FastQCFastQC Report
Fri 10 Feb 2017
ERR1630581.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1630581.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences974971
Sequences flagged as poor quality0
Sequence length43
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGTAGGCACACGCTGAGCCAGTCAGTGTAGCGCGCGTGCAGC9760.10010554160072455No Hit
GTGTAGCGCGCGTGCAGCCCCGGACATCTAAGGGCATCACAGA9750.1000029744474451No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTACG250.005495939429.631
TATACCG400.001930977823.1250025
GGTATCA3750.022.6933351
TAATACT1155.456968E-1222.521744
TTAGTCG450.003825397720.5555555
CTAGATC555.142325E-420.1818183
GTAGCAC951.675453E-719.4736843
CTAATAC1457.2759576E-1219.137933
CTAGATA902.1515334E-618.53
GCGAACG500.007034135518.526
ATAATAC500.007034135518.53
TGTATCG609.2354463E-418.52
ATATACT902.1515334E-618.54
TATCGAC609.2354463E-418.54
ATCGACG609.2354463E-418.55
TAGTACA701.2189569E-418.54
TATACTG1054.79542E-717.619055
TATAACA953.6052515E-617.5263164
CGATTTT752.0668609E-417.26666621
TCTAATA752.0668609E-417.2666662
Produced by FastQC (version 0.11.5)