##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1630571.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	2289205
Sequences flagged as poor quality	0
Sequence length	43
%GC	47
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.023093606732466	33.0	31.0	34.0	30.0	34.0
2	32.17943172411383	33.0	31.0	34.0	30.0	34.0
3	32.27736091787324	34.0	31.0	34.0	30.0	34.0
4	35.861710069653	37.0	35.0	37.0	35.0	37.0
5	35.84055643771528	37.0	35.0	37.0	35.0	37.0
6	35.88138851697423	37.0	35.0	37.0	35.0	37.0
7	35.85258375724324	37.0	35.0	37.0	35.0	37.0
8	35.85051841141357	37.0	35.0	37.0	35.0	37.0
9	37.582450239275204	39.0	37.0	39.0	35.0	39.0
10	37.48722635150631	39.0	37.0	39.0	35.0	39.0
11	37.59888389200618	39.0	37.0	39.0	35.0	39.0
12	37.53340045998502	39.0	37.0	39.0	35.0	39.0
13	37.579679845186426	39.0	37.0	39.0	35.0	39.0
14	38.85691888668774	40.0	38.0	41.0	35.0	41.0
15	38.83433200608945	40.0	38.0	41.0	35.0	41.0
16	38.80277825708051	40.0	38.0	41.0	35.0	41.0
17	38.80250611019983	40.0	38.0	41.0	35.0	41.0
18	38.7907535585498	40.0	38.0	41.0	35.0	41.0
19	38.83461987895361	40.0	38.0	41.0	35.0	41.0
20	38.81732173396441	40.0	38.0	41.0	35.0	41.0
21	38.74084496582875	40.0	38.0	41.0	34.0	41.0
22	38.69002645023054	40.0	38.0	41.0	34.0	41.0
23	38.658651365867186	40.0	38.0	41.0	34.0	41.0
24	38.60531101408568	40.0	38.0	41.0	34.0	41.0
25	38.56435880578629	40.0	38.0	41.0	34.0	41.0
26	38.37043864573072	40.0	38.0	41.0	34.0	41.0
27	38.253297978992705	40.0	38.0	41.0	34.0	41.0
28	38.15938415301382	40.0	38.0	41.0	33.0	41.0
29	38.05264840850863	40.0	37.0	41.0	33.0	41.0
30	37.96348120854183	40.0	37.0	41.0	33.0	41.0
31	37.89703761786297	40.0	37.0	41.0	33.0	41.0
32	37.79156126253437	40.0	37.0	41.0	33.0	41.0
33	37.75090304275938	40.0	37.0	41.0	33.0	41.0
34	37.661255763463735	40.0	37.0	41.0	33.0	41.0
35	37.59093921252138	40.0	37.0	41.0	33.0	41.0
36	37.533660375545224	40.0	37.0	41.0	33.0	41.0
37	37.33161162936478	40.0	36.0	41.0	32.0	41.0
38	37.20494101664115	40.0	36.0	41.0	31.0	41.0
39	37.11876917969339	40.0	36.0	41.0	31.0	41.0
40	37.004376191734686	40.0	36.0	41.0	31.0	41.0
41	36.85792666012873	39.0	35.0	41.0	31.0	41.0
42	36.75123896723972	39.0	35.0	41.0	30.0	41.0
43	35.91143912406272	38.0	35.0	40.0	28.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
9	3.0
10	15.0
11	9.0
12	6.0
13	6.0
14	2.0
15	2.0
16	11.0
17	34.0
18	95.0
19	176.0
20	315.0
21	684.0
22	1186.0
23	2077.0
24	3461.0
25	5519.0
26	8307.0
27	12192.0
28	17869.0
29	25017.0
30	33780.0
31	43560.0
32	56811.0
33	73348.0
34	96524.0
35	128658.0
36	180438.0
37	279192.0
38	497275.0
39	822633.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	43.790398850255876	19.28582193381545	12.336640886246537	24.587138329682137
2	18.425304854742148	21.370825242824473	35.15290242682504	25.050967475608342
3	20.557966630336733	23.808702147688827	29.460795341614226	26.172535880360215
4	14.474850439344664	15.721396729432271	34.00564824906463	35.79810458215844
5	14.08274925137766	37.60327275189422	34.00224095264513	14.311737044082989
6	34.60341035424962	34.24909520990912	16.024515060905422	15.122979374935841
7	28.470626265450232	30.88028376663514	21.765241645025238	18.88384832288939
8	27.00570722150266	33.57283423721336	20.33526923102125	19.086189310262732
9	26.51544968668162	13.905919303863131	20.403633575848385	39.17499743360686
10	17.0515528316599	27.251338346718622	33.01421235756518	22.6828964640563
11	34.500143062766334	22.26301270528415	21.750433010586644	21.486411221362875
12	21.162630694935576	26.255708859625944	29.37679237988734	23.20486806555114
13	29.40125502084785	20.261837624852298	26.197173254470442	24.139734099829415
14	22.711858483622045	21.617286350501594	25.9603224700278	29.710532695848556
15	25.53912821263277	27.75583663324167	23.39261883492304	23.312416319202516
16	24.66664191280379	26.812496041202078	25.027291133821567	23.49357091217257
17	23.007725389381903	27.15095415220568	26.344254883245494	23.497065575166925
18	22.466926291004956	25.534672517314966	28.195465237931945	23.802935953748136
19	23.485183720986104	26.005272572792737	28.0735888660037	22.435954840217455
20	23.908212676453182	25.070930737963614	28.134570735255256	22.886285850327955
21	24.084736840955703	25.7503369073543	27.11150814365686	23.05341810803314
22	24.017158795302297	25.572982760390616	26.9678338113013	23.442024633005783
23	23.234965850590054	25.896501187093335	27.371642120299406	23.49689084201721
24	23.492828296286266	26.34106600326314	27.220366895931118	22.945738804519472
25	23.708099536738736	25.731902560059062	27.22975880272846	23.330239100473747
26	23.335874244552148	26.38750133780068	27.309655535436978	22.9669688822102
27	23.741779351346864	25.840324479459024	26.802973084542455	23.61492308465166
28	22.90476388091062	26.11395659191728	27.741202731952797	23.2400767952193
29	22.6802317835231	26.524186344167518	27.74426056207286	23.05132131023652
30	22.579410756135864	27.14470744210326	27.655889271603023	22.61999253015785
31	23.06647941097455	26.674631586074643	27.137150233377966	23.121738769572843
32	22.36938151017493	26.522963212119492	27.37282156906	23.734833708645578
33	22.47671134738916	26.268595429417633	27.742862696875115	23.51183052631809
34	23.15061342256373	26.08665453727386	27.441098547312276	23.32163349285014
35	23.08430219224578	26.184810884127895	27.76863583645851	22.962251087167814
36	22.497198809193584	26.423933199516863	27.565333816761715	23.51353417452784
37	23.13554268840056	25.507108362946962	27.685899690067078	23.671449258585405
38	22.51261900965619	25.519514416576932	28.429389242116805	23.53847733165007
39	22.57617819286608	25.52318381272101	28.290651121240778	23.60998687317213
40	22.09732199606414	25.4062436522723	28.950268761425914	23.54616559023766
41	21.427919299494803	25.620117027527023	29.30384128988011	23.648122383098062
42	22.10051087604649	25.351202710111153	29.029379194960697	23.51890721888166
43	21.40529135660633	25.12492328122645	29.09018633106253	24.379599031104686
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	529.0
1	498.5
2	468.0
3	1285.5
4	2103.0
5	2103.0
6	2848.0
7	3593.0
8	3595.0
9	3597.0
10	5393.0
11	7189.0
12	7189.0
13	11945.0
14	16701.0
15	22557.5
16	28414.0
17	27362.0
18	26310.0
19	26310.0
20	29982.0
21	33654.0
22	31539.0
23	29424.0
24	32215.0
25	35006.0
26	35006.0
27	39383.0
28	43760.0
29	52035.5
30	60311.0
31	66670.0
32	73029.0
33	73029.0
34	79749.0
35	86469.0
36	93722.5
37	100976.0
38	111726.5
39	122477.0
40	122477.0
41	128958.5
42	135440.0
43	134503.0
44	133566.0
45	139546.5
46	145527.0
47	145527.0
48	149777.5
49	154028.0
50	158248.0
51	162468.0
52	165708.0
53	168948.0
54	168948.0
55	157289.5
56	145631.0
57	138045.0
58	130459.0
59	119435.0
60	108411.0
61	108411.0
62	100892.5
63	93374.0
64	82883.0
65	72392.0
66	63293.5
67	54195.0
68	54195.0
69	46612.5
70	39030.0
71	33758.5
72	28487.0
73	22954.0
74	17421.0
75	17421.0
76	13850.0
77	10279.0
78	8439.5
79	6600.0
80	5208.5
81	3817.0
82	3817.0
83	2956.5
84	2096.0
85	1713.5
86	1331.0
87	1212.0
88	1093.0
89	1093.0
90	807.0
91	521.0
92	288.5
93	56.0
94	38.5
95	21.0
96	21.0
97	12.5
98	4.0
99	2.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	2289205.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	56.041224893875054
#Duplication Level	Percentage of deduplicated	Percentage of total
1	84.71172023982805	47.47348565107231
2	9.400133079936362	10.535899439301362
3	2.514752392009168	4.22789413058988
4	0.9901752916264939	2.2196254480959468
5	0.5419259653502017	1.5185097450010496
6	0.34592551377235636	1.1631653708307537
7	0.2295337647045077	0.9004347329980181
8	0.15616719020782338	0.7001440501984953
9	0.1240704554202034	0.6257754265400207
>10	0.7597331299789295	8.228773235956218
>50	0.10492388252983306	4.131334435014176
>100	0.1104800469358856	12.447523396002133
>500	0.008741890615075671	3.318283806686108
>1k	0.0014049467059942984	0.9781503339675787
>5k	1.5610518955492205E-4	0.6096708413755527
>10k+	1.5610518955492205E-4	0.9213299563705889
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	10842	0.473614202310409	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	10221	0.44648688081670274	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	8139	0.3555382763885279	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	5799	0.2533193838035475	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	4.3683287429478794E-4	0.0
3	0.0	0.0	0.0	8.29982461160097E-4	0.0
4	0.0	0.0	0.0	0.0015725983474612366	0.0
5	0.0	0.0	0.0	0.0017473314971791518	0.0
6	0.0	0.0	0.0	0.0017910147846086305	0.0
7	0.0	0.0	0.0	0.0021404810840444607	0.0
8	0.0	0.0	0.0	0.0024462640960508123	0.0
9	0.0	0.0	0.0	0.004062545730941528	0.0
10	0.0	0.0	0.0	0.0060719769526975526	0.0
11	0.0	0.0	0.0	0.007033009276146086	0.0
12	0.0	0.0	0.0	0.007906675024735662	0.0
13	0.0	4.368328742947879E-5	0.0	0.008605607623607322	0.0
14	0.0	8.736657485895759E-5	0.0	0.00939190679733794	0.0
15	0.0	8.736657485895759E-5	0.0	0.011008188432228656	0.0
16	0.0	8.736657485895759E-5	0.0	0.013978651977433214	0.0
17	0.0	8.736657485895759E-5	0.0	0.017866464558656828	0.0
18	0.0	8.736657485895759E-5	0.0	0.01983221249298337	0.0
19	0.0	1.3104986228843637E-4	0.0	0.02315214233762376	0.0
20	0.0	1.3104986228843637E-4	0.0	0.025467356571386136	0.0
21	0.0	1.3104986228843637E-4	0.0	0.031320917086936295	0.0
22	0.0	1.3104986228843637E-4	0.0	0.04163017292029329	0.0
23	0.0	1.7473314971791517E-4	0.0	0.05656985722117504	0.0
24	0.0	2.1841643714739397E-4	0.0	0.08090144831939472	0.0
25	0.0	2.1841643714739397E-4	0.0	0.08736657485895759	0.0
26	0.0	2.1841643714739397E-4	0.0	0.09916106246491686	0.0
27	0.0	2.1841643714739397E-4	0.0	0.11820697578416961	0.0
28	0.0	2.6209972457687274E-4	0.0	0.15918189939302071	0.0
29	0.0	3.4946629943583034E-4	0.0	0.2185474870096824	0.0
30	0.0	3.4946629943583034E-4	0.0	0.30984555773729305	0.0
31	0.0	3.4946629943583034E-4	0.0	0.6100807922401008	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
AAGACGG	415	0.0	18.27711	5
ACGGACC	395	0.0	17.797468	8
CGCAAGA	425	0.0	16.97647	2
CAAGACG	470	0.0	16.531916	4
CGGACCA	430	0.0	16.348837	9
GACGGAC	415	0.0	16.048193	7
TCTAGCG	225	0.0	15.622222	28
CTACGGT	85	5.367038E-4	15.235294	4
GCGCAAG	470	0.0	14.957447	1
GGTATCA	5865	0.0	14.509804	1
AGACGGA	490	0.0	14.346938	6
CTTATAC	1850	0.0	14.3	37
TATACTG	220	1.8189894E-11	14.295454	5
AACCGCG	195	6.730261E-10	14.23077	7
CTATACA	195	6.730261E-10	14.23077	4
GCGAAAG	470	0.0	14.170214	18
TAATACT	345	0.0	13.942028	4
CGCAATA	240	5.456968E-12	13.874999	36
GAGCGAA	495	0.0	13.828283	16
TAGGACC	690	0.0	13.673912	4
>>END_MODULE