FastQCFastQC Report
Fri 10 Feb 2017
ERR1630572.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1630572.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1649765
Sequences flagged as poor quality0
Sequence length43
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT27270.1652962694686819No Hit
GTGTAGCGCGCGTGCAGCCCCGGACATCTAAGGGCATCACAGA23500.1424445299785121No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT22780.1380802720387449No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAATGTTTT21770.13195818798434927No Hit
GGGTAGGCACACGCTGAGCCAGTCAGTGTAGCGCGCGTGCAGC21220.12862437983591604No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT19130.11595590887186963No Hit
GTTCAAAGCAGGCCCGAGCCGCCTGGATACCGCAGCTAGGAAT17620.10680309013708013No Hit
TGCCTACCCTACGCCGGCAGGCGCGGGTAACCCGTTGAACCCC17550.10637878728182498No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGACGG4250.021.7647065
GCGTTAC450.003826503920.55555514
GGTATCA18950.020.2084431
CAAGACG4950.019.8080794
GTCTAGG1800.018.51
TCGACAC609.239142E-418.53
CGCGAAC500.0070361518.49999828
CGCAAGA4800.018.1145822
GACGGAC5150.017.6019427
CGAAATA650.001580328217.07692324
GCGCAAG5350.016.9439241
CGCAATA4500.016.85555536
CGGACCA5100.016.6862749
GCTGCGT904.4478445E-516.4444458
TCTAGCG4500.016.44444328
ACCGTTT700.002593134815.85714319
ACGGACC5500.015.8090928
CGGTCCA5450.015.61467810
GTCCTAT4500.015.211111
AGACGGA6100.015.1639356
Produced by FastQC (version 0.11.5)