FastQCFastQC Report
Fri 10 Feb 2017
ERR1630579.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1630579.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences800842
Sequences flagged as poor quality0
Sequence length43
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT72450.9046728318444837No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT59810.7468389520030168No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT57720.7207414196558123No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT45930.5735213687593808No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT14120.17631442906341077No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT13870.17319271466781214No Hit
AACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT12680.15833335414476266No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT9790.12224633573164245No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTT9600.11987383279098748No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCTAGA701.9214167E-723.7857151
GGACCGT450.003824810520.5555556
ACTATGC701.21861136E-418.58
CTAGTGC609.233479E-418.53
AAATGCG500.007033066418.49999834
CGGACCA852.7214464E-517.4117649
AAGACGG752.0662801E-417.2666665
ACGGACC752.0662801E-417.2666668
TAACCCG1757.2759576E-1216.91428628
TAGTAGT2050.016.2439044
CGCTCGC803.3809635E-416.187518
GACGGAC803.3809635E-416.18757
ACTGTAC1403.4680852E-815.8571433
GTCTAGT1059.335947E-615.8571421
CGAAAGC1101.4506966E-515.13636419
TATCTCG1853.0377123E-1015.036
TCCGGTC1252.9558487E-614.8000018
CTAGATT750.004102960714.823
CGGGTCG750.004102960714.834
GACACGC750.004102960714.830
Produced by FastQC (version 0.11.5)