FastQCFastQC Report
Fri 10 Feb 2017
ERR1630578.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1630578.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1088897
Sequences flagged as poor quality0
Sequence length43
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT42430.3896603627340327No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT41050.37698698774998923No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT30140.27679385653555844No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT19050.17494767641016554No Hit
ACCTAGGCCTGGTCAGCATGTTGATGTATCTACGGAGATCAGC16120.14803971358172535No Hit
GTGTGGGAGCAGGGAGCAAGCTTTGGCCAGAGCCAAGGGTGCA15790.145009123911628No Hit
GGCCTAGGTATGGGAAAAGACACAAAGAGGACACGCTGGCCTT14660.1346316501928098No Hit
GCTTTATTGAGCCTGTGTGGGAGCAGGGAGCAAGCTTTGGCCA13630.12517253698008168No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGACGG2200.022.7045465
CGCAAGA2450.022.6530632
CAAGACG2550.021.7647044
ACGGACC2400.020.8124988
GACGGAC2500.019.987
CGGACCA2550.019.5882349
GCGCAAG2850.018.824561
TCAGAAC1451.546141E-1017.8620683
ACGAACG1156.403934E-817.69565215
ACCGTCG2000.017.5758
AGACGGA2850.017.5263166
CCGTCGT2050.017.1463419
AGCCCGT650.001579861217.0769256
ATACCGT2200.016.8181826
TACCGTC2000.016.6500027
GCTTTAT4700.016.5319141
GGTATCA22000.016.481821
TAACGAA1251.657354E-716.27999913
CGTCGTA2050.016.24390210
CGAAAGC3200.016.187519
Produced by FastQC (version 0.11.5)