FastQCFastQC Report
Fri 10 Feb 2017
ERR1630574.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1630574.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences392303
Sequences flagged as poor quality0
Sequence length43
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT43511.1090916969791207No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT35540.9059324042895415No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT27140.6918121961850916No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT19210.48967252353410495No Hit
CTTATACACATCTCCGAGCCCACGAGACCGTACTAGATCTCGT8900.2268654585868576No Hit
TCTCCGAGCCCACGAGACCGTACTAGATCTCGTATGCCGTCTT7970.20315929268957925TruSeq Adapter, Index 11 (95% over 21bp)
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTT4840.12337402466970683No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT4790.12209949962146607No Hit
AACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT4770.12158968960216976No Hit
ATTGAAAGCTGAGTATTTTTAAGACAAAGGTTTCAGGAAGAAA4690.1195504495249845No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT4260.10858953411011385No Hit
ATACACATCTCCGAGCCCACGAGACCGTACTAGATCTCGTATG4180.10655029403292864No Hit
ACACATCTCCGAGCCCACGAGACCGTACTAGATCTCGTATGCC4020.10247181387855815No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACACTA451.3210425E-424.6666685
CGCTTAT451.3210425E-424.66666826
CTCCGCT502.697708E-422.223
CCGCTTA502.697708E-422.225
CATACAC656.887327E-519.9230773
CTCTAGA851.2410346E-619.5882341
TCCGCTT609.222041E-418.524
TACACTT500.00702682618.55
GGTATCA19050.017.965881
GCCCCTA650.001577426917.07692327
GTGTGCA650.001577426917.0769239
GATATGC2000.016.65000234
TATACAG1005.857961E-616.6500025
TACACCA1005.857961E-616.6500025
AGCAAGC1352.2062522E-816.44444527
AGCCCTC1352.2062522E-816.44444531
GGGCAGT2600.016.3653857
AGTCGGT2600.016.36538511
CCCCTAC803.3754663E-416.187528
GATAATC803.3754663E-416.187517
Produced by FastQC (version 0.11.5)