FastQCFastQC Report
Fri 10 Feb 2017
ERR1630573.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1630573.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2693100
Sequences flagged as poor quality0
Sequence length43
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT72500.26920649066131963No Hit
CTCCAGGACAGGCTGCATCAGAAGAGGCCATCAAGCAGATCAC66640.24744717982993578No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT66580.24722438825145743No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT57050.2118376592031488No Hit
GTGCAGGAGGCGGCGGGTGGGGGGCTGCCTGCGGGCTGCGTCT40720.15120121792729568No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT40300.14964167687794735No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGTCG650.001580679417.0769235
TGCATCA18950.016.59630614
CCAGGAC22750.016.5076943
GACAGGC20400.016.323537
GGTATCA35200.016.18751
AAGACGG6100.016.073775
GCATCAG19800.015.97727215
ACAGGCT20800.015.9206738
GACGGAC6100.015.7704937
GCTGCAT20100.015.64676612
ATCAAGC20150.015.516129530
ACGGACC6250.015.3920018
CAGGACA27000.015.1425924
CGCAAGA6650.015.0225562
GCGCAAG7150.015.0069921
CTTATAC16950.014.84365837
GGCCATC21750.014.71494326
TACACTT3400.014.6911765
CTGCATC21800.014.5963313
CGAACGA3050.014.55737716
Produced by FastQC (version 0.11.5)