FastQCFastQC Report
Fri 10 Feb 2017
ERR1630577.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1630577.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1336624
Sequences flagged as poor quality0
Sequence length43
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT95750.7163570308478674No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT87980.6582254994673147No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT69340.5187696764385497No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT45620.3413076527131041No Hit
GTGTAGCGCGCGTGCAGCCCCGGACATCTAAGGGCATCACAGA25360.18973174206059445No Hit
GGGTAGGCACACGCTGAGCCAGTCAGTGTAGCGCGCGTGCAGC21560.16130190689378612No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAATGTTTT17720.13257281030416931No Hit
TGCCTACCCTACGCCGGCAGGCGCGGGTAACCCGTTGAACCCC17390.13010390356599913No Hit
GTACATGGGGAATAATTGCAATCCCCGATCCCCATCACGAATG16590.12411867510982895No Hit
GTGCATGGCCGTTCTTAGTTGGTGGAGCGATTTGTCTGGTTAA15950.1193304923448928No Hit
GGGTTCAACGGGTTACCCGCGCCTGCCGGCGTAGGGTAGGCAC14700.1099785728821269No Hit
GCGCTACACTGACTGGCTCAGCGTGTGCCTACCCTACGCCGGC13610.10182369911059506No Hit
GGATTGACAGATTGATAGCTCTTTCTCGATTCCGTGGGTGGTG13470.10077628413076528No Hit
ACCCCCGAGCGGTCGGCGTCCCCCAACTTCTTAGAGGGACAAG13430.10047702270795678No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCGCTTA903.8289727E-922.61111325
GCCGTCT1202.382876E-1020.04166836
AAGACGG3700.018.55
CGCTTAT1103.8529834E-818.526
ATGCCGT1054.7987123E-717.61904734
CGCAAGA4500.016.8555562
TACACCA3100.016.7096795
AGTCGGT3950.016.39240511
ATTGCCG2750.016.14545411
ACCATTG2900.015.9482768
CAAGACG4300.015.9186044
GCGCAAG4650.015.9139791
GGTATCA44200.015.8631221
ACGGGAC957.061048E-515.5789483
CCGCTAC2850.015.5789471
GTATATC1557.210474E-915.51612951
TCGGTGA4450.015.38202213
CGAGCCG4100.015.34146415
GTCCTAT3500.015.3285711
GCAGTCG4350.015.3103469
Produced by FastQC (version 0.11.5)