##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1630576.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	203624
Sequences flagged as poor quality	0
Sequence length	43
%GC	42
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.89659372176168	33.0	31.0	34.0	30.0	34.0
2	31.967538207676895	33.0	31.0	34.0	30.0	34.0
3	31.961978941578597	33.0	31.0	34.0	30.0	34.0
4	35.6457097395199	37.0	35.0	37.0	33.0	37.0
5	35.69748163281342	37.0	35.0	37.0	35.0	37.0
6	35.791856559148236	37.0	35.0	37.0	35.0	37.0
7	35.73765371468982	37.0	35.0	37.0	35.0	37.0
8	35.80976211055671	37.0	35.0	37.0	35.0	37.0
9	37.43253251090245	39.0	37.0	39.0	34.0	39.0
10	37.36242780811692	39.0	37.0	39.0	34.0	39.0
11	37.51966369386713	39.0	37.0	39.0	35.0	39.0
12	37.45098318469336	39.0	37.0	39.0	35.0	39.0
13	37.5278601736534	39.0	37.0	39.0	35.0	39.0
14	38.57025203315916	40.0	38.0	41.0	34.0	41.0
15	38.51654520095863	40.0	38.0	41.0	34.0	41.0
16	38.3623983420422	40.0	38.0	41.0	34.0	41.0
17	38.49991160177582	40.0	38.0	41.0	34.0	41.0
18	38.63996876596079	40.0	38.0	41.0	34.0	41.0
19	38.70388068204141	40.0	38.0	41.0	35.0	41.0
20	38.75496012257887	40.0	38.0	41.0	35.0	41.0
21	38.680705614269435	40.0	38.0	41.0	35.0	41.0
22	38.567875103131264	40.0	38.0	41.0	34.0	41.0
23	38.49496621223432	40.0	38.0	41.0	34.0	41.0
24	38.40117078536911	40.0	38.0	41.0	34.0	41.0
25	38.30823969669587	40.0	37.0	41.0	34.0	41.0
26	37.9731613169371	40.0	37.0	41.0	33.0	41.0
27	37.72441362511295	40.0	36.0	41.0	33.0	41.0
28	37.55937414057282	40.0	35.0	41.0	33.0	41.0
29	37.30201253290378	39.0	35.0	41.0	32.0	41.0
30	37.02219777629356	39.0	35.0	41.0	32.0	41.0
31	36.695173456959886	39.0	35.0	40.0	31.0	41.0
32	36.374857580638825	38.0	35.0	40.0	31.0	41.0
33	36.11534003850234	38.0	35.0	40.0	31.0	41.0
34	35.835220799119945	38.0	35.0	40.0	30.0	41.0
35	35.53850724865438	38.0	35.0	40.0	29.0	41.0
36	35.22714414803756	37.0	35.0	40.0	27.0	41.0
37	34.73659784701214	37.0	34.0	40.0	24.0	41.0
38	34.36468196283346	37.0	34.0	40.0	22.0	41.0
39	33.97113306879346	36.0	33.0	40.0	21.0	41.0
40	33.50811790358701	35.0	33.0	40.0	18.0	41.0
41	33.053230463992456	35.0	33.0	40.0	15.0	41.0
42	32.594718697206616	35.0	33.0	40.0	10.0	41.0
43	31.554148823321416	35.0	31.0	39.0	10.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	1.0
9	1.0
10	3.0
11	1.0
12	1.0
13	1.0
14	1.0
15	2.0
16	1.0
17	4.0
18	12.0
19	18.0
20	70.0
21	120.0
22	217.0
23	381.0
24	604.0
25	956.0
26	1508.0
27	2203.0
28	3055.0
29	4129.0
30	5086.0
31	6166.0
32	7433.0
33	9205.0
34	11360.0
35	14466.0
36	20124.0
37	35152.0
38	40093.0
39	41250.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	47.769909244489845	18.150610929949316	14.41382155345146	19.66565827210938
2	18.244902369072406	23.173103366990137	33.49998035595018	25.08201390798727
3	23.042961536950457	26.730149687659612	31.01206144658783	19.214827328802105
4	13.244018386830628	17.857914587671395	28.36846344242329	40.52960358307469
5	11.977959376104979	42.22734058853573	29.939987427808116	15.854712607551171
6	30.49787844261973	29.335932895925826	18.988920755903038	21.177267905551407
7	25.451322044552704	35.13632970573213	19.353808981259576	20.058539268455586
8	35.119632263387416	28.086571327544885	18.8803873806624	17.913409028405297
9	28.566377244332692	10.632833064864652	20.777511491769143	40.02327819903351
10	21.32901819039013	29.97731112246101	23.989804738144816	24.703865949004047
11	29.586885632341964	24.293796409067696	18.154048638667348	27.965269319922996
12	21.367324087533888	32.23686795269713	25.494538954150787	20.901269005618197
13	33.53681294935764	17.714021922759596	28.235866106156443	20.51329902172632
14	24.332593407456883	22.801830825442973	28.629729304993518	24.23584646210663
15	31.26154087926767	22.69624405767493	25.524496130122188	20.517718932935214
16	23.45303107688681	23.65290928377794	29.957175971398264	22.936883667936982
17	18.35785565552194	31.497269477075395	28.655266569756023	21.489608297646644
18	20.06492358464621	21.22539582760382	32.996601579381604	25.713079008368368
19	19.732939142733667	28.1032687698896	34.53374847758614	17.630043609790594
20	21.879542686520253	21.573095509370212	36.51288649668016	20.034475307429382
21	25.56623973598397	21.625152241386083	33.50194476093191	19.30666326169803
22	23.06506109299493	24.58010843515499	31.79389462931678	20.560935842533297
23	21.33589360782619	25.160099006011077	32.279102659804344	21.22490472635839
24	20.732821278434763	26.630947236082193	33.89433465603269	18.74189682945036
25	18.787569245275606	27.979511256040546	32.89887243154049	20.334047067143363
26	22.015086630259695	26.446784269044905	32.65823282127843	18.879896279416965
27	18.945703846304955	28.501551879935565	32.32919498683849	20.223549286920992
28	18.379464110320985	27.358268180568107	34.46204769575296	19.800220013357954
29	18.14913762621302	28.532982359643267	34.294582171060384	19.02329784308333
30	17.930106470750008	29.061898400974346	36.10625466546183	16.901740462813812
31	18.902978037952302	28.89001296507288	32.6322044552705	19.574804541704317
32	16.42389895100774	30.12758810356343	34.58482300711115	18.863689938317684
33	16.683200408596235	29.92083447923624	35.318037166542254	18.07792794562527
34	18.57099359603976	30.431579774486305	31.762955250854517	19.234471378619418
35	16.63409028405296	31.042018622559226	34.15363611362118	18.17025497976663
36	16.74753467174793	31.306722193847484	31.432935213923702	20.512807920480885
37	16.648823321415943	30.987997485561625	32.52759988999332	19.835579303029114
38	16.794680391309473	28.3881074922406	33.83638470907162	20.980827407378307
39	18.465406828271718	28.734333870270696	32.477016461713745	20.32324283974384
40	16.387066357600283	28.30805798923506	34.86131300828979	20.44356264487487
41	15.93427100931128	29.904628138136957	32.12882567870192	22.03227517384984
42	16.349251561701962	28.735316072761563	34.118767925195456	20.79666444034102
43	15.021313794051782	29.94244293403528	33.89679016225985	21.139453109653086
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	6.0
1	28.0
2	50.0
3	284.5
4	519.0
5	519.0
6	855.5
7	1192.0
8	1268.5
9	1345.0
10	1886.0
11	2427.0
12	2427.0
13	4292.5
14	6158.0
15	9371.0
16	12584.0
17	11195.0
18	9806.0
19	9806.0
20	10881.5
21	11957.0
22	8501.0
23	5045.0
24	4528.5
25	4012.0
26	4012.0
27	4169.5
28	4327.0
29	4246.0
30	4165.0
31	4148.0
32	4131.0
33	4131.0
34	4042.0
35	3953.0
36	3835.0
37	3717.0
38	4082.5
39	4448.0
40	4448.0
41	5132.0
42	5816.0
43	6488.5
44	7161.0
45	8774.0
46	10387.0
47	10387.0
48	12787.0
49	15187.0
50	15960.0
51	16733.0
52	12719.5
53	8706.0
54	8706.0
55	9796.0
56	10886.0
57	10063.5
58	9241.0
59	8212.5
60	7184.0
61	7184.0
62	6986.5
63	6789.0
64	6621.5
65	6454.0
66	6151.5
67	5849.0
68	5849.0
69	5283.0
70	4717.0
71	4087.0
72	3457.0
73	2881.5
74	2306.0
75	2306.0
76	1864.0
77	1422.0
78	1106.0
79	790.0
80	596.0
81	402.0
82	402.0
83	299.0
84	196.0
85	137.0
86	78.0
87	46.0
88	14.0
89	14.0
90	10.0
91	6.0
92	3.0
93	0.0
94	0.5
95	1.0
96	1.0
97	0.5
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	203624.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	49.13377160657607
#Duplication Level	Percentage of deduplicated	Percentage of total
1	82.458	40.514725391350495
2	9.805	9.635132612049565
3	2.943	4.338020695144601
4	1.355	2.6630504210764228
5	0.762	1.871996698210548
6	0.483	1.4238967011585744
7	0.35500000000000004	1.2209742244234152
8	0.244	0.9590912217603647
9	0.166	0.7340585478022463
>10	1.228	11.338600473649558
>50	0.117	4.005876399084146
>100	0.06999999999999999	7.012863221406601
>500	0.006	1.9987618289555142
>1k	0.006	6.4925365800929615
>5k	0.002	5.790414983834989
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	5996	2.9446430676148196	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	5789	2.8429851098102388	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	4094	2.010568498801713	No Hit
CTTATACACATCTCCGAGCCCACGAGACAGGCAGAAATCTCGT	2545	1.2498526696263703	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	2369	1.1634188504302045	No Hit
TCTCCGAGCCCACGAGACAGGCAGAAATCTCGTATGCCGTCTT	1874	0.9203237339409892	RNA PCR Primer, Index 17 (95% over 21bp)
ATACACATCTCCGAGCCCACGAGACAGGCAGAAATCTCGTATG	1207	0.5927592032373394	No Hit
ACACATCTCCGAGCCCACGAGACAGGCAGAAATCTCGTATGCC	1125	0.5524889011118532	No Hit
CATCTCCGAGCCCACGAGACAGGCAGAAATCTCGTATGCCGTC	987	0.4847169292421326	No Hit
CTCTATGGGCAGTCGGTGATTCCTCGCCTTATTGATATGCCCA	686	0.3368954543668723	No Hit
GAACAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAA	663	0.3256001257219188	No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT	640	0.3143047970769654	No Hit
AACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	557	0.27354339370604647	No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	535	0.2627391663065257	No Hit
GTACATGGGAATGGTATCAACGCAAAAAAAAAAAAAAAAAAAA	491	0.24113071150748439	No Hit
ACGCAGAGTACATGGGAAAAAAAAAAAAAAAAAAAAAAAAAAA	462	0.22688877538993438	No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTT	455	0.2234510666719051	No Hit
GGTATCAACGCAGAGTACATGGGAAAAAAAAAAAAAAAAAAAA	443	0.217557851726712	No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	413	0.20282481436372923	No Hit
CTCCGAGCCCACGAGACAGGCAGAAATCTCGTATGCCGTCTTC	394	0.19349389070050682	RNA PCR Primer, Index 17 (95% over 22bp)
TATCAACGCAGAGTACATGGGAAAAAAAAAAAAAAAAAAAAAA	383	0.18809177700074647	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTG	346	0.16992103091973443	No Hit
GTACATGGGAACAGTGGTATCAACGCAAAAAAAAAAAAAAAAA	332	0.1630456134836758	No Hit
GTACATGGGAAGCAGAGGTATCAACGCAAAAAAAAAAAAAAAA	323	0.15862570227478098	No Hit
TCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	318	0.15617019604761717	No Hit
CTCTATGGGCATATCAATAAGGCGAGGAATCACCGACTGCCCA	290	0.14241936117549994	No Hit
GAATGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAA	268	0.13161513377597925	No Hit
GTATCAACGCAGAGTACATGGGAAAAAAAAAAAAAAAAAAAAA	263	0.12915962754881546	No Hit
CACATCTCCGAGCCCACGAGACAGGCAGAAATCTCGTATGCCG	263	0.12915962754881546	No Hit
GTACATGGGAAGCAGTGGTATCAAAAAAAAAAAAAAAAAAAAA	255	0.1252308175853534	No Hit
CTATGGGCATATCAATAAGGCGAGGAATCACCGACTGCCCATA	244	0.11982870388559305	No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	241	0.11835540014929477	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGG	241	0.11835540014929477	No Hit
GTACATGGGCCTCTCTATGGGCATATCAATAAGGCGAGGAATC	231	0.11344438769496719	No Hit
CAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	225	0.11049778022237064	No Hit
GGGCATATCAATAAGGCGAGGAATCACCGACTGCCCATAGAGC	222	0.10902447648607237	No Hit
CTCTATGGGCAGTCGGTGATTCCTCCGCTTATTGATATGCCCA	221	0.1085333752406396	No Hit
CCCATAGAGAGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	219	0.1075511727497741	No Hit
ACGCAGAGTACATGGGCCTCTCTATGGGCATATCAATAAGGCG	219	0.1075511727497741	No Hit
CTCTATGGGCAGTCGGTGATTCCTCGCCTTATTGATATGCCCC	211	0.10362236278631201	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTC	207	0.10165795780458099	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.003437708718029309	0.0
3	0.0	0.0	0.0	0.004911012454327584	0.0
4	0.0	0.0	0.0	0.007857619926924134	0.0
5	0.0	0.0	0.0	0.008348721172356893	0.0
6	0.0	0.0	0.0	0.008348721172356893	0.0
7	0.0	0.0	0.0	0.00883982241778965	0.0
8	0.0	0.0	0.0	0.00933092366322241	0.0
9	0.0	0.0	0.0	0.014733037362982752	0.0
10	0.0	9.822024908655168E-4	0.0	0.020135151062743096	0.0
11	0.0	9.822024908655168E-4	0.0	0.022590657289906887	0.0
12	0.0	9.822024908655168E-4	0.0	0.02455506227163792	0.0
13	0.0	9.822024908655168E-4	0.0	0.026028366007936198	0.0
14	0.0	9.822024908655168E-4	0.0	0.02799277098966723	0.0
15	0.0	9.822024908655168E-4	0.0	0.031921580953129296	0.0
16	0.0	9.822024908655168E-4	0.0	0.04911012454327584	0.0
17	4.911012454327584E-4	9.822024908655168E-4	0.0	0.07022747809688445	0.0
18	4.911012454327584E-4	9.822024908655168E-4	0.0	0.0790673005146741	0.0
19	4.911012454327584E-4	9.822024908655168E-4	0.0	0.09772914784111893	0.0
20	4.911012454327584E-4	9.822024908655168E-4	0.0	0.10411346403174479	0.0
21	4.911012454327584E-4	9.822024908655168E-4	0.0	0.13357953875771028	0.0
22	4.911012454327584E-4	9.822024908655168E-4	0.0	0.1807252583192551	0.0
23	4.911012454327584E-4	9.822024908655168E-4	0.0	0.24751502769811023	0.0
24	4.911012454327584E-4	9.822024908655168E-4	0.0	0.36537932660197225	0.0
25	4.911012454327584E-4	9.822024908655168E-4	0.0	0.3914076926099085	0.0
26	4.911012454327584E-4	9.822024908655168E-4	0.0	0.4439555258712136	0.0
27	4.911012454327584E-4	9.822024908655168E-4	0.0	0.48569913173299806	0.0
28	4.911012454327584E-4	9.822024908655168E-4	0.0	0.5436490786940635	0.0
29	4.911012454327584E-4	9.822024908655168E-4	0.0	0.6453070364986445	0.0
30	4.911012454327584E-4	9.822024908655168E-4	0.0	0.8152280674183789	0.0
31	4.911012454327584E-4	9.822024908655168E-4	0.0	1.290614072997289	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CAAGTCG	20	0.001838381	37.0	29
GACAAGT	20	0.001838381	37.0	27
GGGTAAT	20	0.001838381	37.0	11
GTTCACG	20	0.001838381	37.0	6
TATTGAC	40	1.5915211E-6	32.375	30
CCGTCTT	265	0.0	32.11321	37
GCCGTCT	300	0.0	29.6	36
CTTGTTC	25	0.0054858583	29.599998	3
TGGGTAA	25	0.0054858583	29.599998	10
GGAAAAC	50	2.702218E-7	29.599998	8
AGTCGAG	25	0.0054858583	29.599998	31
ATTGACT	40	5.9165155E-5	27.750002	31
CGTCTTC	35	8.8442455E-4	26.428572	37
ATGGGTG	45	1.3179949E-4	24.666666	5
ATGCCCC	80	2.697925E-8	23.125002	37
ACAAGTC	40	0.0019257631	23.125002	28
TATACAC	410	0.0	23.012196	3
TGCCGTC	405	0.0	21.925926	35
CAGTGGC	60	3.7069363E-5	21.583334	26
ATGCCGT	405	0.0	21.469135	34
>>END_MODULE