##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1630573.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	2693100
Sequences flagged as poor quality	0
Sequence length	43
%GC	50
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.086956295718686	33.0	31.0	34.0	30.0	34.0
2	32.25771415840481	34.0	31.0	34.0	30.0	34.0
3	32.363138019382866	34.0	31.0	34.0	30.0	34.0
4	35.939473097916895	37.0	35.0	37.0	35.0	37.0
5	35.91359251420296	37.0	35.0	37.0	35.0	37.0
6	35.9460320819873	37.0	35.0	37.0	35.0	37.0
7	35.920392855816715	37.0	35.0	37.0	35.0	37.0
8	35.91255690468233	37.0	35.0	37.0	35.0	37.0
9	37.66172960528759	39.0	37.0	39.0	35.0	39.0
10	37.568499870038245	39.0	37.0	39.0	35.0	39.0
11	37.66889309717426	39.0	37.0	39.0	35.0	39.0
12	37.59950317477999	39.0	37.0	39.0	35.0	39.0
13	37.63937952545394	39.0	37.0	39.0	35.0	39.0
14	38.95058148601983	40.0	38.0	41.0	36.0	41.0
15	38.924043667149384	40.0	38.0	41.0	36.0	41.0
16	38.912430656121195	40.0	38.0	41.0	35.0	41.0
17	38.895345141286995	40.0	38.0	41.0	35.0	41.0
18	38.85445583156957	40.0	38.0	41.0	35.0	41.0
19	38.874867253351155	40.0	38.0	41.0	35.0	41.0
20	38.86066391890387	40.0	38.0	41.0	35.0	41.0
21	38.793785228918345	40.0	38.0	41.0	35.0	41.0
22	38.73978723404255	40.0	38.0	41.0	35.0	41.0
23	38.7198098845197	40.0	38.0	41.0	35.0	41.0
24	38.6722847276373	40.0	38.0	41.0	34.0	41.0
25	38.62228992610746	40.0	38.0	41.0	34.0	41.0
26	38.44478630574431	40.0	38.0	41.0	34.0	41.0
27	38.348587501392444	40.0	38.0	41.0	34.0	41.0
28	38.24807916527422	40.0	38.0	41.0	34.0	41.0
29	38.15263191118042	40.0	38.0	41.0	33.0	41.0
30	38.08026289406261	40.0	38.0	41.0	33.0	41.0
31	38.05227247410048	40.0	37.0	41.0	33.0	41.0
32	37.95941777134158	40.0	37.0	41.0	33.0	41.0
33	37.92814637406706	40.0	37.0	41.0	33.0	41.0
34	37.850526902083104	40.0	37.0	41.0	33.0	41.0
35	37.78971111358657	40.0	37.0	41.0	33.0	41.0
36	37.74741338977387	40.0	37.0	41.0	33.0	41.0
37	37.57136608369537	40.0	37.0	41.0	32.0	41.0
38	37.47116557127474	40.0	37.0	41.0	32.0	41.0
39	37.417650662804945	40.0	36.0	41.0	32.0	41.0
40	37.311491589617916	40.0	36.0	41.0	32.0	41.0
41	37.182057851546546	40.0	36.0	41.0	31.0	41.0
42	37.099407374401245	39.0	36.0	41.0	31.0	41.0
43	36.239154505959675	38.0	35.0	40.0	30.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	3.0
9	2.0
10	6.0
11	4.0
12	4.0
13	4.0
14	2.0
15	4.0
16	15.0
17	30.0
18	66.0
19	145.0
20	298.0
21	614.0
22	1103.0
23	1963.0
24	3253.0
25	5248.0
26	8247.0
27	12235.0
28	17448.0
29	24990.0
30	34377.0
31	45904.0
32	61105.0
33	80390.0
34	110963.0
35	152185.0
36	211855.0
37	327682.0
38	601798.0
39	991157.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	44.20764175114181	18.157922097211394	11.401916007574913	26.232520144071884
2	19.320040102484125	19.729122572500092	34.53896996026884	26.411867364746943
3	20.711707697449036	22.789759013775946	28.159147450893023	28.339385837882
4	14.70168207641751	14.640005941108761	33.479744532323345	37.17856745015038
5	15.00906019085812	36.543797111135866	33.49240652036686	14.954736177639152
6	36.587389996658125	33.47283799339051	15.138910549181242	14.800861460770115
7	30.377223274293563	29.265567561546174	21.175596895770674	19.18161226838959
8	28.045932197096285	32.84230069436709	19.29790947235528	19.813857636181353
9	27.362221974676025	13.221157773569491	19.054472540937955	40.36214771081653
10	17.96134566113401	25.768111098733804	31.819687349151536	24.450855890980655
11	36.89086925847536	21.055994950057556	20.901934573539787	21.151201217927294
12	23.264973450670233	24.38531803497828	27.96802940848836	24.38167910586313
13	30.123315138687758	19.074152463703538	24.923359697003452	25.879172700605253
14	23.32145111581449	19.98633544985333	24.76413798225094	31.928075452081245
15	26.40730013738814	26.620177490624187	22.023764435037688	24.948757936949985
16	26.213731387620214	25.17604247892763	23.116111544316958	25.494114589135197
17	24.448627975195873	25.827410790538785	24.820875570903418	24.903085663361928
18	24.39916824477368	24.018677360662434	26.32453306598344	25.25762132858045
19	25.117336897998587	24.28372507519216	25.675949649103263	24.92298837770599
20	25.005198470164498	24.314210389513942	25.82403178493186	24.8565593553897
21	26.57209164160261	24.19134083398314	25.028183134677512	24.208384389736732
22	25.9614199249935	24.351193791541345	25.006163900337903	24.681222383127253
23	24.98860049756786	24.54023244588021	25.376592031487878	25.094575025064053
24	25.302922282871045	24.906761724406817	24.862686123797854	24.92762986892429
25	25.323344844231556	24.25257138613494	25.47851917864171	24.945564590991793
26	25.310497196539306	25.02848019011548	25.588318294901786	24.07270431844343
27	25.99164531580706	24.334892874382682	24.64520441127325	25.028257398537
28	24.518510266978573	24.180312650848464	26.26333964576139	25.03783743641157
29	24.682596264527866	24.48082135828599	26.237421558798413	24.59916081838773
30	24.211020756748727	24.951245776242992	26.068174223014367	24.76955924399391
31	24.57450521703613	25.02354164345921	25.334410159295977	25.067542980208678
32	24.110690282573984	24.578923916675947	25.621254316586832	25.68913148416323
33	24.569826593888084	24.60855519661357	25.696892057480227	25.124726152018123
34	25.362816085551966	24.578032750362038	25.618729345364077	24.44042181872192
35	25.196019457131186	24.47733095688983	26.04544948200958	24.281200103969404
36	24.574319557387398	25.01511269540678	25.008317552263193	25.40225019494263
37	24.39660614162118	24.094723552783037	26.06665181389477	25.442018491701013
38	24.498681816494003	23.8236604656344	26.532917455720174	25.144740262151423
39	23.923248301214215	24.108945081875905	26.468085106382976	25.4997215105269
40	24.155805577215848	23.807322416545986	27.176228138576363	24.860643867661803
41	23.0354609929078	23.87906130481601	27.80123278006758	25.28424492220861
42	23.469013404626637	24.38806579777951	27.242137313876203	24.900783483717646
43	23.319371727748692	22.874828264824924	27.82187813300657	25.983921874419813
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	560.0
1	461.0
2	362.0
3	925.0
4	1488.0
5	1488.0
6	1988.5
7	2489.0
8	2390.5
9	2292.0
10	3316.0
11	4340.0
12	4340.0
13	7638.5
14	10937.0
15	14327.0
16	17717.0
17	17186.5
18	16656.0
19	16656.0
20	19578.5
21	22501.0
22	21271.5
23	20042.0
24	22978.0
25	25914.0
26	25914.0
27	30465.5
28	35017.0
29	41129.5
30	47242.0
31	55200.5
32	63159.0
33	63159.0
34	75364.0
35	87569.0
36	98598.5
37	109628.0
38	123003.5
39	136379.0
40	136379.0
41	143929.5
42	151480.0
43	157052.0
44	162624.0
45	164514.0
46	166404.0
47	166404.0
48	171310.0
49	176216.0
50	181186.0
51	186156.0
52	190631.0
53	195106.0
54	195106.0
55	193812.0
56	192518.0
57	182499.5
58	172481.0
59	161710.0
60	150939.0
61	150939.0
62	143817.0
63	136695.0
64	121640.0
65	106585.0
66	92924.5
67	79264.0
68	79264.0
69	69130.5
70	58997.0
71	52855.0
72	46713.0
73	44179.0
74	41645.0
75	41645.0
76	37524.5
77	33404.0
78	25791.5
79	18179.0
80	12049.0
81	5919.0
82	5919.0
83	4331.0
84	2743.0
85	2439.0
86	2135.0
87	1892.5
88	1650.0
89	1650.0
90	1239.5
91	829.0
92	460.0
93	91.0
94	59.5
95	28.0
96	28.0
97	16.0
98	4.0
99	3.5
100	3.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	2693100.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	48.37270649610662
#Duplication Level	Percentage of deduplicated	Percentage of total
1	82.82199672904042	40.063241391953746
2	9.692263999576209	9.37682083468561
3	2.904788370751762	4.215374258750362
4	1.2864351488709787	2.489133995304443
5	0.709521262552234	1.716073189309311
6	0.4596759081474398	1.334146067288842
7	0.3143123068988039	1.064289587881102
8	0.23143709729699324	0.8956191023886663
9	0.17435939275117568	0.7590812157352802
>10	1.1233966525910106	10.48633553941466
>50	0.13642844042639385	4.614676862798969
>100	0.12330762312508343	12.585565950272896
>500	0.016153952174279743	5.344809838966913
>1k	0.005615421470106283	4.077064510653288
>5k	3.076943271291114E-4	0.977767654596067
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	7250	0.26920649066131963	No Hit
CTCCAGGACAGGCTGCATCAGAAGAGGCCATCAAGCAGATCAC	6664	0.24744717982993578	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	6658	0.24722438825145743	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	5705	0.2118376592031488	No Hit
GTGCAGGAGGCGGCGGGTGGGGGGCTGCCTGCGGGCTGCGTCT	4072	0.15120121792729568	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	4030	0.14964167687794735	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	7.426385949277784E-5	7.426385949277784E-5
2	0.0	0.0	0.0	4.8271508670305596E-4	7.426385949277784E-5
3	0.0	0.0	0.0	9.654301734061119E-4	7.426385949277784E-5
4	0.0	0.0	0.0	0.001893728417065835	7.426385949277784E-5
5	0.0	0.0	0.0	0.0020793880657977797	7.426385949277784E-5
6	0.0	0.0	0.0	0.002153651925290557	7.426385949277784E-5
7	0.0	0.0	0.0	0.0025249712227544464	7.426385949277784E-5
8	0.0	0.0	0.0	0.002822026660725558	1.1139578923916676E-4
9	7.426385949277784E-5	0.0	0.0	0.004530095429059448	1.1139578923916676E-4
10	7.426385949277784E-5	0.0	0.0	0.006275296127139727	1.1139578923916676E-4
11	7.426385949277784E-5	0.0	0.0	0.006980802792321117	1.1139578923916676E-4
12	7.426385949277784E-5	0.0	0.0	0.007686309457502506	1.1139578923916676E-4
13	7.426385949277784E-5	0.0	0.0	0.008391816122683896	1.4852771898555569E-4
14	7.426385949277784E-5	0.0	0.0	0.009097322787865285	1.4852771898555569E-4
15	1.1139578923916676E-4	0.0	0.0	0.010359808399242509	1.4852771898555569E-4
16	1.1139578923916676E-4	0.0	0.0	0.011919349448590843	1.856596487319446E-4
17	1.1139578923916676E-4	0.0	0.0	0.014555716460584456	1.856596487319446E-4
18	1.1139578923916676E-4	0.0	0.0	0.015409750844751402	2.2279157847833353E-4
19	1.1139578923916676E-4	0.0	0.0	0.017229215402324458	2.2279157847833353E-4
20	1.1139578923916676E-4	0.0	0.0	0.019011548030151128	2.2279157847833353E-4
21	1.1139578923916676E-4	0.0	0.0	0.02194497048011585	2.5992350822472246E-4
22	1.1139578923916676E-4	0.0	0.0	0.026326538190189742	2.5992350822472246E-4
23	1.1139578923916676E-4	0.0	0.0	0.0335301325609892	2.5992350822472246E-4
24	1.4852771898555569E-4	0.0	0.0	0.04452118376592031	2.5992350822472246E-4
25	1.4852771898555569E-4	0.0	0.0	0.04786305744309532	2.5992350822472246E-4
26	1.4852771898555569E-4	0.0	0.0	0.055846422338568937	2.9705543797111137E-4
27	1.4852771898555569E-4	0.0	0.0	0.07188741598900895	3.341873677175003E-4
28	1.4852771898555569E-4	0.0	0.0	0.1119527681853626	3.341873677175003E-4
29	1.4852771898555569E-4	0.0	0.0	0.16646244105306152	3.341873677175003E-4
30	1.4852771898555569E-4	0.0	0.0	0.2476699714084141	3.341873677175003E-4
31	1.4852771898555569E-4	0.0	0.0	0.5098585273476662	3.341873677175003E-4
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TATGTCG	65	0.0015806794	17.076923	5
TGCATCA	1895	0.0	16.596306	14
CCAGGAC	2275	0.0	16.507694	3
GACAGGC	2040	0.0	16.32353	7
GGTATCA	3520	0.0	16.1875	1
AAGACGG	610	0.0	16.07377	5
GCATCAG	1980	0.0	15.977272	15
ACAGGCT	2080	0.0	15.920673	8
GACGGAC	610	0.0	15.770493	7
GCTGCAT	2010	0.0	15.646766	12
ATCAAGC	2015	0.0	15.5161295	30
ACGGACC	625	0.0	15.392001	8
CAGGACA	2700	0.0	15.142592	4
CGCAAGA	665	0.0	15.022556	2
GCGCAAG	715	0.0	15.006992	1
CTTATAC	1695	0.0	14.843658	37
GGCCATC	2175	0.0	14.714943	26
TACACTT	340	0.0	14.691176	5
CTGCATC	2180	0.0	14.59633	13
CGAACGA	305	0.0	14.557377	16
>>END_MODULE