##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1630570.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	2142358
Sequences flagged as poor quality	0
Sequence length	43
%GC	51
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.0150002940685	33.0	31.0	34.0	30.0	34.0
2	32.17965624792868	33.0	31.0	34.0	30.0	34.0
3	32.29048272977719	34.0	31.0	34.0	30.0	34.0
4	35.878385405240394	37.0	35.0	37.0	35.0	37.0
5	35.85154115231908	37.0	35.0	37.0	35.0	37.0
6	35.883343026702356	37.0	35.0	37.0	35.0	37.0
7	35.851226545703376	37.0	35.0	37.0	35.0	37.0
8	35.83604140857877	37.0	35.0	37.0	35.0	37.0
9	37.595778109914406	39.0	37.0	39.0	35.0	39.0
10	37.48452826278334	39.0	37.0	39.0	35.0	39.0
11	37.598503144665834	39.0	37.0	39.0	35.0	39.0
12	37.50519194270986	39.0	37.0	39.0	35.0	39.0
13	37.55909843266158	39.0	37.0	39.0	35.0	39.0
14	38.84822097894003	40.0	38.0	41.0	35.0	41.0
15	38.829795487028775	40.0	38.0	41.0	35.0	41.0
16	38.82105371744592	40.0	38.0	41.0	35.0	41.0
17	38.78195941107882	40.0	38.0	41.0	35.0	41.0
18	38.75738228624721	40.0	38.0	41.0	35.0	41.0
19	38.78127278447393	40.0	38.0	41.0	35.0	41.0
20	38.76155759214846	40.0	38.0	41.0	35.0	41.0
21	38.68573973164149	40.0	38.0	41.0	34.0	41.0
22	38.63480940160328	40.0	38.0	41.0	34.0	41.0
23	38.61694870791903	40.0	38.0	41.0	34.0	41.0
24	38.573035412382055	40.0	38.0	41.0	34.0	41.0
25	38.53204926534221	40.0	38.0	41.0	34.0	41.0
26	38.35778380644131	40.0	38.0	41.0	34.0	41.0
27	38.25901973433012	40.0	38.0	41.0	34.0	41.0
28	38.160408297772825	40.0	38.0	41.0	33.0	41.0
29	38.073527860422956	40.0	38.0	41.0	33.0	41.0
30	38.012537120313226	40.0	37.0	41.0	33.0	41.0
31	37.978978303346125	40.0	37.0	41.0	33.0	41.0
32	37.89711990246261	40.0	37.0	41.0	33.0	41.0
33	37.88194223374431	40.0	37.0	41.0	33.0	41.0
34	37.81530024393682	40.0	37.0	41.0	33.0	41.0
35	37.77317049718114	40.0	37.0	41.0	33.0	41.0
36	37.74803557575345	40.0	37.0	41.0	33.0	41.0
37	37.58212119543045	40.0	37.0	41.0	32.0	41.0
38	37.48575914949789	40.0	37.0	41.0	32.0	41.0
39	37.44316169379721	40.0	36.0	41.0	32.0	41.0
40	37.36090653382861	40.0	36.0	41.0	32.0	41.0
41	37.26041632630961	39.0	36.0	41.0	31.0	41.0
42	37.1899668496115	39.0	36.0	41.0	31.0	41.0
43	36.30422599770907	38.0	35.0	40.0	30.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	2.0
9	0.0
10	3.0
11	3.0
12	2.0
13	1.0
14	2.0
15	2.0
16	10.0
17	35.0
18	58.0
19	126.0
20	253.0
21	468.0
22	946.0
23	1671.0
24	2853.0
25	4520.0
26	6878.0
27	10425.0
28	14923.0
29	21118.0
30	28727.0
31	38666.0
32	50744.0
33	66362.0
34	88877.0
35	120735.0
36	171395.0
37	260227.0
38	482555.0
39	769771.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	44.47347268757136	18.194344736033845	11.353237880877053	25.978944695517743
2	19.595324404231228	20.045202529175796	34.03497454673775	26.324498519855226
3	20.675722731681635	22.10372869520407	28.44645946195734	28.774089111156957
4	14.41780505405726	14.680599601000393	33.66038729288009	37.241208052062255
5	15.21879163053047	36.137190889664566	33.77129312654561	14.872724353259354
6	37.0712084534891	33.700343266624905	14.938026230910054	14.290422048975943
7	30.917568398932392	29.16057913756711	20.399905151239896	19.5219473122606
8	27.801609254849097	32.81701751061214	19.352694554318187	20.02867868022058
9	26.88010127158953	13.1387937963683	18.491820694767167	41.489284237275
10	17.08374604057772	25.846893936494276	32.490508122358634	24.578851900569372
11	37.72525413586338	20.585214982743313	20.523367243009805	21.1661636383835
12	22.694572989201617	24.442506807919127	27.477620453724356	25.385299749154903
13	30.719515599166897	18.766051238868574	24.626649700937005	25.88778346102752
14	23.660938087845263	20.007813820099162	23.439453163290167	32.89179492876541
15	26.535761063277008	27.043099239249464	21.41056723479456	25.010572462678976
16	27.466464521802614	25.187246949389415	22.243107827916717	25.103180700891258
17	25.375917563731175	25.279575122365173	23.54685818149908	25.797649132404576
18	24.84785456025557	23.779919135830706	25.572289972077495	25.799936331836232
19	26.08891697839483	24.441199836815322	24.410252628178856	25.059630556610983
20	26.240525626435918	23.704161489349584	24.69951333997399	25.355799544240504
21	26.31114874358067	24.526666411496116	23.82935065007809	25.332834194845123
22	26.54887745185445	23.673074248094856	24.029083841262757	25.74896445878793
23	24.954045962439515	24.167482745647554	24.738162342615006	26.140308949297925
24	25.774076974996706	24.8025773470167	23.99552269041869	25.427822987567904
25	26.014326270399252	24.237499054779825	24.18708731220459	25.56108736261633
26	26.152305076929256	24.858123618928303	23.78412011437864	25.2054511897638
27	26.680741500720234	23.694545916228755	23.570803759222315	26.0539088238287
28	25.172403491853366	24.17751841662318	24.92174510515983	25.728332986363622
29	25.07270026764901	24.758093651947995	24.8038843181205	25.365321762282495
30	24.880108739995837	25.140989507822688	24.593928745802522	25.384973006378953
31	25.57415707365436	24.680842324205386	23.969756688658013	25.77524391348225
32	24.82941693218407	24.43153758615507	24.14699130584151	26.592054175819353
33	24.870166424099054	23.914303771825253	24.767149094595766	26.44838070947993
34	25.957846447699218	23.97652493187413	24.723318885078964	25.342309735347683
35	26.099559457382938	23.900627252774747	24.746517622171456	25.253295667670855
36	24.72191855889632	24.882722682203443	24.265645611051	26.129713147849237
37	26.00933177368115	23.586767477704473	24.914883506864864	25.48901724174951
38	24.921325007305033	23.529914234689066	25.437345205609894	26.111415552396007
39	25.150231660628148	23.756160268265155	25.40705148252533	25.686556588581368
40	25.128293217100033	23.399310479387665	25.78705333095589	25.68534297255641
41	23.95337287232106	23.907955626463924	26.545329958858417	25.5933415423566
42	25.00333744406864	23.752239354953748	26.19935603666614	25.045067164311476
43	24.32445931072211	22.778312494923817	26.16019358109149	26.737034613262583
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	22.0
1	41.0
2	60.0
3	163.5
4	267.0
5	267.0
6	413.0
7	559.0
8	533.5
9	508.0
10	730.0
11	952.0
12	952.0
13	1596.5
14	2241.0
15	3407.0
16	4573.0
17	4856.0
18	5139.0
19	5139.0
20	6812.0
21	8485.0
22	9817.5
23	11150.0
24	13514.0
25	15878.0
26	15878.0
27	18789.0
28	21700.0
29	28354.5
30	35009.0
31	40286.0
32	45563.0
33	45563.0
34	55237.0
35	64911.0
36	73053.5
37	81196.0
38	94070.0
39	106944.0
40	106944.0
41	112884.5
42	118825.0
43	120818.0
44	122811.0
45	128246.0
46	133681.0
47	133681.0
48	141328.0
49	148975.0
50	154737.0
51	160499.0
52	172002.0
53	183505.0
54	183505.0
55	172842.0
56	162179.0
57	152810.5
58	143442.0
59	138404.0
60	133366.0
61	133366.0
62	125652.0
63	117938.0
64	104016.5
65	90095.0
66	80708.5
67	71322.0
68	71322.0
69	61860.5
70	52399.0
71	47765.5
72	43132.0
73	34809.5
74	26487.0
75	26487.0
76	18835.0
77	11183.0
78	9091.0
79	6999.0
80	5629.0
81	4259.0
82	4259.0
83	3318.5
84	2378.0
85	2030.5
86	1683.0
87	1436.0
88	1189.0
89	1189.0
90	970.5
91	752.0
92	407.5
93	63.0
94	44.0
95	25.0
96	25.0
97	18.5
98	12.0
99	7.0
100	2.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	2142358.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	50.383325232836384
#Duplication Level	Percentage of deduplicated	Percentage of total
1	86.68020285319214	43.672368516006124
2	7.874773036571712	7.935145020727262
3	2.0338687430315794	3.074192110831806
4	0.8951645340488494	1.804054634235345
5	0.5173128040335787	1.3031969626367177
6	0.32012483450528784	0.9677372191192706
7	0.22693993663082165	0.8003792044912977
8	0.16717698304996476	0.6738345846760599
9	0.1263921795343059	0.5731252459527583
>10	0.8530361997010393	8.632254044820053
>50	0.1274466347986041	4.60561555928484
>100	0.15455594033276693	16.366391836053033
>500	0.018181624321020603	6.168386237477841
>1k	0.004823696248434037	3.4233188236876946
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTGTAGCGCGCGTGCAGCCCCGGACATCTAAGGGCATCACAGA	2709	0.12644945429288662	No Hit
GGGTAGGCACACGCTGAGCCAGTCAGTGTAGCGCGCGTGCAGC	2632	0.12285528375742989	No Hit
GCTTAGGACACAGAGCACATCAAAAGTTCCCAAAGAGGGCTTG	2405	0.11225948230874577	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	4.667753942151592E-5	0.0
2	0.0	0.0	0.0	2.3338769710757958E-4	0.0
3	0.0	0.0	0.0	6.06808012479707E-4	0.0
4	0.0	0.0	0.0	0.001633713879753057	0.0
5	0.0	0.0	0.0	0.0020071341951251845	0.0
6	0.0	4.667753942151592E-5	0.0	0.0020538117345467004	0.0
7	0.0	4.667753942151592E-5	0.0	0.0025205871287618596	4.667753942151592E-5
8	0.0	9.335507884303184E-5	0.0	0.0028006523652909553	4.667753942151592E-5
9	1.4003261826454774E-4	1.8671015768606368E-4	0.0	0.004247656087357949	4.667753942151592E-5
10	2.3338769710757958E-4	1.8671015768606368E-4	0.0	0.00686159829496284	9.335507884303184E-5
11	2.3338769710757958E-4	2.3338769710757958E-4	0.0	0.007701794004550126	9.335507884303184E-5
12	2.3338769710757958E-4	2.3338769710757958E-4	0.0	0.008355279556451349	9.335507884303184E-5
13	2.3338769710757958E-4	2.3338769710757958E-4	0.0	0.008728699871823477	9.335507884303184E-5
14	2.3338769710757958E-4	2.3338769710757958E-4	0.0	0.009288830344881668	9.335507884303184E-5
15	2.800652365290955E-4	2.3338769710757958E-4	0.0	0.009895638357361375	9.335507884303184E-5
16	2.800652365290955E-4	2.800652365290955E-4	0.0	0.011342642079428368	9.335507884303184E-5
17	3.267427759506114E-4	2.800652365290955E-4	0.0	0.012416225486123235	9.335507884303184E-5
18	3.267427759506114E-4	2.800652365290955E-4	0.0	0.013303098735132036	9.335507884303184E-5
19	3.267427759506114E-4	2.800652365290955E-4	0.0	0.014096616905297807	9.335507884303184E-5
20	3.267427759506114E-4	2.800652365290955E-4	0.0	0.015123522772571157	9.335507884303184E-5
21	3.267427759506114E-4	2.800652365290955E-4	0.0	0.016663881573481185	9.335507884303184E-5
22	3.267427759506114E-4	2.800652365290955E-4	0.0	0.01918446870224304	9.335507884303184E-5
23	3.7342031537212735E-4	2.800652365290955E-4	0.0	0.023012026934807348	9.335507884303184E-5
24	4.200978547936433E-4	3.267427759506114E-4	0.0	0.028566654125967744	9.335507884303184E-5
25	4.200978547936433E-4	3.267427759506114E-4	0.0	0.03076049847877899	9.335507884303184E-5
26	4.200978547936433E-4	3.267427759506114E-4	0.0	0.03374786100175601	9.335507884303184E-5
27	4.200978547936433E-4	3.267427759506114E-4	0.0	0.03948919835060247	9.335507884303184E-5
28	4.200978547936433E-4	4.200978547936433E-4	0.0	0.055546271911603946	9.335507884303184E-5
29	4.200978547936433E-4	5.60130473058191E-4	0.0	0.08453302389236533	9.335507884303184E-5
30	4.200978547936433E-4	5.60130473058191E-4	0.0	0.12476906287371205	9.335507884303184E-5
31	4.200978547936433E-4	5.60130473058191E-4	0.0	0.2797384937531449	9.335507884303184E-5
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	1375	0.0	21.258183	1
CCGCTTA	85	1.2463352E-6	19.588236	25
GCGTTAT	390	0.0	18.97436	1
CGTTATT	405	0.0	16.901234	2
AGTACCG	90	4.4488414E-5	16.444445	5
AAGACGG	460	0.0	16.086956	5
CTAGACT	150	4.6766218E-9	16.033333	4
GTTCAAA	620	0.0	15.814515	1
TAGGACA	845	0.0	15.763312	4
GACGGAC	475	0.0	15.578947	7
CAAGACG	565	0.0	15.3893795	4
GCGCAAG	505	0.0	15.386139	1
ACGGACC	475	0.0	15.189473	8
CGCAAGA	500	0.0	14.799999	2
TTAGGAC	945	0.0	14.68254	3
CCTATAC	265	0.0	14.660377	3
TGGATAC	620	0.0	14.620967	24
TACCGCA	585	0.0	14.547008	28
GTATATA	230	1.8189894E-12	14.478261	1
CGCTTAT	115	2.2114966E-5	14.478261	26
>>END_MODULE