##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1630577.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1336624
Sequences flagged as poor quality	0
Sequence length	43
%GC	48
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.98397829157639	33.0	31.0	34.0	30.0	34.0
2	32.13047349142317	33.0	31.0	34.0	30.0	34.0
3	32.21565002573648	34.0	31.0	34.0	30.0	34.0
4	35.82050823567435	37.0	35.0	37.0	35.0	37.0
5	35.80716865775267	37.0	35.0	37.0	35.0	37.0
6	35.8526870683154	37.0	35.0	37.0	35.0	37.0
7	35.82679646632112	37.0	35.0	37.0	35.0	37.0
8	35.834229371910126	37.0	35.0	37.0	35.0	37.0
9	37.54004192652533	39.0	37.0	39.0	35.0	39.0
10	37.45191018566179	39.0	37.0	39.0	35.0	39.0
11	37.56042013311148	39.0	37.0	39.0	35.0	39.0
12	37.49170372520619	39.0	37.0	39.0	35.0	39.0
13	37.541573396856556	39.0	37.0	39.0	35.0	39.0
14	38.778410383174325	40.0	38.0	41.0	35.0	41.0
15	38.74636247740576	40.0	38.0	41.0	35.0	41.0
16	38.71062617460108	40.0	38.0	41.0	35.0	41.0
17	38.72376449921593	40.0	38.0	41.0	35.0	41.0
18	38.720706047474835	40.0	38.0	41.0	35.0	41.0
19	38.7527330049438	40.0	38.0	41.0	35.0	41.0
20	38.74234040388322	40.0	38.0	41.0	35.0	41.0
21	38.66571900549444	40.0	38.0	41.0	34.0	41.0
22	38.59288850117909	40.0	38.0	41.0	34.0	41.0
23	38.54663989274471	40.0	38.0	41.0	34.0	41.0
24	38.476045619411295	40.0	38.0	41.0	34.0	41.0
25	38.43452983037862	40.0	38.0	41.0	34.0	41.0
26	38.22302981242294	40.0	38.0	41.0	34.0	41.0
27	38.10067453524701	40.0	37.0	41.0	33.0	41.0
28	37.95982565029507	40.0	37.0	41.0	33.0	41.0
29	37.83964301104873	40.0	37.0	41.0	33.0	41.0
30	37.72505730856247	40.0	37.0	41.0	33.0	41.0
31	37.65562117693533	40.0	37.0	41.0	33.0	41.0
32	37.518843743640694	40.0	36.0	41.0	32.0	41.0
33	37.4276752474892	40.0	36.0	41.0	32.0	41.0
34	37.33884099043561	40.0	36.0	41.0	32.0	41.0
35	37.23131860568118	40.0	36.0	41.0	32.0	41.0
36	37.16042133015717	39.0	36.0	41.0	32.0	41.0
37	36.94036467996983	39.0	35.0	41.0	31.0	41.0
38	36.78394372688206	39.0	35.0	41.0	31.0	41.0
39	36.672301260489114	39.0	35.0	41.0	30.0	41.0
40	36.503557470163635	39.0	35.0	41.0	30.0	41.0
41	36.32389288236632	39.0	35.0	41.0	30.0	41.0
42	36.16259247177965	39.0	35.0	41.0	30.0	41.0
43	35.29068758304504	38.0	34.0	40.0	26.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	1.0
9	5.0
10	8.0
11	4.0
12	1.0
13	0.0
14	4.0
15	4.0
16	7.0
17	27.0
18	41.0
19	115.0
20	272.0
21	454.0
22	859.0
23	1456.0
24	2292.0
25	3713.0
26	5482.0
27	7879.0
28	11604.0
29	15955.0
30	21154.0
31	27656.0
32	34994.0
33	45939.0
34	60971.0
35	81074.0
36	114429.0
37	180294.0
38	300076.0
39	419854.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	44.43313901291612	19.283807562934676	12.5633686062797	23.7196848178695
2	19.441593148110464	21.625453381055554	33.16220567639066	25.770747794443317
3	21.42158153676726	23.906049868923496	28.98316953758125	25.689199056727997
4	14.569243108009433	15.781401501095296	33.08095620009816	36.568399190797116
5	14.400010773411221	37.844749159075405	32.771295442847055	14.983944624666323
6	34.41596140724691	33.26634865152803	16.253261949508612	16.064427991716443
7	28.746977459629637	31.180646165264125	21.000221453452877	19.072154921653357
8	28.9531685799447	31.228976885047704	19.958941332790673	19.85891320221693
9	27.17181496067705	13.36314475873544	20.57826284729288	38.886777433294625
10	18.642864410634555	26.596035983193477	30.79093297741175	23.97016662876022
11	35.5304857611415	21.963020265983552	20.626443936365053	21.880050036509893
12	21.972521816157723	26.54927638587965	27.786797184548533	23.691404613414093
13	29.87646488466465	19.41578185039323	26.544413387759008	24.163339877183112
14	23.46965189911299	21.522357820898026	25.307042219801527	29.70094806018746
15	26.702273788290498	26.969813500281305	23.081210572307544	23.24670213912065
16	25.69436131627144	25.495651731526593	25.074890171057827	23.735096781144136
17	23.893480889165538	26.464360957157734	25.525128981673227	24.117029172003495
18	23.506386238762733	23.66095476364333	28.156160595649936	24.676498401944002
19	23.635592358060308	25.27352494044698	27.819042602856154	23.271840098636567
20	24.268679898011708	23.46336740923401	28.03570787296951	24.23224481978477
21	25.568521887980467	24.387785944289494	27.160218580543223	22.883473587186824
22	25.281829444929915	24.17164438166605	27.021585726427176	23.52494044697686
23	23.815298842456816	24.410903889201453	27.685946085062067	24.087851183279664
24	23.3821179329415	25.541363913860593	27.457160727324965	23.619357425872945
25	24.012961012221837	24.644103352924983	27.230919091681727	24.112016543171453
26	23.884503046481285	26.008136918086162	27.177351296999007	22.930008738433546
27	24.837650678126383	24.377910317336813	26.999365565783645	23.78507343875316
28	23.208695938423972	25.07840649277583	28.186535630065002	23.526361938735203
29	22.86297417972444	25.3623307676654	28.069898490525386	23.704796562084777
30	22.138537090460744	26.33979338991369	28.473527334538357	23.048142185087205
31	23.417580411544307	26.140036390189014	26.84337554914471	23.599007649121965
32	22.985970624498737	25.431534971689867	27.074405367552878	24.508089036258514
33	22.410116831659465	25.563060367014206	27.763005901435257	24.26381689989107
34	23.862357701193453	25.43579944696489	27.227702031386535	23.474140820455116
35	23.24206358706712	25.267315265923703	28.38038221668921	23.11023893031997
36	22.19434934581453	26.302236081351225	27.558385903589937	23.945028669244305
37	23.590478698571925	25.344375082296892	27.43060127605071	23.634544943080478
38	22.41939393576653	24.815430517482852	28.235240426627083	24.529935120123536
39	23.326829385077627	24.879547277319574	27.664698524042663	24.128924813560136
40	22.283978141945678	25.08536432085613	28.6086438669364	24.022013670261792
41	21.566648511473684	25.62223931337459	29.108335627670908	23.702776547480816
42	22.809630831108823	25.28384945953387	28.79119333484959	23.115326374507713
43	21.739322352434193	24.505919390943152	28.709494966422866	25.045263290199788
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	360.0
1	345.5
2	331.0
3	859.5
4	1388.0
5	1388.0
6	2027.5
7	2667.0
8	2637.0
9	2607.0
10	3670.5
11	4734.0
12	4734.0
13	8320.0
14	11906.0
15	16416.0
16	20926.0
17	19440.5
18	17955.0
19	17955.0
20	19994.0
21	22033.0
22	18318.5
23	14604.0
24	15732.5
25	16861.0
26	16861.0
27	19070.0
28	21279.0
29	25206.5
30	29134.0
31	32548.5
32	35963.0
33	35963.0
34	39481.5
35	43000.0
36	47785.0
37	52570.0
38	57769.0
39	62968.0
40	62968.0
41	67170.5
42	71373.0
43	72968.5
44	74564.0
45	78182.5
46	81801.0
47	81801.0
48	86535.0
49	91269.0
50	90338.5
51	89408.0
52	92467.0
53	95526.0
54	95526.0
55	90965.5
56	86405.0
57	82395.5
58	78386.0
59	73691.5
60	68997.0
61	68997.0
62	66064.5
63	63132.0
64	58621.5
65	54111.0
66	47830.5
67	41550.0
68	41550.0
69	35166.0
70	28782.0
71	25060.5
72	21339.0
73	16099.0
74	10859.0
75	10859.0
76	8497.0
77	6135.0
78	5187.5
79	4240.0
80	3537.5
81	2835.0
82	2835.0
83	2273.0
84	1711.0
85	1490.0
86	1269.0
87	1150.0
88	1031.0
89	1031.0
90	782.0
91	533.0
92	294.5
93	56.0
94	35.5
95	15.0
96	15.0
97	12.5
98	10.0
99	5.5
100	1.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	1336624.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	49.47816067053648
#Duplication Level	Percentage of deduplicated	Percentage of total
1	83.58912934388631	41.358363719870624
2	9.828101898292163	9.725528096202085
3	2.7215013716969207	4.03964646401717
4	1.102688109282355	2.1823591776224984
5	0.5979375778992349	1.479242577512488
6	0.3556519094762663	1.0558201391909878
7	0.24456502856019632	0.8470439444247018
8	0.1767563335769403	0.6996462617803835
9	0.12805640416604797	0.5702395806196991
>10	0.9479724576919485	9.632883868961688
>50	0.1521848060032244	5.30811921888296
>100	0.1372551825467586	13.581215570088462
>500	0.013194693268314288	4.356379857347166
>1k	0.004549894230453203	3.2644708880382707
>5k	4.5498942304532034E-4	1.8990406354408782
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	9575	0.7163570308478674	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	8798	0.6582254994673147	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	6934	0.5187696764385497	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	4562	0.3413076527131041	No Hit
GTGTAGCGCGCGTGCAGCCCCGGACATCTAAGGGCATCACAGA	2536	0.18973174206059445	No Hit
GGGTAGGCACACGCTGAGCCAGTCAGTGTAGCGCGCGTGCAGC	2156	0.16130190689378612	No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAATGTTTT	1772	0.13257281030416931	No Hit
TGCCTACCCTACGCCGGCAGGCGCGGGTAACCCGTTGAACCCC	1739	0.13010390356599913	No Hit
GTACATGGGGAATAATTGCAATCCCCGATCCCCATCACGAATG	1659	0.12411867510982895	No Hit
GTGCATGGCCGTTCTTAGTTGGTGGAGCGATTTGTCTGGTTAA	1595	0.1193304923448928	No Hit
GGGTTCAACGGGTTACCCGCGCCTGCCGGCGTAGGGTAGGCAC	1470	0.1099785728821269	No Hit
GCGCTACACTGACTGGCTCAGCGTGTGCCTACCCTACGCCGGC	1361	0.10182369911059506	No Hit
GGATTGACAGATTGATAGCTCTTTCTCGATTCCGTGGGTGGTG	1347	0.10077628413076528	No Hit
ACCCCCGAGCGGTCGGCGTCCCCCAACTTCTTAGAGGGACAAG	1343	0.10047702270795678	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.00104741497982978	0.0
3	0.0	0.0	0.0	0.0016459378254467974	0.0
4	0.0	0.0	0.0	0.0028429835166808317	0.0
5	0.0	0.0	0.0	0.0028429835166808317	0.0
6	0.0	0.0	0.0	0.002917798872382959	0.0
7	0.0	0.0	0.0	0.0033666910065957216	0.0
8	0.0	0.0	0.0	0.003516321717999976	0.0
9	0.0	0.0	0.0	0.005760782389063791	0.0
10	0.0	0.0	0.0	0.008080058415829733	0.0
11	7.481535570212714E-5	0.0	0.0	0.009127473395659513	0.0
12	7.481535570212714E-5	0.0	0.0	0.010025257664085038	0.0
13	7.481535570212714E-5	0.0	0.0	0.010997857288212691	0.0
14	7.481535570212714E-5	0.0	0.0	0.011671195489531836	0.0
15	7.481535570212714E-5	0.0	0.0	0.013766025449191395	0.0
16	7.481535570212714E-5	0.0	0.0	0.018554208214127534	0.0
17	1.496307114042543E-4	0.0	0.0	0.024464621314595578	0.0
18	1.496307114042543E-4	7.481535570212714E-5	0.0	0.026858712697063646	0.0
19	1.496307114042543E-4	7.481535570212714E-5	0.0	0.030300219059361495	0.0
20	1.496307114042543E-4	7.481535570212714E-5	0.0	0.03269431044182956	0.0
21	1.496307114042543E-4	7.481535570212714E-5	0.0	0.038903984965106116	0.0
22	1.496307114042543E-4	7.481535570212714E-5	0.0	0.05050036509893582	0.0
23	1.496307114042543E-4	7.481535570212714E-5	0.0	0.06591232837357403	0.0
24	1.496307114042543E-4	7.481535570212714E-5	0.0	0.0923221489364249	0.0
25	1.496307114042543E-4	7.481535570212714E-5	0.0	0.09927997701672273	0.0
26	1.496307114042543E-4	7.481535570212714E-5	0.0	0.11080154179485031	0.0
27	1.496307114042543E-4	7.481535570212714E-5	0.0	0.1278594428949353	0.0
28	1.496307114042543E-4	7.481535570212714E-5	0.0	0.16182561438370102	0.0
29	1.496307114042543E-4	7.481535570212714E-5	0.0	0.21644082404625384	0.0
30	1.496307114042543E-4	7.481535570212714E-5	0.0	0.2979147464058703	0.0
31	1.496307114042543E-4	7.481535570212714E-5	0.0	0.556027723578209	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CCGCTTA	90	3.8289727E-9	22.611113	25
GCCGTCT	120	2.382876E-10	20.041668	36
AAGACGG	370	0.0	18.5	5
CGCTTAT	110	3.8529834E-8	18.5	26
ATGCCGT	105	4.7987123E-7	17.619047	34
CGCAAGA	450	0.0	16.855556	2
TACACCA	310	0.0	16.709679	5
AGTCGGT	395	0.0	16.392405	11
ATTGCCG	275	0.0	16.145454	11
ACCATTG	290	0.0	15.948276	8
CAAGACG	430	0.0	15.918604	4
GCGCAAG	465	0.0	15.913979	1
GGTATCA	4420	0.0	15.863122	1
ACGGGAC	95	7.061048E-5	15.578948	3
CCGCTAC	285	0.0	15.578947	1
GTATATC	155	7.210474E-9	15.5161295	1
TCGGTGA	445	0.0	15.382022	13
CGAGCCG	410	0.0	15.341464	15
GTCCTAT	350	0.0	15.328571	1
GCAGTCG	435	0.0	15.310346	9
>>END_MODULE