FastQCFastQC Report
Fri 10 Feb 2017
ERR1630575.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1630575.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1428278
Sequences flagged as poor quality0
Sequence length43
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT82980.58097933315503No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT71760.5024231977248127No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT58100.4067835533418564No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT37260.2608735834340373No Hit
ACCTAGGCCTGGTCAGCATGTTGATGTATCTACGGAGATCAGC16030.1122330526690182No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGAACTA803.274181E-1127.75000224
CGCCTTA1800.024.66666625
ATACCGT1350.021.9259246
CGTCGTA1251.8189894E-1120.7210
GTCGGGT450.003826256620.55555524
ACCGTCG1303.274181E-1119.9230778
TACCGTC1500.019.7333347
GCAGTCG2750.019.509099
AGTCGGT2400.019.27083211
GACGGAC2050.018.9512187
TCGGGTA500.007035696418.525
CAGTCGG2600.018.510
CGGGTCG1451.546141E-1017.86206834
CGAAAGC2300.017.69565219
CTAGCAC1054.799276E-717.6190473
ACGGACC2250.017.2666668
GCGAAAG2200.016.81818218
CCGTCGT1655.4569682E-1116.8181829
CTCTATG5650.016.6991161
CTAGCGG1005.8797978E-616.65000229
Produced by FastQC (version 0.11.5)