
3	0.0	0.0	0.0	0.00280014000700035	0.0
4	0.0	0.0	0.0	0.0046829927703281714	0.0
5	0.0	0.0	0.0	0.005407166910069642	0.0
6	0.0	0.0	0.0	0.005503723462035171	0.0
7	0.0	0.0	0.0	0.006131341049811111	0.0
8	0.0	0.0	0.0	0.006372732429724934	0.0
9	0.0	0.0	0.0	0.011586786235863518	0.0
10	0.0	4.827827598276466E-5	0.0	0.0168008400420021	0.0
11	0.0	1.4483482794829398E-4	0.0	0.018635414529347156	0.0
12	0.0	1.4483482794829398E-4	0.0	0.019649258324985215	0.0
13	0.0	1.4483482794829398E-4	0.0	0.0207596586725888	0.0
14	0.0	1.4483482794829398E-4	0.0	0.02177350246822686	0.0
15	0.0	1.4483482794829398E-4	0.0	0.023897746611468505	0.0
16	0.0	1.4483482794829398E-4	0.0	0.026311660410606737	0.0
17	0.0	1.4483482794829398E-4	0.0	0.029208356969572618	0.0
18	0.0	1.4483482794829398E-4	0.0	0.031042931456917673	0.0
19	0.0	1.4483482794829398E-4	0.0	0.03374651491195249	0.0
20	0.0	1.4483482794829398E-4	0.0	0.03620870698707349	0.0
21	0.0	1.4483482794829398E-4	0.0	0.039974412513729136	0.0
22	0.0	1.4483482794829398E-4	0.0	0.046202310115505775	0.0
23	0.0	1.4483482794829398E-4	0.0	0.05479584324043788	0.0
24	4.827827598276466E-5	1.4483482794829398E-4	0.0	0.06623779464835311	0.0
25	4.827827598276466E-5	1.4483482794829398E-4	0.0	0.07063111776278469	0.0
26	4.827827598276466E-5	1.4483482794829398E-4	0.0	0.07560378018900946	0.0
27	4.827827598276466E-5	1.4483482794829398E-4	0.0	0.08424559158992433	0.0
28	4.827827598276466E-5	1.4483482794829398E-4	0.0	0.10500525026251313	0.0
29	4.827827598276466E-5	1.4483482794829398E-4	0.0	0.1342618855080685	0.0
30	4.827827598276466E-5	1.4483482794829398E-4	0.0	0.18210565700698828	0.0
31	4.827827598276466E-5	1.4483482794829398E-4	0.0	0.3675425150567873	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TATACGG	45	4.0103005E-6	28.777777	2
CGAATTA	135	5.638867E-11	19.185186	15
GTATACG	50	0.007036742	18.499998	1
GTATAGA	185	0.0	18.0	1
GTAAGAC	150	4.6766218E-9	16.033333	3
CTACACG	70	0.0025934346	15.857143	4
GGTATCA	3065	0.0	15.753671	1
CTTATAC	1030	0.0	15.626213	37
GCTTAGG	1450	0.0	15.055173	1
ATCTCGC	1095	0.0	14.86758	11
TCTTATA	1620	0.0	14.845679	37
TAGGACA	1535	0.0	14.824104	4
CGAACTA	75	0.004105883	14.8	29
TCTATAC	125	2.9613348E-6	14.799999	3
CGGTGAT	240	0.0	14.645834	14
TTAGGAC	1595	0.0	14.614419	3
TAATACT	280	0.0	14.535715	4
GGTATAT	115	2.211426E-5	14.478261	1
GGTCTAC	115	2.211426E-5	14.478261	1
CGGCCTT	1125	0.0	14.471111	24
>>END_MODULE