FastQCFastQC Report
Fri 10 Feb 2017
ERR1630580.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1630580.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1400713
Sequences flagged as poor quality0
Sequence length43
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT26480.18904657842113268No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT25970.18540557558900359No Hit
ACCTAGGCCTGGTCAGCATGTTGATGTATCTACGGAGATCAGC24710.17641015682727298No Hit
GTGTGGGAGCAGGGAGCAAGCTTTGGCCAGAGCCAAGGGTGCA22110.1578481816046542No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT22050.15741982833028606No Hit
GGCCTAGGTATGGGAAAAGACACAAAGAGGACACGCTGGCCTT21230.1515656669139217No Hit
GCTTTATTGAGCCTGTGTGGGAGCAGGGAGCAAGCTTTGGCCA20580.146925173108267No Hit
GTATGCAGCTGATCTCCGTAGATACATCAACATGCTGACCAGG16350.11672626726531415No Hit
GGGTACACTGGCTCCAGTGGGGCTCCCTGGGCACCCAGCAGTG14420.10294757027313947No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA15350.019.0423451
GCTTTAT7200.018.2430571
CGAATTA852.7238033E-517.41176415
TCTATAC1107.810595E-716.8181823
TTTATTG9150.016.3770483
GTAAACG1951.8189894E-1216.12820627
TATTGAG7700.016.0974045
TCTTGCG1751.3096724E-1015.8571422
ACGCTTC2150.015.48837431
TTTCGGA2800.015.19642830
ACCGAGT750.004105001714.88
ACTCGAA750.004105001714.827
GTATACT750.004105001714.84
TAGACTC1001.0936351E-414.7999995
CGCTTCG2301.8189894E-1214.47826132
CTTTATT9400.014.3670212
TCTTATA13450.014.30483237
CGATTAA1304.4453445E-614.23076924
TTATTGA9000.014.1833334
AAAGCCG2353.6379788E-1214.17021215
Produced by FastQC (version 0.11.5)