FastQCFastQC Report
Fri 10 Feb 2017
ERR1630570.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1630570.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2142358
Sequences flagged as poor quality0
Sequence length43
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTGTAGCGCGCGTGCAGCCCCGGACATCTAAGGGCATCACAGA27090.12644945429288662No Hit
GGGTAGGCACACGCTGAGCCAGTCAGTGTAGCGCGCGTGCAGC26320.12285528375742989No Hit
GCTTAGGACACAGAGCACATCAAAAGTTCCCAAAGAGGGCTTG24050.11225948230874577No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA13750.021.2581831
CCGCTTA851.2463352E-619.58823625
GCGTTAT3900.018.974361
CGTTATT4050.016.9012342
AGTACCG904.4488414E-516.4444455
AAGACGG4600.016.0869565
CTAGACT1504.6766218E-916.0333334
GTTCAAA6200.015.8145151
TAGGACA8450.015.7633124
GACGGAC4750.015.5789477
CAAGACG5650.015.38937954
GCGCAAG5050.015.3861391
ACGGACC4750.015.1894738
CGCAAGA5000.014.7999992
TTAGGAC9450.014.682543
CCTATAC2650.014.6603773
TGGATAC6200.014.62096724
TACCGCA5850.014.54700828
GTATATA2301.8189894E-1214.4782611
CGCTTAT1152.2114966E-514.47826126
Produced by FastQC (version 0.11.5)