##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1630581.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	974971
Sequences flagged as poor quality	0
Sequence length	43
%GC	53
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.77452765261736	31.0	31.0	34.0	30.0	34.0
2	31.961772196301222	33.0	31.0	34.0	30.0	34.0
3	32.069088208777494	33.0	31.0	34.0	30.0	34.0
4	35.71482228702187	37.0	35.0	37.0	33.0	37.0
5	35.64865519076978	37.0	35.0	37.0	33.0	37.0
6	35.7176726282115	37.0	35.0	37.0	33.0	37.0
7	35.66234688006105	37.0	35.0	37.0	33.0	37.0
8	35.642679628419714	37.0	35.0	37.0	33.0	37.0
9	37.34566053759548	39.0	37.0	39.0	34.0	39.0
10	37.189165626464785	39.0	37.0	39.0	34.0	39.0
11	37.31173542597677	39.0	37.0	39.0	34.0	39.0
12	37.222075323266026	39.0	37.0	39.0	34.0	39.0
13	37.28530387057666	39.0	37.0	39.0	34.0	39.0
14	38.52245553970323	40.0	38.0	41.0	34.0	41.0
15	38.5315870933597	40.0	38.0	41.0	34.0	41.0
16	38.498826118930715	40.0	38.0	41.0	34.0	41.0
17	38.43842739937906	40.0	38.0	41.0	34.0	41.0
18	38.41447694341678	40.0	38.0	41.0	34.0	41.0
19	38.45817465339995	40.0	38.0	41.0	34.0	41.0
20	38.41238559916141	40.0	38.0	41.0	34.0	41.0
21	38.36400980131717	40.0	38.0	41.0	34.0	41.0
22	38.29650010102865	40.0	38.0	41.0	34.0	41.0
23	38.23732808463021	40.0	38.0	41.0	34.0	41.0
24	38.17999817430467	40.0	38.0	41.0	33.0	41.0
25	38.119500990285864	40.0	38.0	41.0	33.0	41.0
26	37.992866454489416	40.0	37.0	41.0	33.0	41.0
27	37.85527364403659	40.0	37.0	41.0	33.0	41.0
28	37.74729197073554	40.0	37.0	41.0	32.0	41.0
29	37.68011766503824	40.0	37.0	41.0	32.0	41.0
30	37.60023836606422	40.0	37.0	41.0	32.0	41.0
31	37.5801772565543	40.0	37.0	41.0	32.0	41.0
32	37.49853893090153	40.0	37.0	41.0	32.0	41.0
33	37.429443542423314	39.0	37.0	41.0	32.0	41.0
34	37.38511094176135	39.0	37.0	41.0	31.0	41.0
35	37.36214205345595	39.0	36.0	41.0	31.0	41.0
36	37.35047914245654	39.0	36.0	41.0	31.0	41.0
37	37.2349321159296	39.0	36.0	41.0	31.0	41.0
38	37.09618645067392	39.0	36.0	41.0	31.0	41.0
39	37.06727174449291	39.0	36.0	40.0	31.0	41.0
40	36.91704881478526	39.0	35.0	40.0	31.0	41.0
41	36.88039439121779	39.0	35.0	40.0	31.0	41.0
42	36.768737736814735	39.0	35.0	40.0	30.0	41.0
43	35.88023028377254	38.0	34.0	40.0	29.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
9	1.0
10	3.0
11	2.0
12	0.0
13	2.0
14	3.0
15	2.0
16	3.0
17	12.0
18	26.0
19	58.0
20	119.0
21	271.0
22	499.0
23	886.0
24	1610.0
25	2579.0
26	3800.0
27	5677.0
28	8256.0
29	11392.0
30	15578.0
31	20433.0
32	26597.0
33	34527.0
34	45442.0
35	62048.0
36	87757.0
37	136466.0
38	259682.0
39	251240.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	44.27670156343112	17.319694637071255	11.130690041037118	27.27291375846051
2	20.698769501862106	18.842714296117524	32.58722567132766	27.871290530692704
3	21.151090647824397	20.611382287268032	27.974883355504932	30.26264370940264
4	15.661901738615816	14.199704401464249	31.837870049468137	38.3005238104518
5	16.364486738579917	34.82226650843974	33.30242643114513	15.510820321835213
6	39.46096858265528	32.10844219981928	13.866463720459377	14.564125497066065
7	32.54425003410358	27.78441615186503	19.337908512150616	20.333425301880773
8	29.3902075036078	32.06515886113536	18.113872104913888	20.430761530342952
9	27.743389290553257	12.266313562146976	17.091790422484358	42.898506724815405
10	17.438877669181956	25.18536448776425	32.20188087645684	25.173876966596954
11	40.001600047591154	18.988667355234156	19.491656674916484	21.518075922258202
12	23.659472948426156	22.8439615127014	27.38973774604578	26.10682779282666
13	33.0203667596267	17.511084945090673	22.606415985706242	26.862132309576385
14	24.545550585607163	18.06351163265369	22.58692822658315	34.804009555156
15	27.80062176208318	25.682404912556372	19.873616753729085	26.64335657163136
16	28.555721144526352	23.555264720694257	21.12852587410292	26.760488260676475
17	26.39678513514761	24.21066883014982	22.611031507603816	26.781514527098754
18	26.92428800446372	22.750215134604005	23.70993598783964	26.615560873092637
19	27.30645321758288	23.07504530903996	22.796062652119907	26.82243882125725
20	27.251682357731667	22.88775768715172	23.143355033124063	26.71720492199255
21	28.15960679856119	22.64990445869672	22.714008929496366	26.476479813245728
22	27.942984970835028	22.762112924384418	22.509797727317018	26.785104377463536
23	26.989725848256	22.519336472572004	23.2079723396901	27.28296533948189
24	27.15444869642277	22.97083708130806	22.686828633877315	27.187885588391858
25	27.732414605152357	22.731650479860427	22.710008810518467	26.82592610446875
26	27.490766392026018	22.97976042364337	22.71790648132098	26.811566703009625
27	27.78441615186503	22.51472095067443	22.7981139951855	26.902748902275043
28	26.912185080376748	22.72406051051775	23.112995155753353	27.250759253352154
29	26.69422987965796	22.97996555794993	23.208074906843382	27.11772965554873
30	26.498018915434407	23.343463549172235	23.393721454279152	26.764796081114206
31	27.30870969495503	23.30264182216702	22.47513002950857	26.913518453369385
32	26.875568606655992	22.818319724381546	23.009402330941125	27.296709338021337
33	26.358014751207985	22.663033054316486	23.3745413966159	27.60441079785963
34	27.328197454078122	22.31512527039266	23.17597138786692	27.1807058876623
35	26.720589638050775	22.382204188637406	24.038150878333816	26.859055294978006
36	26.495352169449145	22.879962583502483	23.621830803172607	27.00285444387577
37	27.029316769421857	22.081784996681954	24.044099773224023	26.844798460672166
38	26.00344010232099	22.07347705726632	24.730991998736375	27.192090841676315
39	26.252268016176895	22.047322433180064	24.680221257863057	27.02018829277999
40	26.058416096478766	21.88967671858958	25.05284772572723	26.999059459204428
41	25.236442930097407	21.829059531001434	25.91307844028181	27.021419098619344
42	25.46403944322447	21.603001525173568	26.17462468114436	26.758334350457602
43	24.664836184871138	21.201451120084595	26.25903744829333	27.87467524675093
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	10.0
1	10.5
2	11.0
3	25.5
4	40.0
5	40.0
6	52.5
7	65.0
8	64.5
9	64.0
10	81.5
11	99.0
12	99.0
13	180.5
14	262.0
15	438.5
16	615.0
17	709.0
18	803.0
19	803.0
20	1113.5
21	1424.0
22	1806.5
23	2189.0
24	2789.5
25	3390.0
26	3390.0
27	4345.5
28	5301.0
29	6686.5
30	8072.0
31	10193.5
32	12315.0
33	12315.0
34	15241.5
35	18168.0
36	21680.0
37	25192.0
38	29869.5
39	34547.0
40	34547.0
41	38772.0
42	42997.0
43	46495.0
44	49993.0
45	54865.5
46	59738.0
47	59738.0
48	64019.0
49	68300.0
50	73083.0
51	77866.0
52	80376.5
53	82887.0
54	82887.0
55	83847.0
56	84807.0
57	81726.5
58	78646.0
59	77540.0
60	76434.0
61	76434.0
62	72522.5
63	68611.0
64	61383.0
65	54155.0
66	48482.5
67	42810.0
68	42810.0
69	36250.0
70	29690.0
71	25055.5
72	20421.0
73	15766.0
74	11111.0
75	11111.0
76	8686.5
77	6262.0
78	4898.5
79	3535.0
80	2904.5
81	2274.0
82	2274.0
83	1608.5
84	943.0
85	714.0
86	485.0
87	378.0
88	271.0
89	271.0
90	205.0
91	139.0
92	80.5
93	22.0
94	13.5
95	5.0
96	5.0
97	3.0
98	1.0
99	1.0
100	1.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	974971.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	62.010634570446385
#Duplication Level	Percentage of deduplicated	Percentage of total
1	85.1970849558755	52.8312530166607
2	8.118596986426887	10.068787019000903
3	2.475802466619853	4.605782460785205
4	1.1844998505008624	2.9380634951262947
5	0.677793681381369	2.101520814514882
6	0.4371026490962199	1.626300758572785
7	0.29903327103802124	1.298027002632079
8	0.22798260507936002	1.1309876809595671
9	0.18034519000235763	1.0064987706396527
>10	1.0557443804104083	12.200439162151245
>50	0.09602083509472849	4.079570296658712
>100	0.048166137201153286	5.358444873877274
>500	0.0018269912732957283	0.7543246484206857
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GGGTAGGCACACGCTGAGCCAGTCAGTGTAGCGCGCGTGCAGC	976	0.10010554160072455	No Hit
GTGTAGCGCGCGTGCAGCCCCGGACATCTAAGGGCATCACAGA	975	0.1000029744474451	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	1.0256715327943088E-4	0.0
3	0.0	0.0	0.0	1.0256715327943088E-4	0.0
4	0.0	0.0	0.0	2.0513430655886176E-4	0.0
5	0.0	0.0	0.0	2.0513430655886176E-4	0.0
6	0.0	0.0	0.0	2.0513430655886176E-4	0.0
7	0.0	0.0	0.0	2.0513430655886176E-4	0.0
8	0.0	0.0	0.0	2.0513430655886176E-4	0.0
9	0.0	0.0	0.0	7.179700729560161E-4	0.0
10	0.0	0.0	0.0	0.0011282386860737396	0.0
11	0.0	0.0	0.0	0.0011282386860737396	0.0
12	0.0	0.0	0.0	0.0012308058393531705	0.0
13	0.0	0.0	0.0	0.0013333729926326013	0.0
14	0.0	0.0	0.0	0.0014359401459120322	0.0
15	0.0	0.0	0.0	0.0019487759123091866	0.0
16	0.0	0.0	0.0	0.0022564773721474793	1.0256715327943088E-4
17	0.0	0.0	0.0	0.002461611678706341	1.0256715327943088E-4
18	0.0	0.0	0.0	0.002564178831985772	1.0256715327943088E-4
19	0.0	0.0	0.0	0.0026667459852652026	1.0256715327943088E-4
20	0.0	0.0	0.0	0.0029744474451034956	1.0256715327943088E-4
21	0.0	0.0	0.0	0.0035898503647800806	1.0256715327943088E-4
22	0.0	0.0	0.0	0.004307820437736097	1.0256715327943088E-4
23	0.0	2.0513430655886176E-4	0.0	0.00584632773692756	1.0256715327943088E-4
24	0.0	2.0513430655886176E-4	0.0	0.007487402189398454	1.0256715327943088E-4
25	0.0	2.0513430655886176E-4	0.0	0.0083079394156339	1.0256715327943088E-4
26	0.0	2.0513430655886176E-4	0.0	0.010051581021384226	1.0256715327943088E-4
27	0.0	2.0513430655886176E-4	0.0	0.015385072991914632	1.0256715327943088E-4
28	0.0	2.0513430655886176E-4	0.0	0.035385667881403654	1.0256715327943088E-4
29	0.0	2.0513430655886176E-4	0.0	0.07292524598167535	1.0256715327943088E-4
30	0.0	2.0513430655886176E-4	0.0	0.12779867298617087	1.0256715327943088E-4
31	0.0	2.0513430655886176E-4	0.0	0.3408306503475488	1.0256715327943088E-4
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGTTACG	25	0.0054959394	29.6	31
TATACCG	40	0.0019309778	23.125002	5
GGTATCA	375	0.0	22.693335	1
TAATACT	115	5.456968E-12	22.52174	4
TTAGTCG	45	0.0038253977	20.555555	5
CTAGATC	55	5.142325E-4	20.181818	3
GTAGCAC	95	1.675453E-7	19.473684	3
CTAATAC	145	7.2759576E-12	19.13793	3
CTAGATA	90	2.1515334E-6	18.5	3
GCGAACG	50	0.0070341355	18.5	26
ATAATAC	50	0.0070341355	18.5	3
TGTATCG	60	9.2354463E-4	18.5	2
ATATACT	90	2.1515334E-6	18.5	4
TATCGAC	60	9.2354463E-4	18.5	4
ATCGACG	60	9.2354463E-4	18.5	5
TAGTACA	70	1.2189569E-4	18.5	4
TATACTG	105	4.79542E-7	17.61905	5
TATAACA	95	3.6052515E-6	17.526316	4
CGATTTT	75	2.0668609E-4	17.266666	21
TCTAATA	75	2.0668609E-4	17.266666	2
>>END_MODULE