FastQCFastQC Report
Fri 10 Feb 2017
ERR1630571.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1630571.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2289205
Sequences flagged as poor quality0
Sequence length43
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT108420.473614202310409No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT102210.44648688081670274No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT81390.3555382763885279No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT57990.2533193838035475No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGACGG4150.018.277115
ACGGACC3950.017.7974688
CGCAAGA4250.016.976472
CAAGACG4700.016.5319164
CGGACCA4300.016.3488379
GACGGAC4150.016.0481937
TCTAGCG2250.015.62222228
CTACGGT855.367038E-415.2352944
GCGCAAG4700.014.9574471
GGTATCA58650.014.5098041
AGACGGA4900.014.3469386
CTTATAC18500.014.337
TATACTG2201.8189894E-1114.2954545
AACCGCG1956.730261E-1014.230777
CTATACA1956.730261E-1014.230774
GCGAAAG4700.014.17021418
TAATACT3450.013.9420284
CGCAATA2405.456968E-1213.87499936
GAGCGAA4950.013.82828316
TAGGACC6900.013.6739124
Produced by FastQC (version 0.11.5)