FastQCFastQC Report
Fri 10 Feb 2017
ERR1630576.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1630576.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences203624
Sequences flagged as poor quality0
Sequence length43
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT59962.9446430676148196No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT57892.8429851098102388No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT40942.010568498801713No Hit
CTTATACACATCTCCGAGCCCACGAGACAGGCAGAAATCTCGT25451.2498526696263703No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT23691.1634188504302045No Hit
TCTCCGAGCCCACGAGACAGGCAGAAATCTCGTATGCCGTCTT18740.9203237339409892RNA PCR Primer, Index 17 (95% over 21bp)
ATACACATCTCCGAGCCCACGAGACAGGCAGAAATCTCGTATG12070.5927592032373394No Hit
ACACATCTCCGAGCCCACGAGACAGGCAGAAATCTCGTATGCC11250.5524889011118532No Hit
CATCTCCGAGCCCACGAGACAGGCAGAAATCTCGTATGCCGTC9870.4847169292421326No Hit
CTCTATGGGCAGTCGGTGATTCCTCGCCTTATTGATATGCCCA6860.3368954543668723No Hit
GAACAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAA6630.3256001257219188No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT6400.3143047970769654No Hit
AACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT5570.27354339370604647No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT5350.2627391663065257No Hit
GTACATGGGAATGGTATCAACGCAAAAAAAAAAAAAAAAAAAA4910.24113071150748439No Hit
ACGCAGAGTACATGGGAAAAAAAAAAAAAAAAAAAAAAAAAAA4620.22688877538993438No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTT4550.2234510666719051No Hit
GGTATCAACGCAGAGTACATGGGAAAAAAAAAAAAAAAAAAAA4430.217557851726712No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT4130.20282481436372923No Hit
CTCCGAGCCCACGAGACAGGCAGAAATCTCGTATGCCGTCTTC3940.19349389070050682RNA PCR Primer, Index 17 (95% over 22bp)
TATCAACGCAGAGTACATGGGAAAAAAAAAAAAAAAAAAAAAA3830.18809177700074647No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTG3460.16992103091973443No Hit
GTACATGGGAACAGTGGTATCAACGCAAAAAAAAAAAAAAAAA3320.1630456134836758No Hit
GTACATGGGAAGCAGAGGTATCAACGCAAAAAAAAAAAAAAAA3230.15862570227478098No Hit
TCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTT3180.15617019604761717No Hit
CTCTATGGGCATATCAATAAGGCGAGGAATCACCGACTGCCCA2900.14241936117549994No Hit
GAATGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAA2680.13161513377597925No Hit
GTATCAACGCAGAGTACATGGGAAAAAAAAAAAAAAAAAAAAA2630.12915962754881546No Hit
CACATCTCCGAGCCCACGAGACAGGCAGAAATCTCGTATGCCG2630.12915962754881546No Hit
GTACATGGGAAGCAGTGGTATCAAAAAAAAAAAAAAAAAAAAA2550.1252308175853534No Hit
CTATGGGCATATCAATAAGGCGAGGAATCACCGACTGCCCATA2440.11982870388559305No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT2410.11835540014929477No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGG2410.11835540014929477No Hit
GTACATGGGCCTCTCTATGGGCATATCAATAAGGCGAGGAATC2310.11344438769496719No Hit
CAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT2250.11049778022237064No Hit
GGGCATATCAATAAGGCGAGGAATCACCGACTGCCCATAGAGC2220.10902447648607237No Hit
CTCTATGGGCAGTCGGTGATTCCTCCGCTTATTGATATGCCCA2210.1085333752406396No Hit
CCCATAGAGAGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAA2190.1075511727497741No Hit
ACGCAGAGTACATGGGCCTCTCTATGGGCATATCAATAAGGCG2190.1075511727497741No Hit
CTCTATGGGCAGTCGGTGATTCCTCGCCTTATTGATATGCCCC2110.10362236278631201No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTC2070.10165795780458099No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CAAGTCG200.00183838137.029
GACAAGT200.00183838137.027
GGGTAAT200.00183838137.011
GTTCACG200.00183838137.06
TATTGAC401.5915211E-632.37530
CCGTCTT2650.032.1132137
GCCGTCT3000.029.636
CTTGTTC250.005485858329.5999983
TGGGTAA250.005485858329.59999810
GGAAAAC502.702218E-729.5999988
AGTCGAG250.005485858329.59999831
ATTGACT405.9165155E-527.75000231
CGTCTTC358.8442455E-426.42857237
ATGGGTG451.3179949E-424.6666665
ATGCCCC802.697925E-823.12500237
ACAAGTC400.001925763123.12500228
TATACAC4100.023.0121963
TGCCGTC4050.021.92592635
CAGTGGC603.7069363E-521.58333426
ATGCCGT4050.021.46913534
Produced by FastQC (version 0.11.5)