##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1630575.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1428278
Sequences flagged as poor quality	0
Sequence length	43
%GC	48
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.02891873990918	33.0	31.0	34.0	30.0	34.0
2	32.18011969658568	33.0	31.0	34.0	30.0	34.0
3	32.28743353884888	34.0	31.0	34.0	30.0	34.0
4	35.87176376027636	37.0	35.0	37.0	35.0	37.0
5	35.851799859691184	37.0	35.0	37.0	35.0	37.0
6	35.89532429961114	37.0	35.0	37.0	35.0	37.0
7	35.86646787250101	37.0	35.0	37.0	35.0	37.0
8	35.86458658608478	37.0	35.0	37.0	35.0	37.0
9	37.59326755715624	39.0	37.0	39.0	35.0	39.0
10	37.494907854073226	39.0	37.0	39.0	35.0	39.0
11	37.6129584016557	39.0	37.0	39.0	35.0	39.0
12	37.541998126415166	39.0	37.0	39.0	35.0	39.0
13	37.594819075838174	39.0	37.0	39.0	35.0	39.0
14	38.86034231431136	40.0	38.0	41.0	35.0	41.0
15	38.83782638954041	40.0	38.0	41.0	35.0	41.0
16	38.81731637678379	40.0	38.0	41.0	35.0	41.0
17	38.81982709248479	40.0	38.0	41.0	35.0	41.0
18	38.81983689449813	40.0	38.0	41.0	35.0	41.0
19	38.84725312579204	40.0	38.0	41.0	35.0	41.0
20	38.834847977774636	40.0	38.0	41.0	35.0	41.0
21	38.76250491851026	40.0	38.0	41.0	35.0	41.0
22	38.7053276743043	40.0	38.0	41.0	34.0	41.0
23	38.68357980729242	40.0	38.0	41.0	34.0	41.0
24	38.63070844751512	40.0	38.0	41.0	34.0	41.0
25	38.58937125685616	40.0	38.0	41.0	34.0	41.0
26	38.40014969074648	40.0	38.0	41.0	34.0	41.0
27	38.28475618892121	40.0	38.0	41.0	34.0	41.0
28	38.183344558972415	40.0	38.0	41.0	33.0	41.0
29	38.078664657720694	40.0	37.0	41.0	33.0	41.0
30	37.98543490833017	40.0	37.0	41.0	33.0	41.0
31	37.91754966470113	40.0	37.0	41.0	33.0	41.0
32	37.81274863857036	40.0	37.0	41.0	33.0	41.0
33	37.76292010378932	40.0	37.0	41.0	33.0	41.0
34	37.672295589514086	40.0	37.0	41.0	33.0	41.0
35	37.5995702517297	40.0	37.0	41.0	33.0	41.0
36	37.53000956396444	40.0	37.0	41.0	33.0	41.0
37	37.33605572584609	40.0	36.0	41.0	32.0	41.0
38	37.20987230777202	40.0	36.0	41.0	31.0	41.0
39	37.126602804215985	40.0	36.0	41.0	31.0	41.0
40	36.99560239673229	40.0	36.0	41.0	31.0	41.0
41	36.852159733609284	39.0	35.0	41.0	31.0	41.0
42	36.74196339928221	39.0	35.0	41.0	30.0	41.0
43	35.88443356265377	38.0	35.0	40.0	28.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
9	2.0
10	10.0
11	4.0
12	11.0
13	4.0
14	6.0
15	6.0
16	5.0
17	15.0
18	48.0
19	72.0
20	170.0
21	329.0
22	638.0
23	1100.0
24	1986.0
25	3079.0
26	5021.0
27	7510.0
28	10724.0
29	15387.0
30	20791.0
31	27133.0
32	34953.0
33	45226.0
34	60317.0
35	81213.0
36	113347.0
37	178189.0
38	313949.0
39	507033.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	44.43812759140727	18.950792492778014	12.128661227016028	24.48241868879868
2	18.68865865048681	20.92267751796219	34.31089745833794	26.077766373213056
3	20.628407074813165	23.267669179249417	29.347718021281572	26.756205724655846
4	14.259058810679715	15.14712121869832	34.05198427757061	36.541835693051354
5	14.313810056585622	37.45202264545138	33.805533656613065	14.428633641349933
6	35.22766576254763	34.15644573395375	15.586811531088484	15.029076972410133
7	29.209859705183444	30.586412449117052	21.200284538444198	19.00344330725531
8	28.548993963360076	32.23574122124685	19.7920152799385	19.42324953545458
9	26.85625627503889	13.352442591708336	20.13207512823134	39.65922600502143
10	17.272267723790467	27.192955433045945	32.52791123296725	23.006865610196332
11	35.17305454540362	21.952098961126616	21.319589043589556	21.555257449880205
12	22.117262885796745	25.909101729495237	28.79201387965088	23.18162150505714
13	30.53516192225883	19.677681795840865	25.43335401091384	24.35380227098646
14	22.9325803520043	21.020347579392805	25.95314077511521	30.09393129348768
15	25.84146783749382	27.673674172675067	22.80410396295399	23.68075402687712
16	24.752604184899578	26.366085593981005	24.774308642995273	24.107001578124144
17	23.2792215521068	26.58565069265227	26.15772279626235	23.977404958978575
18	23.037601923435076	24.976510175189983	28.00666256849157	23.979225332883374
19	24.131716654600854	25.368100607864857	27.884837545631875	22.615345191902417
20	24.718927267660778	24.707024822898624	27.19799646847462	23.376051440965977
21	24.925819763379398	24.88871214147386	26.90680665808757	23.278661437059174
22	24.66326583480247	25.183752742813375	26.97374040627945	23.179241016104708
23	23.886456278119525	25.172690470622666	26.885382257515694	24.055470993742116
24	23.554378069255424	25.68470563853816	27.359799702858968	23.401116589347453
25	23.92132343983454	25.502878291201014	26.851565311514985	23.72423295744946
26	23.680053883067583	26.00194079864004	26.9701696728508	23.347835645441574
27	23.86034091402374	25.391695454246303	26.869979093705847	23.877984538024112
28	23.254646504392003	25.86408248254191	27.144715524568745	23.736555488497338
29	23.19352394981929	26.228787392930506	27.578244571434972	22.999444085815227
30	23.341884423060495	26.28269846626497	27.051876455423944	23.32354065525059
31	22.996853553719934	26.63599103255809	27.02576109132816	23.341394322393818
32	22.700062592856575	26.046119873021915	27.145975783425914	24.107841750695595
33	22.431207369993796	26.25105196607383	27.513481269052665	23.804259394879708
34	23.78059453411731	25.42222172434218	27.119020246758684	23.678163494781828
35	23.116228073246244	25.613711056250953	27.515021585433647	23.755039285069156
36	22.431767485041426	26.079236675213092	27.591547303816206	23.897448535929282
37	23.054615418006858	25.0969349104306	28.02584650887292	23.822603162689614
38	22.79374183457282	25.181792340146664	28.041459715825628	23.983006109454884
39	22.68689988923725	25.051565591572512	28.276077906401976	23.985456612788266
40	22.0310051684616	24.909226355093335	28.90935798212953	24.150410494315533
41	21.23571181520684	25.25348706624341	29.287225596137446	24.223575522412304
42	21.824882830933472	24.99555408680943	29.411011021663853	23.768552060593244
43	21.613649443595715	24.859516144616105	29.168621234801627	24.358213176986553
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	304.0
1	277.5
2	251.0
3	759.5
4	1268.0
5	1268.0
6	1824.5
7	2381.0
8	2338.5
9	2296.0
10	3280.5
11	4265.0
12	4265.0
13	7214.5
14	10164.0
15	14762.5
16	19361.0
17	18138.0
18	16915.0
19	16915.0
20	19627.0
21	22339.0
22	20675.5
23	19012.0
24	21277.0
25	23542.0
26	23542.0
27	26206.5
28	28871.0
29	32482.0
30	36093.0
31	39926.5
32	43760.0
33	43760.0
34	48129.5
35	52499.0
36	56919.0
37	61339.0
38	65638.5
39	69938.0
40	69938.0
41	71945.0
42	73952.0
43	75574.5
44	77197.0
45	79374.0
46	81551.0
47	81551.0
48	85370.5
49	89190.0
50	89853.5
51	90517.0
52	92033.5
53	93550.0
54	93550.0
55	90914.0
56	88278.0
57	86942.5
58	85607.0
59	84116.0
60	82625.0
61	82625.0
62	76486.5
63	70348.0
64	63825.0
65	57302.0
66	49769.0
67	42236.0
68	42236.0
69	37548.5
70	32861.0
71	27501.0
72	22141.0
73	16965.5
74	11790.0
75	11790.0
76	8833.0
77	5876.0
78	4760.5
79	3645.0
80	2929.0
81	2213.0
82	2213.0
83	1678.0
84	1143.0
85	964.5
86	786.0
87	667.0
88	548.0
89	548.0
90	411.5
91	275.0
92	155.0
93	35.0
94	22.5
95	10.0
96	10.0
97	5.0
98	0.0
99	2.0
100	4.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	1428278.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	62.93803386380517
#Duplication Level	Percentage of deduplicated	Percentage of total
1	87.54624215703211	55.099883535281734
2	7.948277187632902	10.004978775882991
3	1.8940854607421151	3.576300446073847
4	0.7272313249830311	1.8308203903440765
5	0.40569119603066317	1.2766703117012748
6	0.25024675174447314	0.9450023121360537
7	0.17831469307066716	0.785594333362697
8	0.13018783968030873	0.6555013329963927
9	0.09610845597815453	0.5443989531256005
>10	0.6517120527792402	8.066164557139667
>50	0.08783819355736686	3.90635912941885
>100	0.07927693458309432	9.484076147013825
>500	0.0036743214449632996	1.562019487949973
>1k	7.794015186285788E-4	0.7707084041486676
>5k	3.340292222693909E-4	1.491521883424281
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	8298	0.58097933315503	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	7176	0.5024231977248127	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	5810	0.4067835533418564	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	3726	0.2608735834340373	No Hit
ACCTAGGCCTGGTCAGCATGTTGATGTATCTACGGAGATCAGC	1603	0.1122330526690182	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	7.001438095384792E-5	0.0	2.1004314286154376E-4	0.0
3	0.0	7.001438095384792E-5	0.0	4.901006666769355E-4	0.0
4	0.0	7.001438095384792E-5	0.0	7.701581904923272E-4	0.0
5	0.0	7.001438095384792E-5	0.0	8.40172571446175E-4	0.0
6	0.0	7.001438095384792E-5	0.0	8.40172571446175E-4	0.0
7	0.0	7.001438095384792E-5	0.0	9.10186952400023E-4	0.0
8	0.0	7.001438095384792E-5	0.0	0.0010502157143077188	0.0
9	0.0	7.001438095384792E-5	0.0	0.0021704458095692853	0.0
10	0.0	7.001438095384792E-5	0.0	0.003570733428646244	0.0
11	0.0	7.001438095384792E-5	0.0	0.004060834095323179	0.0
12	0.0	7.001438095384792E-5	0.0	0.004760977904861658	0.0
13	0.0	7.001438095384792E-5	0.0	0.005041035428677051	0.0
14	0.0	7.001438095384792E-5	0.0	0.006021236762030921	0.0
15	0.0	7.001438095384792E-5	0.0	0.007771596285877119	0.0
16	0.0	7.001438095384792E-5	0.0	0.01141234409547721	0.0
17	0.0	7.001438095384792E-5	0.0	0.016173322000338868	0.0
18	0.0	7.001438095384792E-5	0.0	0.017643624000369677	0.0
19	0.0	7.001438095384792E-5	0.0	0.02121435742901592	0.0
20	0.0	7.001438095384792E-5	0.0	0.023734875143354445	0.0
21	0.0	7.001438095384792E-5	0.0	0.02961608314347767	0.0
22	0.0	7.001438095384792E-5	0.0	0.03927806771510868	0.0
23	0.0	7.001438095384792E-5	0.0	0.05671164857261682	0.0
24	0.0	7.001438095384792E-5	0.0	0.08450735781129444	0.0
25	0.0	7.001438095384792E-5	0.0	0.09157881028763308	0.0
26	0.0	7.001438095384792E-5	0.0	0.10131080924021794	0.0
27	0.0	7.001438095384792E-5	0.0	0.11909446200249531	0.0
28	0.0	7.001438095384792E-5	0.0	0.16096306181289638	0.0
29	0.0	7.001438095384792E-5	0.0	0.2238359759094518	0.0
30	0.0	7.001438095384792E-5	0.0	0.32101593667339273	0.0
31	0.0	7.001438095384792E-5	0.0	0.6475630094421394	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGAACTA	80	3.274181E-11	27.750002	24
CGCCTTA	180	0.0	24.666666	25
ATACCGT	135	0.0	21.925924	6
CGTCGTA	125	1.8189894E-11	20.72	10
GTCGGGT	45	0.0038262566	20.555555	24
ACCGTCG	130	3.274181E-11	19.923077	8
TACCGTC	150	0.0	19.733334	7
GCAGTCG	275	0.0	19.50909	9
AGTCGGT	240	0.0	19.270832	11
GACGGAC	205	0.0	18.951218	7
TCGGGTA	50	0.0070356964	18.5	25
CAGTCGG	260	0.0	18.5	10
CGGGTCG	145	1.546141E-10	17.862068	34
CGAAAGC	230	0.0	17.695652	19
CTAGCAC	105	4.799276E-7	17.619047	3
ACGGACC	225	0.0	17.266666	8
GCGAAAG	220	0.0	16.818182	18
CCGTCGT	165	5.4569682E-11	16.818182	9
CTCTATG	565	0.0	16.699116	1
CTAGCGG	100	5.8797978E-6	16.650002	29
>>END_MODULE